FastQCFastQC Report
Sat 31 Dec 2022
SRR5849845.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849845.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97674
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGC60086.15107398079325No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCCGTCT57885.925834920244896No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGC9610.9838851690316768No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCA6170.6316931834469767No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGC4960.5078117001453816No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCTTATAC3590.3675491942584516No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCT3590.3675491942584516No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTATACAC3360.34400147429203265No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAT3350.34297766038044925No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTA3000.30714417347502915No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGCCGTCT2730.2795011978622765No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGT2380.24366771095685646No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCC2290.2344533857526056No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGC2230.228310502283105No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGCCGTCT1660.1699531093228495No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC1400.14333394762168028No Hit
TCCACAGGGAGAATACAAGTAGAGAAGAGCACTGGCTGGCAAGCATCAACAG1300.13309580850584599No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTCTTATA1240.1269529250363454No Hit
TCGCTTGGGGAGATTACAATGTTTATAAATTTAAGGAAATACAGACCATATT1170.11978622765526138No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATG1170.11978622765526138No Hit
CAAACTGGGGCCTGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACG1160.11876241374367796No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCC1130.11569097200892767No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTC1130.11569097200892767No Hit
TTAGCTGGGACTTTTAAAAAGGTTTAGGCATGTTCATTCACTTACTCCTTAG1110.11364334418576079No Hit
CTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGCCCA1100.11261953027417737No Hit
ATTTTAGGGACTCCCAATGATGGGCAGTTCTAGATTAGATGCAGATGGATTT1090.11159571636259392No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC1080.1105719024510105No Hit
TATCATGGGGAGAGAAAAGTGAGATAAAGAAACTAAAGGCTGTGTCACCTGC1060.10852427462784363No Hit
AAGTATGGGAATCTACTGAACAATCAGAAAAGAAAAAAATGTAATGCAAATT1050.10750046071626021No Hit
TTGCCAGGGATTGTGCATTATTTGAACCCTGTCAATATCTTTTTGAGTAATT1040.10647664680467678No Hit
GGCGATGGGAGGGTAATGCAAATTACAACCACAGGGAGATACCACTACACCC1030.10545283289309336No Hit
TATTAAGGGACATTTTCTTGACTTTTGTTCATGGAAACACTACCCAATTTAC1020.10442901898150991No Hit
GTAAATGGGCCGATCTGCCAAGGGTTCTTGTGACAGCTCTTTTTGTGAAGTT1010.10340520506992648No Hit
TGTCTCTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGA990.10135757724675963No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)