FastQCFastQC Report
Sat 31 Dec 2022
SRR5849847.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849847.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124625
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGC50984.090672016048144No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCCGTCT47293.7945837512537612No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGC8540.6852557673019057No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCA5900.473420260782347No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGT5470.4389167502507523No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGC3860.3097291875626881No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCT3660.29368104312938814No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCTTATAC3380.2712136409227683No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTATACAC3110.24954864593781345No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAT2970.2383149448345035No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTA2900.23269809428284857No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGT2800.22467402206619858No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATG1980.158876629889669No Hit
TTAATGGGGGCCAATGGAATATTCATGAAAATTGATAATTCAATAAGTTCCC1980.158876629889669No Hit
CTTAAGGGACAGTTGACTTCTCCCCATTGACTTTGAACAGATTGTTATTATA1980.158876629889669No Hit
GCAATAGGGAATAATCAGATTACTCAAATCAATAAAAGAGAAACTCTCAAAA1950.156469408224674No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTAT1850.14844533600802406No Hit
ATCATTGGGCAGGGAAATATAAGGAACTAATTTTGAATAGTTTCATTTTCAA1810.1452357071213641No Hit
TTGTATGGGGGGCCGTGGAACAGAATATATGTTGGACCTCTGAAAAGTTGTA1770.14202607823470412No Hit
GCTACTGGGATTAAAAAAAGAGCTTTGAGAACTGATGCTGGTATTGATTCAT1740.13961885656970913No Hit
TCTCAGGGGGCAGTTTGAAACTATGAATTTTAAAATAGCTATCCAAGGGCTG1700.13640922768304914No Hit
TAATTGGGGGGCTGTCTTGAACAGCTGGAGGGAAGATGCAGGGGTGGGAAGC1700.13640922768304914No Hit
GATTACGGGGACCTACTGCCAGATACAAGACACTCTGTGCCGAGAAGCATAT1680.13480441323971915No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGC1680.13480441323971915No Hit
CTGCTGGGGAGGTGTTCAACAGATATTTTAGAATGAATGAGAATGAGTGAAG1660.13319959879638918No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCC1650.13239719157472415No Hit
AGTGCAGGGAATCCCATCAAACAGAAGGCAAATGATATGAATAGACAATTCT1640.13159478435305919No Hit
AATTGAGGGACTGCTTTTAAATACCTTCTTCCAAATCAAATTATTCAGTTTT1560.1251755265797392No Hit
TCACCAGGGGACTCCAAACTGAGATTCAGCAATTGTTAATTTTGGCCTGGGT1560.1251755265797392No Hit
AGCAGGGGGATTTCAAGGTGGCGGGGACAGGCTGAAAACGAGACGTTTGTTT1530.12276830491474422No Hit
CGCACGGGGCAGCCAAGGCTACAGAGGATCAAATGCGGCTCCTACGGCTGCA1530.12276830491474422No Hit
TGTTTGGGAGGGAAGTGGCTTAACCTCTCTGAACCTCTTAGTTTTCCTCCTT1510.12116349047141424No Hit
GGCTGTGGGGATTATACATCTCTCAGTATATTAAGTGAGACTTAACAAGGCC1510.12116349047141424No Hit
GCACTTGGGACACCCAGCCTGTTTTTGTTTTTGTTTGTGAAAGTCACATTTA1510.12116349047141424No Hit
TACATAGGGAGAATTATTACATTTCTACCGTGGAAAAACATTACTTCAGGTC1460.11715145436308927No Hit
GTCAGAGGGGATATTTGTCCCTGACAGATCTGTATTTCTTTGAGAAGAAGAA1440.11554663991975928No Hit
CATAGCAGGATTACTTCTGCCAGGAAAGCTAGGAAATATGAAATACCTCATT1410.1131394182547643No Hit
GTACTTGGGATGAAATATTGAATTTTAGGATGATTTGAATATTTATTTATGA1410.1131394182547643No Hit
AGCACTGGGGGGAAGCAGTTTCAGATAAATCATGGGCATTGCTGGACTGATG1400.11233701103309929No Hit
GACCTGGGAAATTAGAAATATCCAAAGGTGAATTTTTCCACATTCTATCTAG1400.11233701103309929No Hit
CATTTTGGGATCAATCAGATTTAAATGATACTGTGTTTCATATTGAGGTAAG1380.11073219658976931No Hit
GCCCAGGGGATAGGAAGGTAAAGTTGCTACCCTTCACTCACAATAAAATGTT1380.11073219658976931No Hit
AGGTTGGGGTGTGGATATTATTTGATTCTGATTTGGAGAAAACAACTGAGAC1370.10992978936810432No Hit
GATCAGGGACTTCATGGATAGAGTATCTTCTGTTATTACTGGAAGCTTTTTT1360.10912738214643933No Hit
TCAACGGGGGTTGTACTAAAAATTTTAGAGACTGCATAGGTCTCCCTTTTCT1350.10832497492477432No Hit
TGTTGGGGGGAGAAGTTCAGGGGTTCAAGACCAAATTTTCTCTGTGAAGCAA1340.10752256770310932No Hit
TAATTTGGGGTATTTTTGCTTTCTTTAGAAATGTAATGTTTGTTATATAGAA1330.10672016048144434No Hit
CGGTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCT1310.10511534603811433No Hit
GGCTGGGGTCCATTAATACTTTTGCAAAAAGTGTGCTTGATTTTAAACCTTT1310.10511534603811433No Hit
ACATGTGGGATATTTTTGGTGACCTGTTGGTCCCAGTTCAGGGCTAGCTCAC1310.10511534603811433No Hit
ATAGTTGGGAATGCCCTTCAGTCCACACTGTGTTACTAGGCCCAAAGCAGTG1310.10511534603811433No Hit
CGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCG1300.10431293881644935No Hit
TTTGGAGGGGAAGCATCCCAGGGAGATTTAGTGACAATGGTTTCCTCATGAC1300.10431293881644935No Hit
CATACTGGGATTGAAAGATCTTACCGAGCGGGGCCTATGGCATGAAGAGATG1270.10190571715145436No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTC1270.10190571715145436No Hit
TCTAGCGGGAGGTCAAGGTACACGGCCCAGCACTTACTCAGATGAGGTGATA1270.10190571715145436No Hit
AGAGGAGGGAAGATTTGTAATGGACTGAGGAATGCTTGTTCTAGCTGCTGAA1260.10110330992978936No Hit
CTTTCAGGGAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCA1260.10110330992978936No Hit
GCGCTAGGGTCACATAAATCTAAATTCTGTGTTCTATATTTGCTTCATAATT1260.10110330992978936No Hit
GTTTTAGGGGCTCAACTGGCATTGCTGTAACCCGTTCCATTTAATTGGTTTT1260.10110330992978936No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)