FastQCFastQC Report
Sat 31 Dec 2022
SRR5849846.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849846.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124171
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGC48003.8656369039469762No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCCGTCT47153.797182917106249No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGC9280.7473564680964154No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCA5460.43971619782396854No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGT5320.42844142352078984No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGC4600.3704568699615852No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTATACAC3430.27623197042787767No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCTTATAC3150.25368242182152034No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTA3040.2448236705833085No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCT2990.2407969654750304No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGT2870.231132873215163No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAT2690.2166367348253618No Hit
ATCATTGGGCAGGGAAATATAAGGAACTAATTTTGAATAGTTTCATTTTCAA2100.1691216145476802No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTAT2040.1642895684177465No Hit
CTTAAGGGACAGTTGACTTCTCCCCATTGACTTTGAACAGATTGTTATTATA2040.1642895684177465No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATG1900.1530147941145678No Hit
TTAATGGGGGCCAATGGAATATTCATGAAAATTGATAATTCAATAAGTTCCC1830.14737740696297846No Hit
GCACTTGGGACACCCAGCCTGTTTTTGTTTTTGTTTGTGAAAGTCACATTTA1720.13851865572476665No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCC1640.1320759275515217No Hit
AGTGCAGGGAATCCCATCAAACAGAAGGCAAATGATATGAATAGACAATTCT1620.13046524550821045No Hit
GCTACTGGGATTAAAAAAAGAGCTTTGAGAACTGATGCTGGTATTGATTCAT1610.12965990448655484No Hit
GACCTGGGAAATTAGAAATATCCAAAGGTGAATTTTTCCACATTCTATCTAG1600.1288545634648992No Hit
AATTGAGGGACTGCTTTTAAATACCTTCTTCCAAATCAAATTATTCAGTTTT1590.1280492224432436No Hit
TTTGGAGGGGAAGCATCCCAGGGAGATTTAGTGACAATGGTTTCCTCATGAC1570.12643854039993235No Hit
GCCCAGGGGATAGGAAGGTAAAGTTGCTACCCTTCACTCACAATAAAATGTT1550.12482785835662112No Hit
GCAATAGGGAATAATCAGATTACTCAAATCAATAAAAGAGAAACTCTCAAAA1540.12402251733496548No Hit
TCTCAGGGGGCAGTTTGAAACTATGAATTTTAAAATAGCTATCCAAGGGCTG1520.12241183529165425No Hit
TCACCAGGGGACTCCAAACTGAGATTCAGCAATTGTTAATTTTGGCCTGGGT1500.12080115324834301No Hit
CTGCTGGGGAGGTGTTCAACAGATATTTTAGAATGAATGAGAATGAGTGAAG1490.11999581222668738No Hit
TAATTGGGGGGCTGTCTTGAACAGCTGGAGGGAAGATGCAGGGGTGGGAAGC1490.11999581222668738No Hit
AGGTTGGGGTGTGGATATTATTTGATTCTGATTTGGAGAAAACAACTGAGAC1480.11919047120503178No Hit
CGCACGGGGCAGCCAAGGCTACAGAGGATCAAATGCGGCTCCTACGGCTGCA1480.11919047120503178No Hit
AGCAGGGGGATTTCAAGGTGGCGGGGACAGGCTGAAAACGAGACGTTTGTTT1470.11838513018337614No Hit
TGTTGGGGGGAGAAGTTCAGGGGTTCAAGACCAAATTTTCTCTGTGAAGCAA1460.11757978916172053No Hit
TTATCAGGGATAGTAAGCAGGCAGTAATAGTTACATGACAATACTGACAATA1460.11757978916172053No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGC1450.11677444814006491No Hit
GTCAGAGGGGATATTTGTCCCTGACAGATCTGTATTTCTTTGAGAAGAAGAA1440.1159691071184093No Hit
TCAACGGGGGTTGTACTAAAAATTTTAGAGACTGCATAGGTCTCCCTTTTCT1430.11516376609675366No Hit
CTTTCAGGGAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCA1400.1127477430317868No Hit
TTGTATGGGGGGCCGTGGAACAGAATATATGTTGGACCTCTGAAAAGTTGTA1370.11033171996681994No Hit
CAGGCTGGAGTGCAGTGGCATGATCTCAGCTCACTGAAACCTCAGCCTCCTG1360.10952637894516433No Hit
TACCATGGGGACCTGTAAGATTATAATTTTAGCTTTTTTCGTTAAGCTTTTC1350.1087210379235087No Hit
GCGCTAGGGTCACATAAATCTAAATTCTGTGTTCTATATTTGCTTCATAATT1330.10711035588019746No Hit
AGGAATGGGATTCTTTGGTTATGTATCTCTGCACATACCTTGCCTAATTTGT1320.10630501485854185No Hit
GATCAGGGACTTCATGGATAGAGTATCTTCTGTTATTACTGGAAGCTTTTTT1320.10630501485854185No Hit
GGCTGGGGTCCATTAATACTTTTGCAAAAAGTGTGCTTGATTTTAAACCTTT1320.10630501485854185No Hit
CATACTGGGATTGAAAGATCTTACCGAGCGGGGCCTATGGCATGAAGAGATG1320.10630501485854185No Hit
CTACAAGCGGGGTAAGAGGAAGAAAATGGTTAGGACTAGTATTAGTGTAGGA1320.10630501485854185No Hit
TAATTTGGGGTATTTTTGCTTTCTTTAGAAATGTAATGTTTGTTATATAGAA1310.10549967383688623No Hit
ACTATTGGGAGAAGCAAAATTTTAGAAAACATCTGACGTGCTAGGATAGGAA1310.10549967383688623No Hit
GGCTGTGGGGATTATACATCTCTCAGTATATTAAGTGAGACTTAACAAGGCC1300.10469433281523062No Hit
GTACTTGGGATGAAATATTGAATTTTAGGATGATTTGAATATTTATTTATGA1290.10388899179357498No Hit
TGTGCCGGGAGAAAAAGGTCAGGCACAGTGCCTACTGAAACATTGAGAGTAA1290.10388899179357498No Hit
ATTAAAGGGAAATCTGGATTCTTTCTAACATCTTTTTTTTCCCCTACAGTCC1290.10388899179357498No Hit
TACATAGGGAGAATTATTACATTTCTACCGTGGAAAAACATTACTTCAGGTC1290.10388899179357498No Hit
TGTTCAGGGATCTGTGTATTTATTACCAGTGAGTTTTGTACCTTCAGATAAT1290.10388899179357498No Hit
TTTAAGCTGGGCTACCTGTAACATGCACATAGAGCCATGGCCGTAGATTTAT1280.10308365077191937No Hit
GTTCTGGGGCAGGGATGAGGAGCTCACGCAGCCCCTCCATGGGACAGGAAGA1280.10308365077191937No Hit
GTTTTAGGGGCTCAACTGGCATTGCTGTAACCCGTTCCATTTAATTGGTTTT1280.10308365077191937No Hit
TGTTTGGGAGGGAAGTGGCTTAACCTCTCTGAACCTCTTAGTTTTCCTCCTT1270.10227830975026375No Hit
AGCACTGGGGGGAAGCAGTTTCAGATAAATCATGGGCATTGCTGGACTGATG1270.10227830975026375No Hit
GCTGGTGGGGAGCCAAGAAAGTAGTTTCTTGAGATGGAATCTACTCTTGGTG1270.10227830975026375No Hit
TGCCCAGGGAAACTAATATACAAAGTAGATGTTAAGGCAGGAAGCATTTTCC1270.10227830975026375No Hit
GATTACGGGGACCTACTGCCAGATACAAGACACTCTGTGCCGAGAAGCATAT1260.10147296872860814No Hit
AGCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCG1250.1006676277069525No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)