FastQCFastQC Report
Sat 31 Dec 2022
SRR5849867.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849867.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80179
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCCGTCT54146.7523915239651275No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGC50436.289676848052482No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGC15351.9144663814714575No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCA5470.6822235248631188No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCTTATAC5190.6473016625300889No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGC4700.5861884034472866No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCT4600.5737163097569189No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTATACAC4370.545030494269073No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGT4100.5113558413050799No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAT4000.4988837476147121No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTA3410.425298394841542No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC2810.35046583269933523No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC2030.25318350191446637No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATG1870.2332281520098779No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCC1860.23198094264084113No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTCTTATA1760.21950884895047332No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGC1510.1883286147245538No Hit
CTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGCCCA1480.18458698661744347No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGCCGTCT1450.18084535851033312No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCC1440.17959814914129635No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTC1430.17835093977225958No Hit
TGTCTCTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGA1260.1571483804986343No Hit
ATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCG1250.1559011711295975No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTGTCTCTTATACA1180.14717070554634007No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGCCGTCT1140.14218186807019292No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAA1040.12970977437982514No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTAT1030.12846256501078837No Hit
AAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCG1010.1259681462727148No Hit
TCGTGGGGGTCTGGTAGAGTTTGCCATTTTCTCTTTCCTGATAGAGTACAGC1010.1259681462727148No Hit
CAACCAGGGGAGGCAGTACCGGAGGAGAAAGATGGCGGCTACCTTACTAGCT1000.12472093690367803No Hit
GTTGCGGGCAGGTGTCTCCTCTATCTTAGGGGCCATCAATTTCATCACAACA960.1197320994275309No Hit
ACTTTAGGGGGGGCAGAGAATAAAACAAGTGACAAAGCCAACAACAACCGGG950.11848489005849411No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTGTCTCTT930.11599047132042056No Hit
GGGGAAGTAGGGATAGATGGTTTTATTTTATTTTGTTGTTTAATTTCAGATA910.113496052582347No Hit
GAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC900.11224884321331022No Hit
ATTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATC870.10850721510619987No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTAT860.1072600057371631No Hit
ACCAAAGGGCAGAAGGGAGAGAAGATTCCTCGGTGCTTGCCAGTGTGTGGGA850.10601279636812631No Hit
TTTGCCGGGCCTGCCGGGGATCCTGAAATCTGCATCCGCCGTGGTTTGTATT840.10476558699908953No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)