FastQCFastQC Report
Sat 31 Dec 2022
SRR5849857.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849857.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26659
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGC18797.048276379459094No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCCGTCT15635.86293559398327No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGC6792.546982257399002No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGT3991.4966802955849807No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCA2090.783975392925466No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCTTATAC1800.6751941183090139No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTATACAC1590.5964214711729622No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGT1510.5664128436925616No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCT1490.5589106868224615No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGC1370.5138977456018605No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCC1320.49514235342661017No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAT1290.48388911812145996No Hit
CGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCG1130.4238718631606587No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTA1130.4238718631606587No Hit
CGGTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCT1050.39386323568025805No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC960.3601035297648074No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATG820.3075884316741063No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGC720.2700776473236055No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTCTTATA700.26257549045350537No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC650.243820098278255No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTAT580.21756254923290447No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGCCGTCT530.19880715705765406No Hit
TGCATAGGGATTGCAAGGGGGGAAAAAGAAGAAAGAATGGGATTAGGGTGAT510.19130500018755392No Hit
ATCTAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCA480.1800517648824037No Hit
TGTCTCTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGA450.16879852957725347No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCC440.1650474511422034No Hit
ATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCG440.1650474511422034No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTC430.1612963727071533No Hit
CTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGCCCA410.15379421583705316No Hit
GTCGGGCCGATCTTGACATGGGTATTTATTCTAGCCACCTCTAGCCTAGCCG390.146292058966953No Hit
CGAGCAGGGCTGCAAGAGGGATCCCACCTGATCCCAGTCTGAAGGTCTCTTA390.146292058966953No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTGTCTCTTATACA380.14254098053190292No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGCCGTCT380.14254098053190292No Hit
ACTGCTGGGATGGATTCTTTCAGAAAACCTGATTTCAAATCAGAAATACTCA370.13878990209685285No Hit
CGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC370.13878990209685285No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTAT330.12378558835665254No Hit
GACTGAGGGGGGAGTGGCTTTTCCTCTGGTAGCCACACACCTGAGCACTACG330.12378558835665254No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAA330.12378558835665254No Hit
TTACTAGGGGCAGCCCGTGGAACACAGAGCATTCCTAATGACAGTCCTGCCC320.12003450992160244No Hit
CTCATCGGGGCTTTCCCTGCGTAGATCTAGGCCAGGGGCTGCTTGTTTTTGT310.11628343148655237No Hit
GAAGTGGGGGGGCCCTCTCAGCCCTCCTAATGACCTCCGGCCTAGCCATGTG300.1125323530515023No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTGTCTCTT290.10878127461645223No Hit
TTAGCTGGGACATCAAGACGAGAAGACCCTATGGAGCTTTAATTTATTAATG270.10127911774635208No Hit
TCACTTGGGAAGCCCTGCCCCTCCTGTACATATAGCCCTTTCCCCACTCAGC270.10127911774635208No Hit
ATTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATC270.10127911774635208No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)