FastQCFastQC Report
Sat 31 Dec 2022
SRR5849844.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849844.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95097
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGC58616.16318075228451No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCCGTCT56605.951817617800772No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGC9190.9663816944803727No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCA6180.649862771696268No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGC4540.4774072788836662No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTATACAC3550.37330304846630286No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCTTATAC3510.36909681693428814No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAT3490.36699370116828084No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCT3200.33649852256117435No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTA3060.321776712199123No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCC2330.2450129867398551No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGT2170.22818806061179636No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGC1780.18717730317465325No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGCCGTCT1580.16614614551457985No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGCCGTCT1410.14826966150351747No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTCTTATA1350.14196031420549543No Hit
TTAGCTGGGACTTTTAAAAAGGTTTAGGCATGTTCATTCACTTACTCCTTAG1240.13039317749245508No Hit
GTAAATGGGCCGATCTGCCAAGGGTTCTTGTGACAGCTCTTTTTGTGAAGTT1210.12723850384344404No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC1170.12303227231142938No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTC1170.12303227231142938No Hit
AGATCCGGGATTCCCTCTTGCTTTTTCTTTGTCTCTCACTCTCTCTTTGAAT1150.12092915654542205No Hit
CTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGCCCA1120.11777448289641103No Hit
TATTAAGGGACATTTTCTTGACTTTTGTTCATGGAAACACTACCCAATTTAC1110.11672292501340736No Hit
GGCGATGGGAGGGTAATGCAAATTACAACCACAGGGAGATACCACTACACCC1110.11672292501340736No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC1100.11567136713040369No Hit
TATCATGGGGAGAGAAAAGTGAGATAAAGAAACTAAAGGCTGTGTCACCTGC1070.11251669348139269No Hit
CAACAAGGGGCAAAGATTACTACTATTAACATTATAGTGTATCACTCAGAAA1070.11251669348139269No Hit
TCGCTTGGGGAGATTACAATGTTTATAAATTTAAGGAAATACAGACCATATT1060.11146513559838901No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCC1060.11146513559838901No Hit
GGATATGGGAGTCCCAGCTATTCAGAAGGCTGAGGTGGGAGGATCTCTTGAG1060.11146513559838901No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATG1000.10515578830036701No Hit
CAAACTGGGGCCTGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACG1000.10515578830036701No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAA980.10305267253435965No Hit
TTCACAGGGACTTTCACTGCATTCAATAAGTTTTGGTATGTTTTGTTTTTAT970.10200111465135599No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)