FastQCFastQC Report
Sat 31 Dec 2022
SRR5849864.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849864.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30186
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGC20286.718346253229974No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCCGTCT13014.309945007619427No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGC3771.2489233419465977No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCA2170.7188763002716491No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGC2010.6658715961041543No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCT1710.5664877757901015No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAT1560.516795865633075No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTA1130.37434572318293247No Hit
TTGTTGGGGCGATCTGCATGCGGGTGTCCAGGAATTGGGAAGAGTGAGAGGA1110.36772013516199564No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCTTATAC1100.3644073411515272No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTATACAC980.32465381302590607No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCC940.31140263698403237No Hit
GTAATGGGGATTAGAAAACATTTAGTAAAAATCATCTCTAGTTCAAACCCTC940.31140263698403237No Hit
TTTGCGGGATTCCTTGCATCTTTGGGTCTACTGAGCAGTGGGTGCTGAGGTG910.3014642549526271No Hit
GCCACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAAGCTATCGGG910.3014642549526271No Hit
TAGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAG890.2948386669316902No Hit
AGCACGGGGAATTTTCGATTTGACAGATGTAAGAAGCAGCATGAATAGTTTA840.278274696879348No Hit
TCATAAGGGAGTCTTAAGTAATGTGAATTCACTGTATTTCTTTTTATACAAC830.2749619028688796No Hit
AACGATGGGTCCAGCAATCTTTAGACTACAATAATACTTTTATCCATGTGCT800.2650235208374743No Hit
AAAACAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC790.2617107268270059No Hit
AAGCAGGGGCGACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCG780.2583979328165375No Hit
GCCTAGGGGGTGCTTAGTGATTGGTTTCCAAAGAGTAGAGTATTGAAAGTGG780.2583979328165375No Hit
TACAATGGGCCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACAA780.2583979328165375No Hit
GTAAAAGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA770.25508513880606903No Hit
TATTCAGGGATAAAACATCCATATAGGATTATAGATACTTAAAGGAACACGT750.24845955078513218No Hit
CATAATGGGAATTCAGTCCACCAAAGATTGAATTTTCTTAGACTATGTATAA750.24845955078513218No Hit
ATTTGCGGGCACAACTGAAAACATGAGGGACTATATCCAGTCACTTGTAGCC720.2385211687537269No Hit
TTTGTTGGGAAGTTTATTTGGGGACAGAAATGGCAACTTGGACCCAAAGAAA710.23520837474325845No Hit
AATCGCGGGAACAATTAGAAAGTAATTGGAGCTTGAAGTATTCTAGAAGACT710.23520837474325845No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGT700.23189558073279004No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCC690.22858278672232163No Hit
TATAAAGGGAGGGCAGGAACCCCACAGACTGTCCCTTCCAGCCCACACTCTG690.22858278672232163No Hit
CGCTCTGGGATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCAA690.22858278672232163No Hit
AATGAGGGGAGGGACAAGGTGTCATCCAAGAATTCACTTGAGTCCTATGCCT690.22858278672232163No Hit
CTATTTGGGGTTTTTGCAGTATTCTATATATAATTTCCTTAATTTGTAAATT680.22526999271185316No Hit
AACTAGGGGATTCTCAGGAGGTCTGAGTTACCACTTTAAGATAGCAGAAAAA680.22526999271185316No Hit
AGCCAAGGATTTGAGATCAGCCTGGGCAACACAGGAAGACCCCCTCTCTACA680.22526999271185316No Hit
CTATAAGGGGTTAGTAGCCAAGCAAATGCAGTACATACTCATAAGACAGAAT680.22526999271185316No Hit
TGTTCTGGGCGATCTAGTTATGGGAATAGAAGGAAGTGCTAAAATCAAGTTA660.21864440469091634No Hit
ATTGCTGGGCTATTCTGCCTAGCAAACTCAAACTACGAACGCACTCACAGTC660.21864440469091634No Hit
ATCGCTGGGGGGGGCCCAGGTTGGATGGGGAAAGGCTAATGGGGTCAGACCC620.2053932286490426No Hit
ACTCTCGGGATTCCATGTGCTTAAATGATAATTTCCTTATTCAGTTTCAGAA610.2020804346385742No Hit
TCATCCTGGGAACATCAGATCTTTTCTATAATATCCTACTACTTTGGTTTTC600.19876764062810573No Hit
ATCGCTGGGACCACAAAATGATGGAGCCAGAATTTAGTTGCAGAACTTCTGA590.19545484661763732No Hit
GCCTGGAGATTGTGAACCCACAGGCGGCAGAGAAGAAGGTGGCTGAGGCCAA580.19214205260716888No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGC580.19214205260716888No Hit
CTCATGAAGGGAAGGACATGAATAGATGGTCTCCTTTAAGACTTGTTAAACA560.18551646458623203No Hit
TGTCGCGGGATTCTAGGAAAGACTAGGGATTCATTCTTTTATTTATTCTTTT550.1822036705757636No Hit
TATGTTGGGGTGGCAGAGGACAGATATTAGAAATACTTTTTGGTTACAGAAA530.17557808255482674No Hit
TTTTCAGGGATCCTTAGATCTTTGCAAATTTCCAAATTACTCAATACCACCA520.17226528854435832No Hit
TATAAAGGGGGAACAGAATAGAGAACCCAGAAATAAAGTCAGATACTTACAG520.17226528854435832No Hit
TGCCAAGGGTCCCACCGGGGACTGGACACAAACAGGAATGTCTGGAGTGGTA520.17226528854435832No Hit
GCTGCTGGGATTGCTAAGTGAAGTATGTCCTGTTTTCTGCTATAATTCTTTC510.16895249453388989No Hit
ATTTTTGGGATGAAAAGAGTATTTAACTAGCCTAAATGTAGGTAGAACTATA510.16895249453388989No Hit
CAATCAGGGAACTCTTCCTTGTAGGGCCCTTTCCTTATTCATTTAGGTAGTG510.16895249453388989No Hit
TCAGCAGGGTGGGGGGTGTTGCCCCATCACTGCCCAGCTCCTCTGACTGCCC510.16895249453388989No Hit
CACGTGGGGGAACTATTTAACATATAAATGGGGACACAAAACCTGAATTGTG510.16895249453388989No Hit
TTGTAGGGTAAGAACCTGGGCTTCCAGATGCTGCAGAAGATGGGCTGGAAGG490.16232690651295303No Hit
TCATGAGGGGATAACCATGCACACTACTATAACCACCCTAACCCTGACTTCC490.16232690651295303No Hit
TGTCTAGGGTAGGTACAACATGGGAAAAGAATTCCCAACAAATTACACGTTT480.1590141125024846No Hit
ATGTTAGGGTGACACGATTTCCTGCACGCGTCCTCCTCTACCTTGGAAGCAG480.1590141125024846No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAA470.15570131849201618No Hit
TCTTGGGGGAAACTGAATAGTTGGTTCCTGAAATCTTGGACTGTTTATGACC450.1490757304710793No Hit
CGTAGCGGGGATCTACAACCGGGACACAAAACTCTGTAAATCATTATAGCTT440.14576293646061086No Hit
TATCATGGGATCTTTCTTTTTACTTCAAAACACCAGATCCTAGTTGACACTG440.14576293646061086No Hit
ATAAATGGGATATTCTGACTTGGCTATTGAGGGGAAAATTCAGTATTAAGTG440.14576293646061086No Hit
AGTTTAGGGATCTGTTATTTTTGTAAACTCAAAGTTAAGATGATCAAAGTTC430.14245014245014245No Hit
TGGTAAGGGACACAATCAAAGACGCCCTCGGCTTACTTCTCTTCCTTCTCTC420.139137348439674No Hit
TACAAAGGGATGTGTAGTTTGCTTTCTTCATTTTTTTTCTTTTAAAATGCTC420.139137348439674No Hit
GCAAATGGGATGGCATTGAGAATGAAGTCTTCCTGAGCCTTCCATGTATCCT420.139137348439674No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGT420.139137348439674No Hit
ACGGCTGGGAACTTTGAGTCAGCTGGTAAATATGTATATCTTGTGTTAAAAA410.1358245544292056No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTAT410.1358245544292056No Hit
GCAATGGGGAACAATTGTTGAATGAATAATTTGAATAATTGATTTGTCCCTG410.1358245544292056No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTGTCTCTTATACA400.13251176041873716No Hit
CTTTTAGGGGAAGCTTTGCTCATTCAAACAGATGAAGGCAAGAGTATTGCTA400.13251176041873716No Hit
GTAATGGGGAGAGATTATAATATTATAATTGTAACACAGTTAAATATTATAT400.13251176041873716No Hit
GTTCTAGGGCAGTAAGTGCATATCCTAACTTGCCCATATTCTAAATCATCTG400.13251176041873716No Hit
GTCGCAGGGCTCTCCCCTCTGACCCCTAGAAGGGGAGTTGTAGCCCCATGAA390.12919896640826875No Hit
CTCATTGGGACAGGAACACTACAAGATGGGACTGTTTTTGATACTAATATTC390.12919896640826875No Hit
TGTGCCGGGAACTGGCCAAGTGTTAAGTGATGTGCTTATTGAGAGCAACTCC390.12919896640826875No Hit
TGATGGGGGACGACCTGATTTCCTTAGGACGGAACCGCAGGCACCTGCGCCG380.1258861723978003No Hit
ATAATGGGGGGAGTGCAGTAGCACGATCTCGGCTCTCACCGCAACCTCCGTC380.1258861723978003No Hit
AAGTTTGGGCATAGAAAAAGTATAAAGCTCAGTTAGTTTTTTTATTATTATT380.1258861723978003No Hit
TGAAATGGGATCCCAAGCTGCATGTCAAAGAAATTCAATTTCTAATCCGTAA370.12257337838733189No Hit
ATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCG360.11926058437686345No Hit
GCGAATGGGATTCAGGACCGGCACTTCTTAATGTTTGTGGCTTTCTCTCCAG360.11926058437686345No Hit
TGTCTCTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGA350.11594779036639502No Hit
TATTTCGGGGGACGTACTTTGTCTGACTACAATATTCAAAAGGAGTCTACTC340.11263499635592658No Hit
GGGGCGGGACATAAACTTAGTTAATAAAGCAGCAGCAGTGTTTTCAATGTTG340.11263499635592658No Hit
CTTACAGGCATAAGCCACTGCACCTGGCCTCTTTTTGAATTTTTAAAATGCT340.11263499635592658No Hit
CTGCGTTGGGGCAGGTTATCGAAATAACAATGTTAAGGAAACTCTTATCTCA330.10932220234545817No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC330.10932220234545817No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTCTTATA330.10932220234545817No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATG330.10932220234545817No Hit
ACTTATGGGGTATGGAAGCCACCGGGAGATTTCTGGATGGTGGTGCATCCGT320.10600940833498972No Hit
GACACTGGGAGCGGTTGACATCTGGAGACTATTTACAAGGATGTTCTTAATT320.10600940833498972No Hit
TGTTCTGGGAGGAATGGCTTATTCCAACTGTCAGCATGATTTCCAGGCTCCA320.10600940833498972No Hit
GGTACTGGGAGAGGTGCACTTGCATGGCAGGCTGGGCCCCCTTTTCTATATT320.10600940833498972No Hit
GACCAAGGGGTCAGGGTCTCCTAATATTTATCTCAATTCTTTTATAAGTCTA310.1026966143245213No Hit
GCTTCAGGGGATGGTGGAATTTGCATTGTCTAGTGCAAAATGCAGCCCTTTT310.1026966143245213No Hit
TATGGGGAACTGACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCA310.1026966143245213No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)