FastQCFastQC Report
Sat 31 Dec 2022
SRR5849848.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849848.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126832
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGC51454.05654724359783No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCCGTCT48393.815283209284723No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGC8880.7001387662419579No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCA5700.44941339724990537No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGT5610.4423173962406964No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGC4360.3437618266683487No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCTTATAC3560.2806862621420462No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCT3440.2712249274631008No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTATACAC3360.2649173710104705No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGT3250.256244480888104No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTA3000.23653336697363445No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAT2630.2073609183802195No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGC2520.1986880282578529No Hit
CTTAAGGGACAGTTGACTTCTCCCCATTGACTTTGAACAGATTGTTATTATA2130.16793869055128044No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTAT2030.16005424498549262No Hit
TTAATGGGGGCCAATGGAATATTCATGAAAATTGATAATTCAATAAGTTCCC1980.1561120222025987No Hit
GCACTTGGGACACCCAGCCTGTTTTTGTTTTTGTTTGTGAAAGTCACATTTA1930.1521697994197048No Hit
GCTACTGGGATTAAAAAAAGAGCTTTGAGAACTGATGCTGGTATTGATTCAT1840.14507379841049578No Hit
ATCATTGGGCAGGGAAATATAAGGAACTAATTTTGAATAGTTTCATTTTCAA1830.144285353853917No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATG1820.14349690929733822No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCC1790.14113157562760187No Hit
TAATTGGGGGGCTGTCTTGAACAGCTGGAGGGAAGATGCAGGGGTGGGAAGC1780.1403431310710231No Hit
AATTGAGGGACTGCTTTTAAATACCTTCTTCCAAATCAAATTATTCAGTTTT1710.13482401917497164No Hit
TTGTATGGGGGGCCGTGGAACAGAATATATGTTGGACCTCTGAAAAGTTGTA1610.12693957360918381No Hit
CTGCTGGGGAGGTGTTCAACAGATATTTTAGAATGAATGAGAATGAGTGAAG1590.12536268449602625No Hit
GATCAGGGACTTCATGGATAGAGTATCTTCTGTTATTACTGGAAGCTTTTTT1540.12142046171313232No Hit
GATTACGGGGACCTACTGCCAGATACAAGACACTCTGTGCCGAGAAGCATAT1540.12142046171313232No Hit
TGTTCAGGGATCTGTGTATTTATTACCAGTGAGTTTTGTACCTTCAGATAAT1520.11984357259997476No Hit
TAATTTGGGGTATTTTTGCTTTCTTTAGAAATGTAATGTTTGTTATATAGAA1480.11668979437365966No Hit
CGCACGGGGCAGCCAAGGCTACAGAGGATCAAATGCGGCTCCTACGGCTGCA1480.11668979437365966No Hit
TTTGGAGGGGAAGCATCCCAGGGAGATTTAGTGACAATGGTTTCCTCATGAC1480.11668979437365966No Hit
CTACAAGCGGGGTAAGAGGAAGAAAATGGTTAGGACTAGTATTAGTGTAGGA1470.11590134981708086No Hit
GCAATAGGGAATAATCAGATTACTCAAATCAATAAAAGAGAAACTCTCAAAA1470.11590134981708086No Hit
GGCTGGGGTCCATTAATACTTTTGCAAAAAGTGTGCTTGATTTTAAACCTTT1460.1151129052605021No Hit
TCACCAGGGGACTCCAAACTGAGATTCAGCAATTGTTAATTTTGGCCTGGGT1460.1151129052605021No Hit
GACCTGGGAAATTAGAAATATCCAAAGGTGAATTTTTCCACATTCTATCTAG1450.1143244607039233No Hit
TGTTTGGGAGGGAAGTGGCTTAACCTCTCTGAACCTCTTAGTTTTCCTCCTT1440.11353601614734453No Hit
GTACTTGGGATGAAATATTGAATTTTAGGATGATTTGAATATTTATTTATGA1440.11353601614734453No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGCCGTCT1440.11353601614734453No Hit
AGGTTGGGGTGTGGATATTATTTGATTCTGATTTGGAGAAAACAACTGAGAC1430.11274757159076573No Hit
AGTGCAGGGAATCCCATCAAACAGAAGGCAAATGATATGAATAGACAATTCT1430.11274757159076573No Hit
ACTGTAGGGGCAAACTCTGAACTTTTTACAAAGAAAAATGGAAAAACTTTGT1420.11195912703418696No Hit
GGCTGTGGGGATTATACATCTCTCAGTATATTAAGTGAGACTTAACAAGGCC1420.11195912703418696No Hit
AGCAGGGGGATTTCAAGGTGGCGGGGACAGGCTGAAAACGAGACGTTTGTTT1420.11195912703418696No Hit
TGTTGGGGGGAGAAGTTCAGGGGTTCAAGACCAAATTTTCTCTGTGAAGCAA1410.11117068247760817No Hit
TCTCAGGGGGCAGTTTGAAACTATGAATTTTAAAATAGCTATCCAAGGGCTG1380.10880534880787184No Hit
CGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCG1370.10801690425129304No Hit
ACTTGTGGGAAGTAAACACAAGGTTGTTGATAGTTGAAAAAGTAGCGATCAA1370.10801690425129304No Hit
AGCACTGGGGGGAAGCAGTTTCAGATAAATCATGGGCATTGCTGGACTGATG1360.10722845969471427No Hit
CACTCTGGGTCTTGATATGGGATTATTAAAAAGTGTTTGCAAAATGGTCAAG1360.10722845969471427No Hit
TGTCGGGGATGAACAAACAAGGTGAAGGGTTCAGTATAAGTTGGAAATCCTA1350.10644001513813547No Hit
TACGACGGGGAAATTGCAGGAATTACCAGTTACGTCCAACAAGCCAGAGTTC1350.10644001513813547No Hit
GTCAGAGGGGATATTTGTCCCTGACAGATCTGTATTTCTTTGAGAAGAAGAA1340.1056515705815567No Hit
CAACTCAGGGGTTGCCCAGGCTGGAGTGCGGTGGCACGGTCTCGGCTCACTG1330.10486312602497791No Hit
CATTTTGGGATCAATCAGATTTAAATGATACTGTGTTTCATATTGAGGTAAG1320.10407468146839914No Hit
ACATGTGGGATATTTTTGGTGACCTGTTGGTCCCAGTTCAGGGCTAGCTCAC1320.10407468146839914No Hit
TACCATGGGGACCTGTAAGATTATAATTTTAGCTTTTTTCGTTAAGCTTTTC1320.10407468146839914No Hit
CAGACTGGGGACACCTGAAGTCTTACTAAATTCCTGTCCTCAGGCCATCCTT1320.10407468146839914No Hit
GAGACCGGGACATTAGAACCAAGGATGGAATCCTTAGTAAATGGAATCATTT1310.10328623691182034No Hit
CTCTATGGGAAGGCTTGTAACTATGGCTGGATGTTTTGCTCTAGTCTTCTAA1290.10170934779866278No Hit
ACTATTGGGAGAAGCAAAATTTTAGAAAACATCTGACGTGCTAGGATAGGAA1280.10092090324208401No Hit
TACATAGGGAGAATTATTACATTTCTACCGTGGAAAAACATTACTTCAGGTC1280.10092090324208401No Hit
GAGCAAGGGGGAATGTGTTACGATAAATTCATAAGTACGTTTCTATTATTAT1270.10013245868550524No Hit
CAGGCTGGAGTGCAGTGGCATGATCTCAGCTCACTGAAACCTCAGCCTCCTG1270.10013245868550524No Hit
AACCAAGGGAGTAGCTTGAAATCTAGTCTGGAGAAAACTGGATCAAGATTCT1270.10013245868550524No Hit
TCCAGTGGGAGAACCATTTGGAAAAATGAAATTTAGTAGTTCCAAGTTTCAA1270.10013245868550524No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)