FastQCFastQC Report
Sat 31 Dec 2022
SRR5849870.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849870.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147229
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGC36642.4886401456234846No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCCGTCT35662.422077172296219No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGC8780.5963499038912171No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGC3930.2669311073226063No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCTTATAC3900.2648934652819757No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCT3660.24859232895693106No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTATACAC3570.2424794028350393No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTA3250.2207445544016464No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCA2680.18202935562966535No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAT2670.1813501416161218No Hit
AGAACAGGGGGGATGGGCTGGTGAGAAAGTTCAAATTCCAGGAAGCCACTTT2600.17659564352131712No Hit
CCATATGGGGAAATCAGTGGGATTCCTTTGGATGATATTGAATTTGCTAAGG2300.15621922311501132No Hit
GAACGTGGGGACTTTCACTGCTTCCTATAATACCTCTTAGCTGCTTTTCTGC2170.14738944093894546No Hit
TAATGAGGGCGAGACGTTCTTCGAATCCTCATGGAAGGTACCCCCCGCAATG2160.14671022692540192No Hit
CGTAGGGGTTACATTACAACTGACGCCACAGAAATACAAAAGATCCTCAGAG2140.14535179889831487No Hit
GCATGCGGGTGACTCAGCTCTTCTGTGTCTCTGTTTTTCTTGCACTTTGTCT2130.14467258488477136No Hit
TGATCCGGGGAACACTTGTTATGTCCCATATTTTTGGTAGTGGTCATCCTAA2130.14467258488477136No Hit
AGTCTTGGGGAAGCCCACTATCAGGACCCTTCACTTCCTTACCCCAAAGAAG2120.14399337087122782No Hit
TCAACGGGGAATGCAGGAAGGCAACAGATGTGTTTTCTTATGGCTCTGGTAA2100.14263494284414074No Hit
AAGGAGGGGATTGTGAGCAGTTTTCTTTGGTAGGTGGTTAGTGATAGGAGCC1980.13448437468161842No Hit
AATGTGGGGATATATAGGTAAATATATGAAGTAAATATATATGTACACACAC1910.12972987658681376No Hit
TTGCGTGGGGTAATACAATGAAAGATCATGAAAGATCATCTGTCTGTCAGCA1880.12769223454618317No Hit
CATGTAGGGAACCTAGGAGCATTAAGACTTGTCACACAGTAAACCTGATACA1870.12701302053263963No Hit
GCTGGGGCGGGCGAGGCCTATATTACGGATCATTTCTCTACTCAGAAACCTG1860.1263338065190961No Hit
GGCTTAGGTATGAAAATTGAAATATAAATAAAAATTTAATTTTCAGTGACCT1860.1263338065190961No Hit
ACTCTTGGGACCTGTACAATGCAGGAAGTCTAATAGCAGATGCCCATGTAGA1850.12565459250555255No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGT1850.12565459250555255No Hit
GTTCATGGGCAGGCTTTCAGATGATTGGTTGAGGCTCACTCACATTAGAAGG1850.12565459250555255No Hit
AATGTGGGGCAAATAGAAAGGCAGAGAAGGAACTAAGTATAGAAATCATGTC1850.12565459250555255No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCC1840.12497537849200904No Hit
TCGCCTGGGCCTTCTCATATATTTAGCTCCCACATATTTGTGAGAACATGTG1840.12497537849200904No Hit
TATCCAGGGGAAGTTGTGGCAGGGATAAAAGAATACTTCAACGTAATGTTGG1830.12429616447846553No Hit
CCCGGATTCCCTCCACAACATGAAATCTAACCCCATGAAACCCATGATAGTC1830.12429616447846553No Hit
AAGCTTGGGACCTATGAATACATCGACATCCCATTCCAGAACAAATACTCCC1820.12361695046492198No Hit
AGAACGGGGTATTTCTTATATGTTAAAAATATTAAAAGCATTTTTATACATT1810.12293773645137847No Hit
GACATGGGGAACACAGGATTAAATCACTGTGCAAAAACCAGGCTTTGAGGCA1800.12225852243783494No Hit
TGGCGCGGGAGAGCCTCTCTGGGATGAAGTTCAAGCCAGAAAACCCAGTCGA1790.1215793084242914No Hit
TACAACGGGAACTGTAAGTTTATGTACCCCAGAGGACCTAGTCCACAGCCCT1790.1215793084242914No Hit
TGTTTGGGGCGATCTCGTAGCGGGGTGATTCTCACCTCCCTCCCCACCTCAG1790.1215793084242914No Hit
GTAACTGGGGGAATTTGTAAAAGAGAAAAGCATTGTCCTTATGCCTAAAGTA1780.12090009441074788No Hit
CGCCGAGGGTGGAAGGATGTCTGTTTATCTCTCATCTCTGAATAGATGTATG1780.12090009441074788No Hit
GACGACGGGGGAGGACACATTATGAAATTATTAAATATGTGTTCAAATTTCA1780.12090009441074788No Hit
CAGCGGGGGAGATTAAATTGGAGAAACGTGTTAATTGTGTGGAATGAAAAAA1740.11818323835657378No Hit
CGTTCTGGGGGAGAGCGCGCGGGGAGGGTGTGTCACGCCTCCCGAGACTGGG1740.11818323835657378No Hit
AAGCCAGGGAAACCTTTCAGAAATAGATACTATTTCCTTTGCAAGGCTATAC1730.11750402434303024No Hit
TGCATAGGGACAGTGAAAGAGTAAATTAGAAAGTGAATGGATGACTGTTCAT1730.11750402434303024No Hit
ATCGCTGGGGGAAGTGCTCAGGATTAGAAAGCATTTGAAATCTTGGGAATGG1720.11682481032948672No Hit
AGGCCCAAACAAACTAAAGCAAGTGACATGGACTGTTTATTGATGCTTTTTT1710.11614559631594318No Hit
AGTCTAGGGGGGAGACAGGCCCAGGTATGAATGAGCCAGCCATGCTTCCTCC1700.11546638230239967No Hit
CATCAAGGGGTACATTTGGACGCTGTCCTGCGCTCCAGCTGCAAGCTGGGCT1700.11546638230239967No Hit
CAACACGGGGTACTGATCATGGAGTTAATCAACAATGTCGCCAAAGCCCATG1690.11478716828885614No Hit
AACTGTGGGATAGGTCCTTACAAAACCATTTAAAAATGTGAAGACTATTCTT1680.1141079542753126No Hit
TCATGCGGGATATTAGCACTGTTTGTAAACACCTGGTATGATAGCAATTTTT1670.11342874026176908No Hit
TGTTATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1670.11342874026176908No Hit
TCGCCAGGGATATTATTGATTTTTAATTTTATATAAGCAATTTTTTATTTCC1660.11274952624822557No Hit
GGTAACGGGGGGGCTATGTATTTAGCAAGAAACTCTTTAAAGTTGAAATTTA1650.11207031223468202No Hit
GCTCATGGGGAAGCATGTGATCTCTGTGACCCACACCCTATTCATACACTCC1650.11207031223468202No Hit
TTGCGTGGGAAGGCTACTGCAGGTCACTGAGTTGCCTGCCATCCAAAGGGGA1630.11071188420759498No Hit
TGCTATGGGATTTTACACATGAAGCAATTGAGTCCCAGAGAGGTAGAGTGAT1630.11071188420759498No Hit
CAAGCTGGGTAGATAAGAAGGTTGAGAAGGTGACAATCTCCAATAGACTTGT1620.11003267019405144No Hit
GTCTCAGGGACTTTTTCACTATTATAAATAATGCTGCTATGACCTTTCTCAT1610.10935345618050792No Hit
TAATAGAGGGTGGAATTAAAAGAGAAGGAAAAACACAAGGATGAAGTCTTTC1610.10935345618050792No Hit
AAGGAAGGGGTTGTAGAATTAGTGACATGAAAGTATGTATTAGTATCTTCAG1610.10935345618050792No Hit
TGGTTTGGGAAAGTGAAATTTAAATAGATAATTATAGGTTGATTACAAAATC1610.10935345618050792No Hit
GCGCACGGGGAGGCTTGCTGCGTGGAGATGGAAATCAATACGGGAACTGATA1590.10799502815342085No Hit
GCCAGGGGGAATGCACTTTCCTAACAGTGAATTCACAACCCTGAACAGCAGC1580.10731581413987734No Hit
ATGTGCGGGGTGGCCCCCCAGGACTGCGGCTGGCCGCCAGCTGGTGGGTGGA1580.10731581413987734No Hit
TCGTATGGGATTGCGTGTTAGAAGATTATTTAAATAATTGTTCTTTTGTTAT1570.1066366001263338No Hit
TCTAGTGGGATAATAAATTTGGAAGAAAAAGATTAAGACAGCTATAAAGAAA1570.1066366001263338No Hit
GAAACTGGGATGGTAAGTGTGTTTTTCTCCATTTCTATTTAGGCCAACACAC1550.10527817209924675No Hit
AAGAATGGGGTCTCCAAAAGAAAAGAAAAATGAAGTTTTAAAAATGAAACCA1540.10459895808570321No Hit
CGTTCAGGGAATATTGTACTGAAAGTCTGGCTGTATCATAAATATAACATTG1540.10459895808570321No Hit
AGCTACGGGAATGCCCTGTCTTCCAATATAATGACCTAATTTCTATTGATAT1530.1039197440721597No Hit
CTGCAGGGGGATTTTGATTCTTCCTCCTCTTTGCTGTCCTTTCAAGACACTT1530.1039197440721597No Hit
AATGTAGGGGCTATTGAAGAACTGTTTTATTTTGGCTAATTTGTGGTTTTAG1520.10324053005861618No Hit
GCATATGGGAAAAGAAAACTAACTGGAAAACAGGTTAGATTAATTCAGTACT1520.10324053005861618No Hit
CATCAAGGGGATATTTTAACATCTACTTTCATGACAGCCATGGATACTGCAA1520.10324053005861618No Hit
ATAATGGGAAGATGGCTCACTGTCCCAAATGTCCCCATGGGATTTTAGTTTC1520.10324053005861618No Hit
AGCCATGGGGTTCAGCATTATTATGCAAAAATTCACTAGTTGAGATGGTTTG1510.10256131604507264No Hit
CACACTCATTCTCAACCCCCTGACAAAACACATAGCCTACCCCTTCCTTGTA1510.10256131604507264No Hit
TTCCATGGGAGGGGTTCTGTATGGCCCATAATATAAAAATATAGGCCATCTG1500.10188210203152911No Hit
GTAAAAGGGATAATACACTTGCCCTGCTCCCTCTACCCCTTACCCCACAACG1500.10188210203152911No Hit
GGTCATGGGCCATCTCTAAGGATGTGACAGAGAAGGATATAGTGGACTTACT1500.10188210203152911No Hit
ACTGCAGGGGAATGTGGGTAAGGAAACAGCTGCTCCAATTTCCTATGAATTA1500.10188210203152911No Hit
GATAGCGGGAAGGTTTTAATGTGAGATATTCCTGCAAACCATCCCATTTCTA1490.1012028880179856No Hit
TCGCAGGGTGGGCCATGAGAACTAGGGGAAAGGAGTTTGAATGTGTCTATTT1490.1012028880179856No Hit
CTTCAAGGGATCTACTGGTGCTACCAAGGCTGTAGGCAAAGTCATCCTGGAG1480.10052367400444205No Hit
CGCCTAGGGGAACACGAAGAGATGAAAATCCTGAGGGAAGTTCTTAGAAAAT1480.10052367400444205No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)