FastQCFastQC Report
Sat 31 Dec 2022
SRR5849866.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849866.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30514
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGC20116.590417513272596No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCCGTCT13824.529068624238055No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGC3891.2748246706429835No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCA2450.8029101396080488No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCT1470.48174608376482925No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGC1420.4653601625483385No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAT1360.4456970570885495No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTA1310.42931113587205877No Hit
TTGTTGGGGCGATCTGCATGCGGGTGTCCAGGAATTGGGAAGAGTGAGAGGA1220.3998164776823753No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTATACAC1070.35065871403290294No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCTTATAC1020.33427279281641215No Hit
AAAACAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC990.32444124008651765No Hit
AGCACGGGGAATTTTCGATTTGACAGATGTAAGAAGCAGCATGAATAGTTTA960.3146096873566232No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGC950.311332503113325No Hit
TTTGCGGGATTCCTTGCATCTTTGGGTCTACTGAGCAGTGGGTGCTGAGGTG930.3047781346267287No Hit
TAGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAG910.2982237661401324No Hit
GTAATGGGGATTAGAAAACATTTAGTAAAAATCATCTCTAGTTCAAACCCTC910.2982237661401324No Hit
AATCGCGGGAACAATTAGAAAGTAATTGGAGCTTGAAGTATTCTAGAAGACT860.2818378449236416No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGT850.27856066068034346No Hit
CGCTCTGGGATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCAA830.2720062921937471No Hit
CATAATGGGAATTCAGTCCACCAAAGATTGAATTTTCTTAGACTATGTATAA800.26217473946385267No Hit
TTTGTTGGGAAGTTTATTTGGGGACAGAAATGGCAACTTGGACCCAAAGAAA790.2588975552205545No Hit
GCCACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAAGCTATCGGG790.2588975552205545No Hit
TATTCAGGGATAAAACATCCATATAGGATTATAGATACTTAAAGGAACACGT790.2588975552205545No Hit
AACGATGGGTCCAGCAATCTTTAGACTACAATAATACTTTTATCCATGTGCT760.24906600249066002No Hit
AAGCAGGGGCGACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCG730.23923444976076555No Hit
AATGAGGGGAGGGACAAGGTGTCATCCAAGAATTCACTTGAGTCCTATGCCT730.23923444976076555No Hit
AACTAGGGGATTCTCAGGAGGTCTGAGTTACCACTTTAAGATAGCAGAAAAA720.2359572655174674No Hit
ATTTGCGGGCACAACTGAAAACATGAGGGACTATATCCAGTCACTTGTAGCC710.23268008127416925No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCC670.21957134430097663No Hit
CTATTTGGGGTTTTTGCAGTATTCTATATATAATTTCCTTAATTTGTAAATT660.21629416005767843No Hit
TCATAAGGGAGTCTTAAGTAATGTGAATTCACTGTATTTCTTTTTATACAAC660.21629416005767843No Hit
ACTCTCGGGATTCCATGTGCTTAAATGATAATTTCCTTATTCAGTTTCAGAA660.21629416005767843No Hit
TATAAAGGGAGGGCAGGAACCCCACAGACTGTCCCTTCCAGCCCACACTCTG630.20646260732778396No Hit
CTCATGAAGGGAAGGACATGAATAGATGGTCTCCTTTAAGACTTGTTAAACA620.2031854230844858No Hit
AGCCAAGGATTTGAGATCAGCCTGGGCAACACAGGAAGACCCCCTCTCTACA620.2031854230844858No Hit
CTATAAGGGGTTAGTAGCCAAGCAAATGCAGTACATACTCATAAGACAGAAT600.19663105459788952No Hit
ATGTTAGGGTGACACGATTTCCTGCACGCGTCCTCCTCTACCTTGGAAGCAG600.19663105459788952No Hit
GTAAAAGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA580.19007668611129316No Hit
TACAATGGGCCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACAA550.1802451333813987No Hit
GCCTGGAGATTGTGAACCCACAGGCGGCAGAGAAGAAGGTGGCTGAGGCCAA540.17696794913810054No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC530.17369076489480237No Hit
ATCGCTGGGACCACAAAATGATGGAGCCAGAATTTAGTTGCAGAACTTCTGA520.17041358065150422No Hit
GCCTAGGGGGTGCTTAGTGATTGGTTTCCAAAGAGTAGAGTATTGAAAGTGG510.16713639640820607No Hit
TATCATGGGATCTTTCTTTTTACTTCAAAACACCAGATCCTAGTTGACACTG510.16713639640820607No Hit
TGGTAAGGGACACAATCAAAGACGCCCTCGGCTTACTTCTCTTCCTTCTCTC500.16385921216490792No Hit
TTTTCAGGGATCCTTAGATCTTTGCAAATTTCCAAATTACTCAATACCACCA500.16385921216490792No Hit
TGTTCTGGGCGATCTAGTTATGGGAATAGAAGGAAGTGCTAAAATCAAGTTA500.16385921216490792No Hit
TATGTTGGGGTGGCAGAGGACAGATATTAGAAATACTTTTTGGTTACAGAAA500.16385921216490792No Hit
TCATCCTGGGAACATCAGATCTTTTCTATAATATCCTACTACTTTGGTTTTC490.16058202792160975No Hit
ATTTTTGGGATGAAAAGAGTATTTAACTAGCCTAAATGTAGGTAGAACTATA490.16058202792160975No Hit
ATAAATGGGATATTCTGACTTGGCTATTGAGGGGAAAATTCAGTATTAAGTG480.1573048436783116No Hit
TGATATGGGGCGGCAAAGAAGCCAGAAGGAACCAGAATCCCAGTGCCCTACA480.1573048436783116No Hit
TGTCTAGGGTAGGTACAACATGGGAAAAGAATTCCCAACAAATTACACGTTT470.15402765943501343No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCC470.15402765943501343No Hit
TCATGAGGGGATAACCATGCACACTACTATAACCACCCTAACCCTGACTTCC470.15402765943501343No Hit
TATGGGGAACTGACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCA470.15402765943501343No Hit
TCTTGGGGGAAACTGAATAGTTGGTTCCTGAAATCTTGGACTGTTTATGACC470.15402765943501343No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGCCGTCT460.15075047519171528No Hit
CGTAGCGGGGATCTACAACCGGGACACAAAACTCTGTAAATCATTATAGCTT460.15075047519171528No Hit
GTTCTAGGGCAGTAAGTGCATATCCTAACTTGCCCATATTCTAAATCATCTG460.15075047519171528No Hit
TGAAATGGGATCCCAAGCTGCATGTCAAAGAAATTCAATTTCTAATCCGTAA460.15075047519171528No Hit
TACAAAGGGATGTGTAGTTTGCTTTCTTCATTTTTTTTCTTTTAAAATGCTC450.14747329094841713No Hit
GCAATGGGGAACAATTGTTGAATGAATAATTTGAATAATTGATTTGTCCCTG450.14747329094841713No Hit
TGCCAAGGGTCCCACCGGGGACTGGACACAAACAGGAATGTCTGGAGTGGTA450.14747329094841713No Hit
GCGAAAGGGGAAAACACAGAAGAAAAAAATACATAAGAAAATTGAAGTCACC450.14747329094841713No Hit
TGTGCCGGGAACTGGCCAAGTGTTAAGTGATGTGCTTATTGAGAGCAACTCC450.14747329094841713No Hit
ATAATGGGGGGAGTGCAGTAGCACGATCTCGGCTCTCACCGCAACCTCCGTC440.14419610670511895No Hit
GGGGCGGGACATAAACTTAGTTAATAAAGCAGCAGCAGTGTTTTCAATGTTG430.1409189224618208No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAA430.1409189224618208No Hit
CACGTGGGGGAACTATTTAACATATAAATGGGGACACAAAACCTGAATTGTG430.1409189224618208No Hit
ATCGCTGGGGGGGGCCCAGGTTGGATGGGGAAAGGCTAATGGGGTCAGACCC430.1409189224618208No Hit
CTGCGTTGGGGCAGGTTATCGAAATAACAATGTTAAGGAAACTCTTATCTCA420.13764173821852263No Hit
ATTGCTGGGCTATTCTGCCTAGCAAACTCAAACTACGAACGCACTCACAGTC420.13764173821852263No Hit
CTTTTAGGGGAAGCTTTGCTCATTCAAACAGATGAAGGCAAGAGTATTGCTA400.13108736973192633No Hit
TGTCGCGGGATTCTAGGAAAGACTAGGGATTCATTCTTTTATTTATTCTTTT400.13108736973192633No Hit
TATTTTGGGGAAGTTGCATTATTGTGGACTTGGCTGTCTTGTGATGAATTTT400.13108736973192633No Hit
ACTTATGGGGTATGGAAGCCACCGGGAGATTTCTGGATGGTGGTGCATCCGT390.1278101854886282No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGT390.1278101854886282No Hit
GTCGCAGGGCTCTCCCCTCTGACCCCTAGAAGGGGAGTTGTAGCCCCATGAA380.12453300124533001No Hit
TATAAAGGGGGAACAGAATAGAGAACCCAGAAATAAAGTCAGATACTTACAG380.12453300124533001No Hit
CAATAAGGGGCATCCAATTTTACACTCAGTTAAAGACCATTACTTCTCAGTG370.12125581700203185No Hit
GACCAAGGGGTCAGGGTCTCCTAATATTTATCTCAATTCTTTTATAAGTCTA370.12125581700203185No Hit
ATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCG370.12125581700203185No Hit
GTAATGGGGAGAGATTATAATATTATAATTGTAACACAGTTAAATATTATAT360.1179786327587337No Hit
CTTACAGGCATAAGCCACTGCACCTGGCCTCTTTTTGAATTTTTAAAATGCT360.1179786327587337No Hit
TCAGCAGGGTGGGGGGTGTTGCCCCATCACTGCCCAGCTCCTCTGACTGCCC360.1179786327587337No Hit
GCCTATGGGAACAGATACAGCTCGGAGTTGTGAGCACTCTACTGCAAGACTT360.1179786327587337No Hit
TATTTCGGGGGACGTACTTTGTCTGACTACAATATTCAAAAGGAGTCTACTC350.11470144851543553No Hit
CTCATTGGGACAGGAACACTACAAGATGGGACTGTTTTTGATACTAATATTC350.11470144851543553No Hit
TTGTAGGGTAAGAACCTGGGCTTCCAGATGCTGCAGAAGATGGGCTGGAAGG340.11142426427213738No Hit
CAATCAGGGAACTCTTCCTTGTAGGGCCCTTTCCTTATTCATTTAGGTAGTG340.11142426427213738No Hit
TTGTACGGGATTCTATTTGGTATTATATTATTTGATGTTTGCTGTTCTTCAA340.11142426427213738No Hit
AAGTTTGGGCATAGAAAAAGTATAAAGCTCAGTTAGTTTTTTTATTATTATT330.10814708002883922No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTCTTATA320.10486989578554107No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTC320.10486989578554107No Hit
GCAAATGGGATGGCATTGAGAATGAAGTCTTCCTGAGCCTTCCATGTATCCT320.10486989578554107No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATG320.10486989578554107No Hit
TGTCTCTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGA310.1015927115422429No Hit
CGCCGGGGGTTCACCAGCTTGTCTGCCTGCAGGGAGCCTGGTTTGGCTATCT310.1015927115422429No Hit
GCTATCGGGGACACAGCAGAAGGAAGAGAAAACCACAGGCAAGCCCAGGGGT310.1015927115422429No Hit
TTCAAGGGGAACTCTGAAAAAAAATCACATCATTGCTTGGCTTTTTCTGTTG310.1015927115422429No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)