FastQCFastQC Report
Sat 31 Dec 2022
SRR5849869.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849869.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81115
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCCGTCT53446.588177279171546No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGC52286.445170437033841No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGC15981.9700425322073598No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCA5890.7261295691302472No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCTTATAC5580.68791222338655No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTATACAC4670.5757258213647292No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCT4630.5707945509461876No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGC4390.541206928434938No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGT4150.5116193059236886No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAT3720.45860814892436663No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTA3570.4401158848548357No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC2930.36121555815817047No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCC2250.2773839610429637No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC2240.2761511434383283No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGC2180.26875423781051594No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATG1930.23793379769463108No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTCTTATA1800.22190716883437098No Hit
CTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGCCCA1630.20094926955556924No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGCCGTCT1590.1960179991370277No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCC1420.175060099858226No Hit
ATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCG1360.1676631942304136No Hit
TGTCTCTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGA1170.14423965974234113No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAA1160.14300684213770573No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTC1160.14300684213770573No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGCCGTCT1090.13437711890525797No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTGTCTCTTATACA1060.1306786660913518No Hit
GAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC1000.12328176046353942No Hit
TCGTGGGGGTCTGGTAGAGTTTGCCATTTTCTCTTTCCTGATAGAGTACAGC930.11465203723109166No Hit
GGGGAAGTAGGGATAGATGGTTTTATTTTATTTTGTTGTTTAATTTCAGATA900.11095358441718549No Hit
GTTGCGGGCAGGTGTCTCCTCTATCTTAGGGGCCATCAATTTCATCACAACA900.11095358441718549No Hit
TTTGCCGGGCCTGCCGGGGATCCTGAAATCTGCATCCGCCGTGGTTTGTATT880.10848794920791469No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTGTCTCTT880.10848794920791469No Hit
TATTGTGGGAGTCACTGATGGAGTTGTTTAGGAGGTAATTAGTAAAATATAC870.10725513160327929No Hit
AAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCG860.1060223139986439No Hit
ATTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATC860.1060223139986439No Hit
CAACCAGGGGAGGCAGTACCGGAGGAGAAAGATGGCGGCTACCTTACTAGCT850.1047894963940085No Hit
GCGCGAGAGGGGTTAGAAAATAATTTCAAATCTTCTTCATCCTTGTCTGTCT830.10232386118473771No Hit
ACTTTAGGGGGGGCAGAGAATAAAACAAGTGACAAAGCCAACAACAACCGGG820.10109104358010232No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)