FastQCFastQC Report
Sat 31 Dec 2022
SRR5849861.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849861.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82066
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGC29263.5654229522579386No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCCGTCT27453.3448687641654278No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGC5620.6848146613701167No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCTTATAC3480.4240489362220652No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCA3070.3740891477591207No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGC2750.3350961421295055No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTATACAC2670.32534789072210174No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCT2520.3070699193332196No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTA2140.2607657251480516No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAT2070.25223600516657324No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGT2050.2497989423147223No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC1660.2022762167036288No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCC1360.16572027392586453No Hit
AGGTAGGGAACAAGAAGAATTAATTCAACAGACTTTAGAACATGGTGTTTTG1140.13891258255550412No Hit
TCTCATGGGATTGAAAGACATGGGAATTTATTTATGACAACTGTTTTAGGTG1100.1340384568518022No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTCTTATA990.120634611166622No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC980.11941607974069653No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGC980.11941607974069653No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTC980.11941607974069653No Hit
AGCCTAGGGGCGATCTCAACAGGGGAGAAAAGAGAGATTTTTGGAAAGCACC920.11210489118514368No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATG900.10966782833329272No Hit
AACAGCGGGAACAAGGAAGACTTCATACCTTACAAGGGGGGCCTGTTCCATT890.10844929690736725No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCC870.1060122340555163No Hit
GTCTACGGGGACATTTTATTTTAAATTTTTAATTTGAGGGGAAAAGAAATGT860.10479370262959083No Hit
TTAGCTGGGAAGACCATCACCCTGGAAGTGGAGCCCAGTGACACCATCGAAA830.1011381083518144No Hit
GGCGATGGGAGACACCTACTGTGTGCCAGGTCCAGTGGGGGAATTCTGAGAT830.1011381083518144No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)