FastQCFastQC Report
Sat 31 Dec 2022
SRR5849863.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849863.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82106
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGC29923.6440698609115043No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCCGTCT28153.428494872481914No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGC5630.6856989744963827No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCTTATAC3170.38608627871288337No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCA3030.3690351496845541No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCT2560.3117920736608774No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGC2420.2947409446325482No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTATACAC2370.28865125569385913No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTA2150.2618566243636275No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAT2070.2521131220617251No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGT1690.20583148612768862No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCC1400.17051129028329234No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC1390.16929335249555455No Hit
AGGTAGGGAACAAGAAGAATTAATTCAACAGACTTTAGAACATGGTGTTTTG1260.15346016125496312No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGC1220.1485884101040119No Hit
TCTCATGGGATTGAAAGACATGGGAATTTATTTATGACAACTGTTTTAGGTG1070.13031934328794484No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGCCGTCT1040.12666552992473143No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCC970.11813996541056683No Hit
GTCTATGGGGTGGCTGTTGGATGAAGTAGTTTATAGGTGTCGACTGTACCTA930.11326821425961561No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTC930.11326821425961561No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTCTTATA900.1096144008964022No Hit
GGCATAGGGATTAAAGGAAACGTGGCAGGACTTCCATCTGTGCCATACTTGT900.1096144008964022No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC890.10839646310866441No Hit
TCCTTTGGGGGGCGCTCTCGCGCTAACTGTGCTCCTCCGGGGCCCTCCGCCT890.10839646310866441No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATG870.10596058753318881No Hit
AGCCTAGGGGCGATCTCAACAGGGGAGAAAAGAGAGATTTTTGGAAAGCACC860.104742649745451No Hit
ACAACAGGGGCATTTTACTTTTTTACTTAACAGTGGTGCCACATCAGTGCAT840.1023067741699754No Hit
AACAGCGGGAACAAGGAAGACTTCATACCTTACAAGGGGGGCCTGTTCCATT840.1023067741699754No Hit
ACTTAGAGGGATGAAACAAACAAAACCCCCTGGATATTTTATCTTTAGAAAG840.1023067741699754No Hit
TTAAGGGGGAAAAATGAAAACAAAGAGCTTTACAGAGAATTAAAGGAGAATT830.1010888363822376No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)