FastQCFastQC Report
Sat 31 Dec 2022
SRR5849859.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849859.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45492
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGC38178.390486239338784No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCCGTCT22354.912951727776313No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGC4220.9276356282423284No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGC2990.6572584190626923No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCTTATAC2910.6396729095225534No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCT2720.5979073243647235No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTATACAC2240.49239426712388995No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTA2160.474808757583751No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCA1980.43524136111843836No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAT1680.3692957003429174No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGC1300.28576453002725755No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGT1140.2505935109469797No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCC970.21322430317418448No Hit
CAGATGGGGCTATTTCGACCGCGATGATGTGGCTCTGGAAGGCGTGAGCCAC940.20662973709663235No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTCTTATA920.20223335971159764No Hit
TCAGTGGGGATTCTTTCTGCTGATGACAAAACTGTTCGACTTTGGGATCATG920.20223335971159764No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCC830.18244966147894134No Hit
CAGCTTGGGCAACAGACTGAGACCCCGTCTCGAAAGAAGAAAGAAAGAAAGA830.18244966147894134No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTC810.17805328409390664No Hit
GCAGCAGGGATACCTGTGCTCCTTTTTCTTACTGTGTTTTCAGTCACTTCCT760.1670623406313198No Hit
GCGCGCGGGCTTTAGCTGATTTTTTAGCTATAAAAATGTATTTGAAAAGCTC760.1670623406313198No Hit
CACAGGGCGATCTTCTAGCGGGATTGTTCTTATATTAAAACAAAAGAACCTA740.16266596324628507No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC730.1604677745537677No Hit
TCAATAGGGGTCCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAA730.1604677745537677No Hit
GACAAAAGGGATTCCAAGTGAAAATCATGCAGCTGAGTCCTGCTGCATCCTG720.1582695858612503No Hit
GCATGTGGGAATGTGTTCTATATGTAAACTACAAATTCTATGGTAGCTTTGT720.1582695858612503No Hit
GTTGTTGGGGAATTAGGCTGCAGCTAAAGGGAATGTTGTGGCTGGTTTAATC700.1538732084762156No Hit
TTAATGGGGTTTGTTGTCTGATCTGTTAAGTTACATGTATATATTCCATCTT680.14947683109118087No Hit
ATTCTAGGGCTCTTATCTTAGTCATGTGTACATTATTAACAAAGAAACTAAG680.14947683109118087No Hit
GGATTAGGGATGGTTGAGTAACATAGAATGTCGGCTGTAGTTGTCCTTAACA670.1472786423986635No Hit
TGCTTAGGGGCGCAGGCGCGGTGCACTGCGCGCCGGCACGAGGGTGTTTTCG670.1472786423986635No Hit
CACGCTGGGATACATTTTCTGTTACAATGCTTGGTCATTGCATCGAAATGTA670.1472786423986635No Hit
CTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGCCCA650.14288226501362877No Hit
AGAAGTGGGCAGCTTTTCCATTTTGCTCTGCTGCAGTAAATTGCAATTGCCA640.1406840763211114No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTGTCTCTTATACA640.1406840763211114No Hit
CGTAATGGGGAGTGGAGGAGGAATGCCTTCTCCTAAGAGTCAGTTGAAAGAA640.1406840763211114No Hit
CAACAGGGGGGAGTGAGATCTCAGATTTCTCTCCATCCTTTTCCCATTAGGA630.13848588762859404No Hit
TAACTTGGGCAACTGGTAGTCCATTAAATTCTCCTGTCTAGAATGACCCCCC630.13848588762859404No Hit
CCAAGTGGGAAGAATGGACTTCATTAAATAGACAAAATATGAGAGCCATTAC630.13848588762859404No Hit
AGACAAGGGACCAGGTATATGAAAAAGGAACTGGAAATTTTAATTGTCCAGA610.1340895102435593No Hit
AATATTGGGGTTTCAGTTCCTTTGAGTGGAAGATTTATTTTTTGTTCATTTT590.12969313285852457No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATG590.12969313285852457No Hit
TGTTCAGGGCAGCAGATGATGGGTAACAATTGCAGTTCTTTTTTGAGATAGT580.1274949441660072No Hit
GATCGCGGGACACTCGGTCCTGTTTCCAGGTTTTCCTCACTGTTCATTTTGC580.1274949441660072No Hit
ATAGCTGGGATTTAATATGTTTGTTCTTAAATATTTCAGTAACTGAAATAAT570.12529675547348984No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGCCGTCT570.12529675547348984No Hit
TGTCTCTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGA560.12309856678097249No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC560.12309856678097249No Hit
TATAGTGGGATAATAACAAGAGTCAGAACATTAATTCGAAACAACTTGCAGT550.12090037808845512No Hit
TGCTAGGGGCACGGGAAAGAGGGAGTCATTTTATTCCAAGATGTTTTATCTT550.12090037808845512No Hit
CGACTAGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC550.12090037808845512No Hit
AGCGTGGGGACAGAAACAGCAGACCGAGAGCTGCTTCCATCTTTCCATGAAG550.12090037808845512No Hit
TACTGAGGGGATTCATACAGCCTTGAAAAAAGTAAAACCCTGACCTGTAACC540.11870218939593775No Hit
GTTCTAGGGGCATGGACCACCTTGGGATACCGCAGCACCATCAACATCCGCC540.11870218939593775No Hit
CGTTTGGGGATGGGAAGGCAACTTGAAGTCATTGTATTTGATTGAAAATGTT540.11870218939593775No Hit
AAGCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC540.11870218939593775No Hit
ATATTTAATGCAGATGCATAGTGTTTTTCAAATCTTCAACATCATTTTTTCA540.11870218939593775No Hit
GCTGCTTTTGCCCAGTTCGGTTCTGACCTCGATGCTGCCACTCAACAACTTT530.11650400070342037No Hit
CAAATAGGGATCCTCAAGGGCAAACTCTTTGATTCTACAATAGCTGATGAGG520.11430581201090301No Hit
GCCGTAGGGACCAGGAGTTTCACTCCTAGGTATGTACTCAAAAGAACTGTGT520.11430581201090301No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGCCGTCT520.11430581201090301No Hit
GCCGTACGGGAGGAATGAGATAAGAAAGGATATGATGGCAGGAAAAGAAATG520.11430581201090301No Hit
ACTTGCGGGGTATTAATTTACTATGAAATGGACATCCTTTTAACTAAAATGG510.11210762331838565No Hit
GCTAATGGGGGGCGGTGGGGACGGAGGGGCCAGGTAGATAATAGTTCTTAAC510.11210762331838565No Hit
CTGCGAGGGACCTTGACTGTAGTTGTCATATACTCTGAAAGAAATTTATATA500.10990943462586829No Hit
CCGTATGGGATAGCAGGTTTGGAATGCTGGCGAGTTGCACCTTTCAGGTGCA500.10990943462586829No Hit
TATCGTGGGCTACTGCAATTCTGAAGTGTTCATTTCTCACAAACTGTACTGA490.10771124593335092No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTAT490.10771124593335092No Hit
TAGCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC480.10551305724083357No Hit
CCGGAATAGGGGAATTTTTACCGAATATGAAATTTCCAAATTAAAAACGTAT480.10551305724083357No Hit
GCATTGGGGGTGTCTGCCTTCTTCAACATGTAGGGTTGATCAAGCTAATACT470.10331486854831617No Hit
CGTTGGGGGCAGAAGGAAATGGGCTATCACTACATTTAAGTGAAATACAACC470.10331486854831617No Hit
AGCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCG460.10111667985579882No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTGTCTCTT460.10111667985579882No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)