FastQCFastQC Report
Sat 31 Dec 2022
SRR5849850.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849850.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134087
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCCGTCT48923.648377545921678No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGC43853.2702648280593944No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGC6820.5086249972033082No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCA4070.30353427252455495No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCTTATAC3910.2916017212705184No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGC3390.25282092969489955No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCT3270.24387151625437217No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTATACAC3100.2311931805469583No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTA2970.2214979826530536No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCC2710.2021075868652442No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAT1900.14169904614168413No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGT1610.12007129699374286No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGC1380.10291825456606532No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)