FastQCFastQC Report
Sat 31 Dec 2022
SRR5849856.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849856.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26429
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGC17676.6858375269590224No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCCGTCT16546.258276892807143No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGC5872.221045064134095No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGT4311.6307843656589351No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCA2200.8324189337470204No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTATACAC1870.7075560936849672No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGT1850.6999886488327217No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCTTATAC1600.6053955881796511No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCT1550.586476976049037No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGC1540.5826932536229142No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCC1410.5335048620833176No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAT1340.5070188051004578No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTA1070.4048582995951417No Hit
CGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCG1040.3935071323167732No Hit
CGGTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCT1020.3859396874645276No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATG1000.378372242612282No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC830.314048961368194No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGC660.24972568012410612No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC630.23837451284573763No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTCTTATA620.2345907904196148No Hit
GTCGGGCCGATCTTGACATGGGTATTTATTCTAGCCACCTCTAGCCTAGCCG600.2270233455673692No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTAT520.1967535661583866No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTC500.189186121306141No Hit
CTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGCCCA500.189186121306141No Hit
ACTGCTGGGATGGATTCTTTCAGAAAACCTGATTTCAAATCAGAAATACTCA440.16648378674940406No Hit
ATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCG440.16648378674940406No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAA440.16648378674940406No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGCCGTCT440.16648378674940406No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTGTCTCTT420.15891634189715842No Hit
TGCATAGGGATTGCAAGGGGGGAAAAAGAAGAAAGAATGGGATTAGGGTGAT410.15513261947103563No Hit
GACTGAGGGGGGAGTGGCTTTTCCTCTGGTAGCCACACACCTGAGCACTACG410.15513261947103563No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGCCGTCT400.15134889704491278No Hit
TGTCTCTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGA390.14756517461878996No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCC380.14378145219266716No Hit
CGAGTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCC370.13999772976654432No Hit
TTACTAGGGGCAGCCCGTGGAACACAGAGCATTCCTAATGACAGTCCTGCCC370.13999772976654432No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTGTCTCTTATACA360.1362140073404215No Hit
TGTCTCTTATACACANCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGC340.12864656248817585No Hit
ATCTAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCA340.12864656248817585No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTAT310.1172953952098074No Hit
GCGTTTGGGGCATTACAAAGAACTCCAAGAAGCTTTCTAAATTCTTTTGATG310.1172953952098074No Hit
CGAGCAGGGCTGCAAGAGGGATCCCACCTGATCCCAGTCTGAAGGTCTCTTA300.1135116727836846No Hit
CACCGAGGGGTGAATTATAGAGGAAATACAGAGAGAACCTCTTCCACTTTTA290.10972795035756176No Hit
ATTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATC290.10972795035756176No Hit
CTCATCGGGGCTTTCCCTGCGTAGATCTAGGCCAGGGGCTGCTTGTTTTTGT290.10972795035756176No Hit
CTGCTCGGGGATGGGGTTCAGGTGAGAATCCTGGCTCTGGTGCTCAGTCCTG270.10216050550531613No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)