FastQCFastQC Report
Sun 19 Mar 2023
SRR6026834_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026834_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188671843
Sequences flagged as poor quality0
Sequence length150-151
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2411720.12782617488927586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGGG10253000.0137.733962
ACATCGG10572150.0137.294111
ATGCCTA2689700.0135.834561
ATCGGGC5127800.0135.4583
CTGGCAT3831650.0135.395051
GACTAGT3454300.0135.369231
AGGCTAA3947250.0134.45651
GGCTAAC3677600.0134.37572
TGGCATA3582950.0132.694122
TGCCTAA2496900.0131.899542
ACTAGTA3259200.0131.366562
CGAACTT2053000.0131.350251
ATCATTC3369250.0130.06751
CATCGGT3042950.0128.76842
ATCGGTC1489700.0126.839283
AGTGGTC3802300.0126.738341
GCTAACT1393750.0126.550163
ATGAATC2917850.0126.430732
GTGGTCA3496600.0124.6306462
AGTTAGC1425800.0124.247782
Produced by FastQC (version 0.11.5)