FastQCFastQC Report
Sat 31 Dec 2022
SRR5849871.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849871.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147831
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGC37522.5380332947757913No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCCGTCT35172.3790679897991622No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGC8550.5783631308724151No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCTTATAC4370.29560782244590106No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCT4120.27869661978881294No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTATACAC3610.24419776636835305No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTA3390.22931590803011545No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAT3180.2151104977981614No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGC2780.18805257354682037No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCA2760.1866996773342533No Hit
TAATGAGGGCGAGACGTTCTTCGAATCCTCATGGAAGGTACCCCCCGCAATG2600.17587650763371687No Hit
CCATATGGGGAAATCAGTGGGATTCCTTTGGATGATATTGAATTTGCTAAGG2520.17046492278344866No Hit
AGAACAGGGGGGATGGGCTGGTGAGAAAGTTCAAATTCCAGGAAGCCACTTT2490.16843557846459808No Hit
GACATGGGGAACACAGGATTAAATCACTGTGCAAAAACCAGGCTTTGAGGCA2440.16505333793318044No Hit
GACGACGGGGGAGGACACATTATGAAATTATTAAATATGTGTTCAAATTTCA2220.15017147959494287No Hit
TGCATAGGGACAGTGAAAGAGTAAATTAGAAAGTGAATGGATGACTGTTCAT2190.1481421352760923No Hit
GCTCATGGGGAAGCATGTGATCTCTGTGACCCACACCCTATTCATACACTCC2130.14408344663839112No Hit
TGATCCGGGGAACACTTGTTATGTCCCATATTTTTGGTAGTGGTCATCCTAA2110.14273055042582408No Hit
CGTAGGGGTTACATTACAACTGACGCCACAGAAATACAAAAGATCCTCAGAG2080.1407012061069735No Hit
TTGCGTGGGAAGGCTACTGCAGGTCACTGAGTTGCCTGCCATCCAAAGGGGA2060.13934830989440647No Hit
TATCCAGGGGAAGTTGTGGCAGGGATAAAAGAATACTTCAACGTAATGTTGG2060.13934830989440647No Hit
GGCTTAGGTATGAAAATTGAAATATAAATAAAAATTTAATTTTCAGTGACCT2050.1386718617881229No Hit
GAACGTGGGGACTTTCACTGCTTCCTATAATACCTCTTAGCTGCTTTTCTGC2040.1379954136818394No Hit
ATGTGCGGGGTGGCCCCCCAGGACTGCGGCTGGCCGCCAGCTGGTGGGTGGA2010.13596606936298883No Hit
AAGGAGGGGATTGTGAGCAGTTTTCTTTGGTAGGTGGTTAGTGATAGGAGCC2010.13596606936298883No Hit
AGTCTTGGGGAAGCCCACTATCAGGACCCTTCACTTCCTTACCCCAAAGAAG2000.1352896212567053No Hit
TGTTATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1990.13461317315042176No Hit
GTTCATGGGCAGGCTTTCAGATGATTGGTTGAGGCTCACTCACATTAGAAGG1970.13326027693785472No Hit
TGTTTGGGGCGATCTCGTAGCGGGGTGATTCTCACCTCCCTCCCCACCTCAG1960.1325838288315712No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGT1930.13055448451272061No Hit
GCATGCGGGTGACTCAGCTCTTCTGTGTCTCTGTTTTTCTTGCACTTTGTCT1920.12987803640643708No Hit
AGTCTAGGGGGGAGACAGGCCCAGGTATGAATGAGCCAGCCATGCTTCCTCC1920.12987803640643708No Hit
CATGTAGGGAACCTAGGAGCATTAAGACTTGTCACACAGTAAACCTGATACA1910.12920158830015355No Hit
CGCCGAGGGTGGAAGGATGTCTGTTTATCTCTCATCTCTGAATAGATGTATG1900.12852514019387No Hit
GCCAGGGGGAATGCACTTTCCTAACAGTGAATTCACAACCCTGAACAGCAGC1880.12717224398130297No Hit
GCTGGGGCGGGCGAGGCCTATATTACGGATCATTTCTCTACTCAGAAACCTG1880.12717224398130297No Hit
TCGCCTGGGCCTTCTCATATATTTAGCTCCCACATATTTGTGAGAACATGTG1870.12649579587501944No Hit
CAACACGGGGTACTGATCATGGAGTTAATCAACAATGTCGCCAAAGCCCATG1850.1251428996624524No Hit
TCGCAGGGTGGGCCATGAGAACTAGGGGAAAGGAGTTTGAATGTGTCTATTT1840.12446645155616888No Hit
AACTGTGGGATAGGTCCTTACAAAACCATTTAAAAATGTGAAGACTATTCTT1830.12379000344988533No Hit
GAAACTGGGATGGTAAGTGTGTTTTTCTCCATTTCTATTTAGGCCAACACAC1820.12311355534360181No Hit
AAGCTTGGGACCTATGAATACATCGACATCCCATTCCAGAACAAATACTCCC1810.1224371072373183No Hit
AATGTGGGGCAAATAGAAAGGCAGAGAAGGAACTAAGTATAGAAATCATGTC1800.12176065913103477No Hit
TTGCGTGGGGTAATACAATGAAAGATCATGAAAGATCATCTGTCTGTCAGCA1770.11973131481218419No Hit
TTCCATGGGAGGGGTTCTGTATGGCCCATAATATAAAAATATAGGCCATCTG1770.11973131481218419No Hit
GTGCACGGGATTTGATATTTGTTTAGTGTGTTTCATGCAAGCAATAGAATAG1760.11905486670590065No Hit
ACTCTTGGGACCTGTACAATGCAGGAAGTCTAATAGCAGATGCCCATGTAGA1730.11702552238705008No Hit
TCAACGGGGAATGCAGGAAGGCAACAGATGTGTTTTCTTATGGCTCTGGTAA1730.11702552238705008No Hit
TGGTTTGGGAAAGTGAAATTTAAATAGATAATTATAGGTTGATTACAAAATC1730.11702552238705008No Hit
ATCGAGGGGAGCACAGGCGGGAATAGCACAGCTGCCCTCCCATGCTCCCTAC1720.11634907428076655No Hit
GAGTGTGGGCTGCAAAGTACAGGTACTTTGTCTAAGAAACATTGGAAGCAGG1720.11634907428076655No Hit
AGACGAGGGAGAATATAAATATTTCCCCTCACCTCCTGCAAAGGTTTGAATT1720.11634907428076655No Hit
TGACAAGGGGAAGGAGCGTAAGCTTTCTGGGAGGGTTTTAAGCTTCTCAAGA1710.11567262617448303No Hit
GTAACTGGGGGAATTTGTAAAAGAGAAAAGCATTGTCCTTATGCCTAAAGTA1710.11567262617448303No Hit
CATCAAGGGGATATTTTAACATCTACTTTCATGACAGCCATGGATACTGCAA1690.11431972996191597No Hit
CGTTCTGGGGGAGAGCGCGCGGGGAGGGTGTGTCACGCCTCCCGAGACTGGG1680.11364328185563244No Hit
ACGCTTGGGGCCTCTGCGCGGTGCCAGAGAGCTAAAGTCATGGTCTAACAGG1680.11364328185563244No Hit
TGGCGCGGGAGAGCCTCTCTGGGATGAAGTTCAAGCCAGAAAACCCAGTCGA1670.11296683374934892No Hit
TCGCCAGGGATATTATTGATTTTTAATTTTATATAAGCAATTTTTTATTTCC1660.1122903856430654No Hit
TACAACGGGAACTGTAAGTTTATGTACCCCAGAGGACCTAGTCCACAGCCCT1640.11093748943049833No Hit
CATGCTGGGGTTATGACAAATGTGCTCTCTTTCTTGTAAATAGACATGAGTG1630.11026104132421481No Hit
GTACAAGGGATACATATATGTTTGTATGTATACATACCCACATTAACATTTT1630.11026104132421481No Hit
CCCGGATTCCCTCCACAACATGAAATCTAACCCCATGAAACCCATGATAGTC1620.10958459321793129No Hit
ATTTACGGGCCAGAAGAATTGACCTTGACCATTAATAAAGACACTGGCAGAG1620.10958459321793129No Hit
GCATAGGGGCTTTCCTGACAAGTTTGTTTTAAATCAAGCTGCTAATTAGTCC1610.10890814511164774No Hit
TCATGCGGGATATTAGCACTGTTTGTAAACACCTGGTATGATAGCAATTTTT1610.10890814511164774No Hit
GAGTACGGGACGAAAGACCCAGACTAGATGTCTGTTTCTCTGGAAGCTTTAT1610.10890814511164774No Hit
CGTTCAGGGAATATTGTACTGAAAGTCTGGCTGTATCATAAATATAACATTG1600.10823169700536422No Hit
AATGTGGGGATATATAGGTAAATATATGAAGTAAATATATATGTACACACAC1600.10823169700536422No Hit
AGCCATGGGGTTCAGCATTATTATGCAAAAATTCACTAGTTGAGATGGTTTG1590.1075552488990807No Hit
ATCGCTGGGGGAAGTGCTCAGGATTAGAAAGCATTTGAAATCTTGGGAATGG1580.10687880079279719No Hit
TAATAGAGGGTGGAATTAAAAGAGAAGGAAAAACACAAGGATGAAGTCTTTC1580.10687880079279719No Hit
CAAGCTGGGTAGATAAGAAGGTTGAGAAGGTGACAATCTCCAATAGACTTGT1580.10687880079279719No Hit
GGTCATGGGCCATCTCTAAGGATGTGACAGAGAAGGATATAGTGGACTTACT1580.10687880079279719No Hit
TTTTAAGGGGGAAGCAGATATTGATGGTGATGGTCAAGTAAACTATGAAGAG1580.10687880079279719No Hit
TCTATGGGGAAGAGCAATGAGAAAATGATGGAATGTTGACTGTGTTTGGCAC1570.10620235268651367No Hit
AACTAGGGGGCTGTGACTGGACCACTTCCACCTCTTGGTAGCCATTGCTTTG1560.10552590458023012No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCC1560.10552590458023012No Hit
GTTTATGGGATTTTCAAACAAGTATACAGGCATTCCTTGGAGAAAATGTGCG1560.10552590458023012No Hit
GGTAACGGGGGGGCTATGTATTTAGCAAGAAACTCTTTAAAGTTGAAATTTA1560.10552590458023012No Hit
ACTAGGGGTATTTTAAATTTGAACCACTAGAGTTTTTTATGATGCAAATGAT1560.10552590458023012No Hit
TCTAAGGGGATTCACAAAACGCCGCATGTTTAAAAAGTCATAGATGTCATCT1550.1048494564739466No Hit
CTTTGAGGGATATGCTCAATCAATTATGAGAGATACTATGAACAACTTTTTA1540.10417300836766308No Hit
TTTATTGGGAATTGGTGGAGCTAAAATCCTTCAGTTCTCACAACTGGGACTC1530.10349656026137956No Hit
AGTTAAGGGGGGCTACAACCCTGGGCAAGTTACTTCACCTCTGAGCCTCCGA1530.10349656026137956No Hit
AAGCCAGGGAAACCTTTCAGAAATAGATACTATTTCCTTTGCAAGGCTATAC1520.10282011215509601No Hit
TTCTGTGGGCAAGTAAACTATGAAGAGTTTGTACAAATGATGACAGCAAAGT1510.10214366404881249No Hit
GCGCACGGGGAGGCTTGCTGCGTGGAGATGGAAATCAATACGGGAACTGATA1510.10214366404881249No Hit
GTAAAAGGGATAATACACTTGCCCTGCTCCCTCTACCCCTTACCCCACAACG1500.10146721594252897No Hit
AGAACGGGGTATTTCTTATATGTTAAAAATATTAAAAGCATTTTTATACATT1500.10146721594252897No Hit
ACTGCAGGGGAATGTGGGTAAGGAAACAGCTGCTCCAATTTCCTATGAATTA1490.10079076783624545No Hit
GATAGCGGGAAGGTTTTAATGTGAGATATTCCTGCAAACCATCCCATTTCTA1480.10011431972996193No Hit
AGCTACGGGAATGCCCTGTCTTCCAATATAATGACCTAATTTCTATTGATAT1480.10011431972996193No Hit
GAACACGGGATTGATCAGCAGCAGACTAATAAGATGGACTTACTGGAATCAG1480.10011431972996193No Hit
GAGTGAGGGCAGATAACTAGGATAACTTGTTGCTTTGTTACCCAGCCTAATT1480.10011431972996193No Hit
TAGAAGGGGATAGAATGAATGTAGTAACAGAAATTAACTCTTTACTGCATCA1480.10011431972996193No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)