FastQCFastQC Report
Sat 31 Dec 2022
SRR5849872.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849872.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150630
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGC38722.5705370776073826No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCCGTCT36572.427803226448914No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGC8950.5941711478457147No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCT4470.2967536347341167No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCTTATAC4300.2854677023169355No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTA3820.25360154019783576No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTATACAC3600.23899621589324838No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCA3100.2058022970191861No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGC3070.20381066188674235No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAT2890.19186085109207995No Hit
AGAACAGGGGGGATGGGCTGGTGAGAAAGTTCAAATTCCAGGAAGCCACTTT2660.1765916484100113No Hit
CCATATGGGGAAATCAGTGGGATTCCTTTGGATGATATTGAATTTGCTAAGG2550.1692889862577176No Hit
GGCTTAGGTATGAAAATTGAAATATAAATAAAAATTTAATTTTCAGTGACCT2420.1606585673504614No Hit
GACGACGGGGGAGGACACATTATGAAATTATTAAATATGTGTTCAAATTTCA2370.15733917546305518No Hit
TGATCCGGGGAACACTTGTTATGTCCCATATTTTTGGTAGTGGTCATCCTAA2330.15468366195313019No Hit
CGTAGGGGTTACATTACAACTGACGCCACAGAAATACAAAAGATCCTCAGAG2280.15136427006572395No Hit
GAACGTGGGGACTTTCACTGCTTCCTATAATACCTCTTAGCTGCTTTTCTGC2160.14339772953594904No Hit
AGTCTTGGGGAAGCCCACTATCAGGACCCTTCACTTCCTTACCCCAAAGAAG2150.14273385115846776No Hit
TAATGAGGGCGAGACGTTCTTCGAATCCTCATGGAAGGTACCCCCCGCAATG2120.14074221602602405No Hit
GACATGGGGAACACAGGATTAAATCACTGTGCAAAAACCAGGCTTTGAGGCA2050.13609506738365532No Hit
AGTCTAGGGGGGAGACAGGCCCAGGTATGAATGAGCCAGCCATGCTTCCTCC2010.13343955387373035No Hit
TGCATAGGGACAGTGAAAGAGTAAATTAGAAAGTGAATGGATGACTGTTCAT2000.13277567549624908No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGC1980.13144791874128658No Hit
GTTCATGGGCAGGCTTTCAGATGATTGGTTGAGGCTCACTCACATTAGAAGG1950.12945628360884284No Hit
TCGCCAGGGATATTATTGATTTTTAATTTTATATAAGCAATTTTTTATTTCC1950.12945628360884284No Hit
CATGTAGGGAACCTAGGAGCATTAAGACTTGTCACACAGTAAACCTGATACA1930.12812852685388038No Hit
GCTCATGGGGAAGCATGTGATCTCTGTGACCCACACCCTATTCATACACTCC1920.12746464847639913No Hit
ATGTGCGGGGTGGCCCCCCAGGACTGCGGCTGGCCGCCAGCTGGTGGGTGGA1910.12680077009891788No Hit
GCTGGGGCGGGCGAGGCCTATATTACGGATCATTTCTCTACTCAGAAACCTG1910.12680077009891788No Hit
AAGCTTGGGACCTATGAATACATCGACATCCCATTCCAGAACAAATACTCCC1910.12680077009891788No Hit
TGTTTGGGGCGATCTCGTAGCGGGGTGATTCTCACCTCCCTCCCCACCTCAG1900.12613689172143663No Hit
ATCGCTGGGGGAAGTGCTCAGGATTAGAAAGCATTTGAAATCTTGGGAATGG1890.1254730133439554No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGT1880.12480913496647414No Hit
GCCAGGGGGAATGCACTTTCCTAACAGTGAATTCACAACCCTGAACAGCAGC1870.12414525658899289No Hit
GTAACTGGGGGAATTTGTAAAAGAGAAAAGCATTGTCCTTATGCCTAAAGTA1860.12348137821151166No Hit
ATCGAGGGGAGCACAGGCGGGAATAGCACAGCTGCCCTCCCATGCTCCCTAC1860.12348137821151166No Hit
TCGCCTGGGCCTTCTCATATATTTAGCTCCCACATATTTGTGAGAACATGTG1840.12215362145654915No Hit
GCATGCGGGTGACTCAGCTCTTCTGTGTCTCTGTTTTTCTTGCACTTTGTCT1830.12148974307906792No Hit
CGCCGAGGGTGGAAGGATGTCTGTTTATCTCTCATCTCTGAATAGATGTATG1830.12148974307906792No Hit
AAGGAGGGGATTGTGAGCAGTTTTCTTTGGTAGGTGGTTAGTGATAGGAGCC1830.12148974307906792No Hit
GCGCACGGGGAGGCTTGCTGCGTGGAGATGGAAATCAATACGGGAACTGATA1820.12082586470158667No Hit
GTCTCAGGGACTTTTTCACTATTATAAATAATGCTGCTATGACCTTTCTCAT1810.12016198632410544No Hit
AATGTGGGGATATATAGGTAAATATATGAAGTAAATATATATGTACACACAC1810.12016198632410544No Hit
GTACAAGGGATACATATATGTTTGTATGTATACATACCCACATTAACATTTT1810.12016198632410544No Hit
TTGCGTGGGGTAATACAATGAAAGATCATGAAAGATCATCTGTCTGTCAGCA1800.11949810794662419No Hit
GAAACTGGGATGGTAAGTGTGTTTTTCTCCATTTCTATTTAGGCCAACACAC1790.11883422956914293No Hit
ACTAGGGGTATTTTAAATTTGAACCACTAGAGTTTTTTATGATGCAAATGAT1790.11883422956914293No Hit
TTGCGTGGGAAGGCTACTGCAGGTCACTGAGTTGCCTGCCATCCAAAGGGGA1780.11817035119166168No Hit
GAGTGTGGGCTGCAAAGTACAGGTACTTTGTCTAAGAAACATTGGAAGCAGG1780.11817035119166168No Hit
CGTTCAGGGAATATTGTACTGAAAGTCTGGCTGTATCATAAATATAACATTG1770.11750647281418045No Hit
GGTAACGGGGGGGCTATGTATTTAGCAAGAAACTCTTTAAAGTTGAAATTTA1770.11750647281418045No Hit
CGTTCTGGGGGAGAGCGCGCGGGGAGGGTGTGTCACGCCTCCCGAGACTGGG1760.1168425944366992No Hit
ACCGCAGGGATCACGAGAATAGCATGGGAAAGACCAGCCCCCATGATTCAAT1740.11551483768173669No Hit
ACTCTTGGGACCTGTACAATGCAGGAAGTCTAATAGCAGATGCCCATGTAGA1740.11551483768173669No Hit
TGGCGCGGGAGAGCCTCTCTGGGATGAAGTTCAAGCCAGAAAACCCAGTCGA1730.11485095930425546No Hit
AAGCCAGGGAAACCTTTCAGAAATAGATACTATTTCCTTTGCAAGGCTATAC1730.11485095930425546No Hit
GCATAGGGGCTTTCCTGACAAGTTTGTTTTAAATCAAGCTGCTAATTAGTCC1720.11418708092677421No Hit
TAATAGAGGGTGGAATTAAAAGAGAAGGAAAAACACAAGGATGAAGTCTTTC1710.11352320254929298No Hit
CAAGCTGGGTAGATAAGAAGGTTGAGAAGGTGACAATCTCCAATAGACTTGT1710.11352320254929298No Hit
CATCAAGGGGATATTTTAACATCTACTTTCATGACAGCCATGGATACTGCAA1710.11352320254929298No Hit
CAACACGGGGTACTGATCATGGAGTTAATCAACAATGTCGCCAAAGCCCATG1710.11352320254929298No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCC1700.11285932417181173No Hit
TCGTATGGGATTGCGTGTTAGAAGATTATTTAAATAATTGTTCTTTTGTTAT1700.11285932417181173No Hit
GTGCACGGGATTTGATATTTGTTTAGTGTGTTTCATGCAAGCAATAGAATAG1700.11285932417181173No Hit
TGTTATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1690.11219544579433047No Hit
TATCCAGGGGAAGTTGTGGCAGGGATAAAAGAATACTTCAACGTAATGTTGG1670.11086768903936799No Hit
ATTTACGGGCCAGAAGAATTGACCTTGACCATTAATAAAGACACTGGCAGAG1670.11086768903936799No Hit
TCATGCGGGATATTAGCACTGTTTGTAAACACCTGGTATGATAGCAATTTTT1670.11086768903936799No Hit
AATGTGGGGCAAATAGAAAGGCAGAGAAGGAACTAAGTATAGAAATCATGTC1660.11020381066188674No Hit
GCATATGGGAAAAGAAAACTAACTGGAAAACAGGTTAGATTAATTCAGTACT1650.1095399322844055No Hit
CCCGGATTCCCTCCACAACATGAAATCTAACCCCATGAAACCCATGATAGTC1650.1095399322844055No Hit
TGGTGCGGGCTCAAGACTCTGGCTGGTGATGTTCATATTGTGAGAGGAGACT1640.10887605390692426No Hit
TCTAAGGGGATTCACAAAACGCCGCATGTTTAAAAAGTCATAGATGTCATCT1630.108212175529443No Hit
AACTGTGGGATAGGTCCTTACAAAACCATTTAAAAATGTGAAGACTATTCTT1630.108212175529443No Hit
GTAACAGGGAATGCAATGCTGTAGTCTTGATATACACACAGTGTGAACTTTA1620.10754829715196176No Hit
ATCGGGCGGGGTCGACTGACGGTAACGGGGCAGAGAGGCTGTTCGCAGAGCT1620.10754829715196176No Hit
AGACGAGGGAGAATATAAATATTTCCCCTCACCTCCTGCAAAGGTTTGAATT1610.10688441877448052No Hit
TCTATGGGGAAGAGCAATGAGAAAATGATGGAATGTTGACTGTGTTTGGCAC1600.10622054039699927No Hit
GAACACGGGATTGATCAGCAGCAGACTAATAAGATGGACTTACTGGAATCAG1600.10622054039699927No Hit
CTTCAAGGGATCTACTGGTGCTACCAAGGCTGTAGGCAAAGTCATCCTGGAG1590.10555666201951804No Hit
GCACCTGGTGCTCCGTCTTAGAGGTGGGATGCAGATCTTCGTGAAGACCCTG1590.10555666201951804No Hit
TTTTAAGGGGGAAGCAGATATTGATGGTGATGGTCAAGTAAACTATGAAGAG1590.10555666201951804No Hit
AGCCATGGGGTTCAGCATTATTATGCAAAAATTCACTAGTTGAGATGGTTTG1580.10489278364203677No Hit
CATGCTGGGGTTATGACAAATGTGCTCTCTTTCTTGTAAATAGACATGAGTG1580.10489278364203677No Hit
CATCAAGGGGTACATTTGGACGCTGTCCTGCGCTCCAGCTGCAAGCTGGGCT1570.10422890526455553No Hit
GAGTACGGGACGAAAGACCCAGACTAGATGTCTGTTTCTCTGGAAGCTTTAT1560.1035650268870743No Hit
TCGCAGGGTGGGCCATGAGAACTAGGGGAAAGGAGTTTGAATGTGTCTATTT1550.10290114850959305No Hit
CAACGAGGGGAGATTGGGAGGAATAGAGGAGGCTGGTACAAATAGATGGAGA1550.10290114850959305No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCC1530.10157339175463054No Hit
AGAACGGGGTATTTCTTATATGTTAAAAATATTAAAAGCATTTTTATACATT1530.10157339175463054No Hit
AGGCCCAAACAAACTAAAGCAAGTGACATGGACTGTTTATTGATGCTTTTTT1520.1009095133771493No Hit
GGTCATGGGCCATCTCTAAGGATGTGACAGAGAAGGATATAGTGGACTTACT1520.1009095133771493No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTCTTATA1510.10024563499966806No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)