FastQCFastQC Report
Sat 31 Dec 2022
SRR5849868.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849868.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80413
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGC54426.767562458806411No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCCGTCT53316.629525076791067No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGC15571.9362540882693096No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCA5540.6889433300585726No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCTTATAC5230.6503923494957282No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTATACAC5230.6503923494957282No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCT4630.5757775484063522No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGC4010.4986755872806636No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGT3890.4837526270627884No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTA3810.4738039869175382No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAT3640.45266312660888164No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC3120.3879969656647557No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCC2310.28726698419409796No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC2050.254933903722035No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTCTTATA1860.2313058833770659No Hit
CTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGCCCA1770.2201136632136595No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATG1740.21638292315919067No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGC1500.18653700272344023No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCC1490.18529342270528398No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGCCGTCT1410.17534478256003383No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTC1320.1641525623966274No Hit
ATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCG1300.16166540236031487No Hit
GTTGCGGGCAGGTGTCTCCTCTATCTTAGGGGCCATCAATTTCATCACAACA1190.14798602216059592No Hit
TCGTGGGGGTCTGGTAGAGTTTGCCATTTTCTCTTTCCTGATAGAGTACAGC1060.13181948192456444No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGCCGTCT1050.13057590190640816No Hit
TGTCTCTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGA980.1218708417793143No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTGTCTCTTATACA980.1218708417793143No Hit
TATTGTGGGAGTCACTGATGGAGTTGTTTAGGAGGTAATTAGTAAAATATAC970.12062726176115801No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTAT930.11565294168853295No Hit
GAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC930.11565294168853295No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAA920.11440936167037667No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTAT900.11192220163406415No Hit
AAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCG890.11067862161590787No Hit
ACTTTAGGGGGGGCAGAGAATAAAACAAGTGACAAAGCCAACAACAACCGGG880.10943504159775161No Hit
TATGCAGGGCAGAGGCTGGCCGGAAGCCCTGGGAGCGGAGCAACTCGGTGGA850.10570430154328281No Hit
ATTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATC850.10570430154328281No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)