FastQCFastQC Report
Sat 31 Dec 2022
SRR5849855.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849855.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26537
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGC17326.526736255040133No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCCGTCT16506.217733730263405No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGC6202.336360553189886No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGT4451.6769039454346761No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCA1980.7461280476316087No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCT1680.6330783434450012No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAT1630.6142367260805667No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGC1600.602931755661906No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCTTATAC1420.5351019331499416No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGT1400.5275652862041678No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTATACAC1330.5011870218939594No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCC1320.49741869842107245No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTA1280.4823454045295248No Hit
CGGTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCT1000.37683234728869125No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATG980.3692957003429174No Hit
CGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCG950.35799072992425673No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC820.30900252477672685No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGC610.22986773184610165No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCC580.21856276142744094No Hit
ATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCG580.21856276142744094No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC550.2072577910087802No Hit
TGCATAGGGATTGCAAGGGGGGAAAAAGAAGAAAGAATGGGATTAGGGTGAT510.19218449711723257No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTAT510.19218449711723257No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTC490.1846478501714587No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGCCGTCT470.17711120322568488No Hit
CTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGCCCA450.16957455627991108No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTCTTATA440.16580623280702417No Hit
CGAGCAGGGCTGCAAGAGGGATCCCACCTGATCCCAGTCTGAAGGTCTCTTA430.16203790933413725No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.1582695858612503No Hit
GACTGAGGGGGGAGTGGCTTTTCCTCTGGTAGCCACACACCTGAGCACTACG400.1507329389154765No Hit
GTCGGGCCGATCTTGACATGGGTATTTATTCTAGCCACCTCTAGCCTAGCCG380.14319629196970268No Hit
GCGTTTGGGGCATTACAAAGAACTCCAAGAAGCTTTCTAAATTCTTTTGATG360.13565964502392885No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTAT350.13189132155104194No Hit
ACTGCTGGGATGGATTCTTTCAGAAAACCTGATTTCAAATCAGAAATACTCA350.13189132155104194No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTGTCTCTTATACA350.13189132155104194No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTGTCTCTT350.13189132155104194No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGCCGTCT330.12435467460526811No Hit
TGTCTCTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGA320.1205863511323812No Hit
CACCGAGGGGTGAATTATAGAGGAAATACAGAGAGAACCTCTTCCACTTTTA320.1205863511323812No Hit
ATTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATC320.1205863511323812No Hit
TTGTCTGGGACACTCAGCTTGCAAAGCCTTCTCTAGAAAATAACCCACTCTG300.11304970418660738No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAA300.11304970418660738No Hit
TCTACCGGGGGAGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCAC300.11304970418660738No Hit
ATCTAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCA280.10551305724083357No Hit
GAAGTGGGGGGGCCCTCTCAGCCCTCCTAATGACCTCCGGCCTAGCCATGTG270.10174473376794663No Hit
CGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC270.10174473376794663No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)