FastQCFastQC Report
Sat 31 Dec 2022
SRR5849852.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849852.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49058
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGC41378.432875372008644No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCCGTCT36967.533939418647316No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGC7261.4798809572342941No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCA5081.0355089893595337No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGC4260.8683599005259082No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAT2660.5422153369481022No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCC2330.4749480207101798No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCT2250.4586407925312895No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTA2100.4280647396958702No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCTTATAC2090.42602633617350893No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGT1870.38118145868156056No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTATACAC1630.3322597741448897No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCC1230.2507236332504382No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAA1030.20995556280321254No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGC1020.20791715928085122No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGCCGTCT970.19772514166904478No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGCCGTCT930.18957152757959966No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC920.18753312405723838No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATG840.17122589587834808No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC770.15695707122181907No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTCTTATA760.1549186676994578No Hit
GACTCTGGGGGGAGCAATGCGTGGCCATCATGTCTGAGGAGGATGGAGATGA700.14268824656529006No Hit
CTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGCCCA680.1386114395205675No Hit
ATTTATGGGATAAATAAAGCTCCCATTCTTACCCTTTTTTTCTTTTTTCTTT620.12638101838639978No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTC620.12638101838639978No Hit
CTAGGTGGGGTTAGCAGAAACTTACTCTGAGTGTGAATGGAGGATCTGACTG610.12434261486403848No Hit
TTTTTTGGGGCTGGCATAGGGAAAATGTGTTAATCATTGTGAAAAGCACTTA600.1223042113416772No Hit
TCATGCGGGGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCC600.1223042113416772No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTAT590.12026580781931591No Hit
GCTAATGGGAGGGCACAAGATGGTCTGTGATCTTTGCACAGATGAATGGAAG580.11822740429695462No Hit
CTGCAGGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA540.11007379020750949No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCAGCTCGTATGCCGTCT540.11007379020750949No Hit
ACGGCCGGGGGCGCCTGCCTGATCCTCCAAATCACCACAGGACTATTCCTAG530.10803538668514819No Hit
TAAGCAGGGAATGATGGGTTCTTTAGGGCTCTGATTCTTGTGTAAGAACAGT520.1059969831627869No Hit
AAGAAGGGGCTCAGCAAGGGATCCAGTGTACCTGTCACTTAGACTTTGTGCA520.1059969831627869No Hit
TCGTGAGGGAACAAGGCTTGAGGGAGGCACAGCTCACACGTCTGCATGAAAA520.1059969831627869No Hit
CTGCCAGGGAACCATTGGACCTCTTTTTAAAATGCACTGAAGCCTTATAATG500.10192017611806432No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)