FastQCFastQC Report
Sat 31 Dec 2022
SRR5849860.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849860.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46256
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGC39348.504842615012107No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCCGTCT23755.13446904185403No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGC4620.9987893462469734No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCTTATAC3400.7350397786233137No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGC2800.6053268765133172No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCT2710.5858699411968177No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTATACAC2420.5231753718436527No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCA2180.4712902109996541No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTA2170.4691283292978209No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGC1790.3869768246281563No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAT1410.3048253199584919No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGT1310.28320650294015914No Hit
CAGCTTGGGCAACAGACTGAGACCCCGTCTCGAAAGAAGAAAGAAAGAAAGA970.20970252507782772No Hit
CACAGGGCGATCTTCTAGCGGGATTGTTCTTATATTAAAACAAAAGAACCTA920.19889311656866135No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGCCGTCT890.19240747146316153No Hit
CAGATGGGGCTATTTCGACCGCGATGATGTGGCTCTGGAAGGCGTGAGCCAC860.18592182635766172No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCC840.18159806295399517No Hit
CAACAGGGGGGAGTGAGATCTCAGATTTCTCTCCATCCTTTTCCCATTAGGA810.17511241784849532No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCC800.17295053614666206No Hit
GCAGCAGGGATACCTGTGCTCCTTTTTCTTACTGTGTTTTCAGTCACTTCCT790.17078865444482877No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC790.17078865444482877No Hit
TCAGTGGGGATTCTTTCTGCTGATGACAAAACTGTTCGACTTTGGGATCATG790.17078865444482877No Hit
CGACTAGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC790.17078865444482877No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTC780.1686267727429955No Hit
CTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGCCCA780.1686267727429955No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATG780.1686267727429955No Hit
TGCTTAGGGGCGCAGGCGCGGTGCACTGCGCGCCGGCACGAGGGTGTTTTCG770.16646489104116224No Hit
GCGCGCGGGCTTTAGCTGATTTTTTAGCTATAAAAATGTATTTGAAAAGCTC770.16646489104116224No Hit
GACAAAAGGGATTCCAAGTGAAAATCATGCAGCTGAGTCCTGCTGCATCCTG750.16214112763749566No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTCTTATA740.1599792459356624No Hit
CGTAATGGGGAGTGGAGGAGGAATGCCTTCTCCTAAGAGTCAGTTGAAAGAA740.1599792459356624No Hit
TTAATGGGGTTTGTTGTCTGATCTGTTAAGTTACATGTATATATTCCATCTT720.15565548253199585No Hit
GCATGTGGGAATGTGTTCTATATGTAAACTACAAATTCTATGGTAGCTTTGT710.15349360083016256No Hit
AGAAGTGGGCAGCTTTTCCATTTTGCTCTGCTGCAGTAAATTGCAATTGCCA690.14916983742649603No Hit
CCAAGTGGGAAGAATGGACTTCATTAAATAGACAAAATATGAGAGCCATTAC680.14700795572466274No Hit
TGTCTCTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGA660.1426841923209962No Hit
AGACAAGGGACCAGGTATATGAAAAAGGAACTGGAAATTTTAATTGTCCAGA660.1426841923209962No Hit
GGATTAGGGATGGTTGAGTAACATAGAATGTCGGCTGTAGTTGTCCTTAACA640.13836042891732964No Hit
ATAGCTGGGATTTAATATGTTTGTTCTTAAATATTTCAGTAACTGAAATAAT620.13403666551366308No Hit
ATTCTAGGGCTCTTATCTTAGTCATGTGTACATTATTAACAAAGAAACTAAG620.13403666551366308No Hit
CACGCTGGGATACATTTTCTGTTACAATGCTTGGTCATTGCATCGAAATGTA620.13403666551366308No Hit
TCAATAGGGGTCCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAA620.13403666551366308No Hit
TATAGTGGGATAATAACAAGAGTCAGAACATTAATTCGAAACAACTTGCAGT610.13187478381182982No Hit
ATAGGTGGGAAAACAAGAAGAGAACTTTTCTAAGGAATGATACATAGAAAAG610.13187478381182982No Hit
CGTTTGGGGATGGGAAGGCAACTTGAAGTCATTGTATTTGATTGAAAATGTT590.12755102040816327No Hit
GCCGTAGGGACCAGGAGTTTCACTCCTAGGTATGTACTCAAAAGAACTGTGT580.12538913870632998No Hit
AATATTGGGGTTTCAGTTCCTTTGAGTGGAAGATTTATTTTTTGTTCATTTT570.12322725700449672No Hit
GATCGCGGGACACTCGGTCCTGTTTCCAGGTTTTCCTCACTGTTCATTTTGC560.12106537530266344No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGCCGTCT560.12106537530266344No Hit
CGACGAGGGACTGAAACACACTGGTGCTTTCATCATCAGAGGTCAAATTATT550.11890349360083015No Hit
AAGCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC540.11674161189899689No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC530.11457973019716361No Hit
CAAATAGGGATCCTCAAGGGCAAACTCTTTGATTCTACAATAGCTGATGAGG530.11457973019716361No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTGTCTCTTATACA530.11457973019716361No Hit
GCTAATGGGGGGCGGTGGGGACGGAGGGGCCAGGTAGATAATAGTTCTTAAC530.11457973019716361No Hit
TGTTCAGGGCAGCAGATGATGGGTAACAATTGCAGTTCTTTTTTGAGATAGT510.11025596679349707No Hit
AGCGTGGGGACAGAAACAGCAGACCGAGAGCTGCTTCCATCTTTCCATGAAG510.11025596679349707No Hit
TCTTTAGGGATAATTGGTTTCTGTATCGTTTTGTTTCAACTCATGTATTCAC510.11025596679349707No Hit
TATCGTGGGCTACTGCAATTCTGAAGTGTTCATTTCTCACAAACTGTACTGA500.10809408509166378No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTAT500.10809408509166378No Hit
ACATAAGGGTCTTGTAGCGGGGCCCAAATATTGATAAAAAGGAAGAAGATTT500.10809408509166378No Hit
GTTTAGGGGAGCCAAGAAATTCACCATGAAAAAACAAGAATAACAAATAGAA500.10809408509166378No Hit
ACTTGCGGGGTATTAATTTACTATGAAATGGACATCCTTTTAACTAAAATGG490.1059322033898305No Hit
ATATTTAATGCAGATGCATAGTGTTTTTCAAATCTTCAACATCATTTTTTCA470.10160843998616396No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)