FastQCFastQC Report
Sat 31 Dec 2022
SRR5849858.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849858.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45312
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGC37008.165607344632768No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCCGTCT22104.877295197740112No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGC4150.9158721751412429No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGC3440.759180790960452No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCT2710.5980755649717514No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCTTATAC2690.5936617231638418No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTATACAC2310.5097987288135594No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCA2070.4568326271186441No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTA1840.4060734463276836No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAT1460.3222104519774011No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGT1180.26041666666666663No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGC1130.24938206214689265No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTCTTATA1050.23172669491525422No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCC980.2162782485875706No Hit
CAGCTTGGGCAACAGACTGAGACCCCGTCTCGAAAGAAGAAAGAAAGAAAGA920.2030367231638418No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATG910.20082980225988703No Hit
TCAGTGGGGATTCTTTCTGCTGATGACAAAACTGTTCGACTTTGGGATCATG860.189795197740113No Hit
CAGATGGGGCTATTTCGACCGCGATGATGTGGCTCTGGAAGGCGTGAGCCAC850.1875882768361582No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC850.1875882768361582No Hit
GACAAAAGGGATTCCAAGTGAAAATCATGCAGCTGAGTCCTGCTGCATCCTG840.1853813559322034No Hit
GCAGCAGGGATACCTGTGCTCCTTTTTCTTACTGTGTTTTCAGTCACTTCCT830.1831744350282486No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTC830.1831744350282486No Hit
CGTTTGGGGATGGGAAGGCAACTTGAAGTCATTGTATTTGATTGAAAATGTT810.178760593220339No Hit
TATAGTGGGATAATAACAAGAGTCAGAACATTAATTCGAAACAACTTGCAGT790.1743467514124294No Hit
TTAATGGGGTTTGTTGTCTGATCTGTTAAGTTACATGTATATATTCCATCTT780.17213983050847456No Hit
CTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGCCCA750.16551906779661016No Hit
CCAAGTGGGAAGAATGGACTTCATTAAATAGACAAAATATGAGAGCCATTAC720.15889830508474578No Hit
AGAAGTGGGCAGCTTTTCCATTTTGCTCTGCTGCAGTAAATTGCAATTGCCA710.15669138418079098No Hit
CACAGGGCGATCTTCTAGCGGGATTGTTCTTATATTAAAACAAAAGAACCTA700.15448446327683615No Hit
TGCTTAGGGGCGCAGGCGCGGTGCACTGCGCGCCGGCACGAGGGTGTTTTCG690.15227754237288135No Hit
GGATTAGGGATGGTTGAGTAACATAGAATGTCGGCTGTAGTTGTCCTTAACA680.15007062146892655No Hit
CGTAATGGGGAGTGGAGGAGGAATGCCTTCTCCTAAGAGTCAGTTGAAAGAA670.14786370056497175No Hit
AGACAAGGGACCAGGTATATGAAAAAGGAACTGGAAATTTTAATTGTCCAGA660.14565677966101695No Hit
GCTAATGGGGGGCGGTGGGGACGGAGGGGCCAGGTAGATAATAGTTCTTAAC650.14344985875706215No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC640.14124293785310735No Hit
CGACTAGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC640.14124293785310735No Hit
TACTGAGGGGATTCATACAGCCTTGAAAAAAGTAAAACCCTGACCTGTAACC630.13903601694915255No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCC630.13903601694915255No Hit
ATTCTAGGGCTCTTATCTTAGTCATGTGTACATTATTAACAAAGAAACTAAG610.13462217514124294No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTGTCTCTT610.13462217514124294No Hit
GATCGCGGGACACTCGGTCCTGTTTCCAGGTTTTCCTCACTGTTCATTTTGC610.13462217514124294No Hit
GCGCGCGGGCTTTAGCTGATTTTTTAGCTATAAAAATGTATTTGAAAAGCTC600.13241525423728812No Hit
CAAAGAGGGGTTTAGCTGTTCATCAGTTGATAGGTAGGCATTGAGTTGTTTT590.13020833333333331No Hit
ACTTGCGGGGTATTAATTTACTATGAAATGGACATCCTTTTAACTAAAATGG590.13020833333333331No Hit
CAACAGGGGGGAGTGAGATCTCAGATTTCTCTCCATCCTTTTCCCATTAGGA580.1280014124293785No Hit
TGTTCAGGGCAGCAGATGATGGGTAACAATTGCAGTTCTTTTTTGAGATAGT580.1280014124293785No Hit
GCCGTAGGGACCAGGAGTTTCACTCCTAGGTATGTACTCAAAAGAACTGTGT580.1280014124293785No Hit
GCATGTGGGAATGTGTTCTATATGTAAACTACAAATTCTATGGTAGCTTTGT570.1257944915254237No Hit
TATCGTGGGCTACTGCAATTCTGAAGTGTTCATTTCTCACAAACTGTACTGA560.12358757062146894No Hit
CACGCTGGGATACATTTTCTGTTACAATGCTTGGTCATTGCATCGAAATGTA560.12358757062146894No Hit
TCAATAGGGGTCCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAA560.12358757062146894No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGCCGTCT550.12138064971751414No Hit
ATAGGTGGGAAAACAAGAAGAGAACTTTTCTAAGGAATGATACATAGAAAAG550.12138064971751414No Hit
TGTCTCTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGA540.11917372881355932No Hit
CGTTGGGGGCAGAAGGAAATGGGCTATCACTACATTTAAGTGAAATACAACC540.11917372881355932No Hit
ATATTTAATGCAGATGCATAGTGTTTTTCAAATCTTCAACATCATTTTTTCA540.11917372881355932No Hit
GTTGTTGGGGAATTAGGCTGCAGCTAAAGGGAATGTTGTGGCTGGTTTAATC530.11696680790960452No Hit
AATATTGGGGTTTCAGTTCCTTTGAGTGGAAGATTTATTTTTTGTTCATTTT530.11696680790960452No Hit
TGCTAGGGGCACGGGAAAGAGGGAGTCATTTTATTCCAAGATGTTTTATCTT530.11696680790960452No Hit
GTTCTAGGGGCATGGACCACCTTGGGATACCGCAGCACCATCAACATCCGCC530.11696680790960452No Hit
ATATTAGGGCTAGGGGCTGTGGCTGTTTCCGGGGAGGCCGGGAGGGGCAGCT530.11696680790960452No Hit
ACATAAGGGTCTTGTAGCGGGGCCCAAATATTGATAAAAAGGAAGAAGATTT520.11475988700564972No Hit
CGACGAGGGACTGAAACACACTGGTGCTTTCATCATCAGAGGTCAAATTATT520.11475988700564972No Hit
CAAATAGGGATCCTCAAGGGCAAACTCTTTGATTCTACAATAGCTGATGAGG510.11255296610169492No Hit
TTACAAGGGAATTACAATTTAGGGTAACAACCAGGATGTATGGATTCTTGAG510.11255296610169492No Hit
ATGCCAGGGGTTTTTGAGTGTGAAAAGTTGAGTAAAACTATTTGCAACTGGT490.1081391242937853No Hit
TGGCCTGTTGTGGGGTGGGGGAAGCGGGGAGGGATAGCACTAGGAGATATAC490.1081391242937853No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGCCGTCT480.1059322033898305No Hit
TAACTTGGGCAACTGGTAGTCCATTAAATTCTCCTGTCTAGAATGACCCCCC480.1059322033898305No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTGTCTCTTATACA480.1059322033898305No Hit
CCGTATGGGATAGCAGGTTTGGAATGCTGGCGAGTTGCACCTTTCAGGTGCA480.1059322033898305No Hit
TCTTTAGGGATAATTGGTTTCTGTATCGTTTTGTTTCAACTCATGTATTCAC480.1059322033898305No Hit
AGCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCG470.1037252824858757No Hit
AGAGCTGGGAAGAAGAGATCGTGCCCTCATTTTTCACTTATTTTTTTGGTTA470.1037252824858757No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAA470.1037252824858757No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)