FastQCFastQC Report
Sat 31 Dec 2022
SRR5849853.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849853.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48904
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGC44199.036070669065925No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCCGTCT39017.9768526091935215No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGC7301.4927204318665142No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCA5341.091935220022902No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGC3130.6400294454441354No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAT2670.545967610011451No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCTTATAC2400.4907574022574841No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCT2360.48257811221985925No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCC2270.46417470963520363No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTATACAC2090.4273679044658924No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGT1830.3742025192213316No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTA1650.3373957140520203No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGC1460.2985440863733028No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCC1150.23515458858171112No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC910.1860788483559627No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGCCGTCT910.1860788483559627No Hit
CTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGCCCA840.17176509079011942No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGCCGTCT830.16972026828071324No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTCTTATA760.15540651071486994No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAA730.1492720431866514No Hit
ATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCG710.14518239816783904No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC690.14109275314902667No Hit
CTGCAGGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA680.13904793063962048No Hit
GACTCTGGGGGGAGCAATGCGTGGCCATCATGTCTGAGGAGGATGGAGATGA680.13904793063962048No Hit
ACGGCCGGGGGCGCCTGCCTGATCCTCCAAATCACCACAGGACTATTCCTAG660.1349582856208081No Hit
CTAGGTGGGGTTAGCAGAAACTTACTCTGAGTGTGAATGGAGGATCTGACTG660.1349582856208081No Hit
CTTGCGCGGGATTACAAGTTCAAAATCTTGAGGTAGTTTCTGTGATGGAATA630.12882381809258958No Hit
TCATGCGGGGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCC620.1267789955831834No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATG610.12473417307377721No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTAT600.12268935056437102No Hit
TAAGCAGGGAATGATGGGTTCTTTAGGGCTCTGATTCTTGTGTAAGAACAGT590.12064452805496481No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTC580.11859970554555864No Hit
TCGCCAGGGATACAAGTTTTTGTGTGAATGTGTTTTCATTTCTCATGGGTAT580.11859970554555864No Hit
GCTTATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC570.11655488303615245No Hit
TATGTTGGGGGGCTGCTTACTTCACAGCAGCACAGCTGATGAAAGGGTGTCA560.11451006052674627No Hit
CTTTGCGGGTACTACCACTGACATGACTTTCCAAAAAACACATAATTTGAAT540.1104204155079339No Hit
AAGAAGGGGCTCAGCAAGGGATCCAGTGTACCTGTCACTTAGACTTTGTGCA540.1104204155079339No Hit
TTTTTTGGGGCTGGCATAGGGAAAATGTGTTAATCATTGTGAAAAGCACTTA540.1104204155079339No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCAGCTCGTATGCCGTCT540.1104204155079339No Hit
TGTAGCGGGGCTACTGAATTTGTCTGAAAATCTTTTCATGACAAAAGAACTA540.1104204155079339No Hit
CTTTGCGGGATCTACGAAAGGGGTGAGTTGATTTTCAGAATGAGTTATTGAT540.1104204155079339No Hit
TACTTCCGGGAGATTTTCTGTCTTCAAAGTTTCCGGCTAAACCTCTTCAAGT520.10633077048912154No Hit
ATTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATC510.10428594797971535No Hit
GCTAATGGGAGGGCACAAGATGGTCTGTGATCTTTGCACAGATGAATGGAAG510.10428594797971535No Hit
CGCAAAGGGACCCACACACCCCACTGAGATTTTTGATAGAAGACTTCTTCCC490.10019630296090298No Hit
GCAGGGGGTCAGATCTGAGACCAGCGTCTTCCATCTCTCAGAGCCTTACTCC490.10019630296090298No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)