FastQCFastQC Report
Sat 31 Dec 2022
SRR5849843.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849843.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95469
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCCGTCT56885.9579549382522075No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGC55325.794551110831788No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGC9150.9584262954466896No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGC5820.6096219715300255No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCA5630.5897202233185641No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCT3850.40327226639013714No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCAT3320.34775686348448187No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCCTGTCTCTTATAC3300.3456619426201175No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTATACAC3280.3435670217557532No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACTGTCTCTTA2620.2744346332317297No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATGCC2540.2660549497742723No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGT2060.21577684902952793No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCAACTCGTATGCCGTCT1800.18854287779279136No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGC1570.16445128785260138No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCACCTCGTATGCCGTCT1460.15292922309859744No Hit
GGCGATGGGAGGGTAATGCAAATTACAACCACAGGGAGATACCACTACACCC1440.1508343022342331No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCGTATG1360.1424546187767757No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC1240.1298850935905896No Hit
GTAAATGGGCCGATCTGCCAAGGGTTCTTGTGACAGCTCTTTTTGTGAAGTT1240.1298850935905896No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCC1170.1225528705653144No Hit
TCCACAGGGAGAATACAAGTAGAGAAGAGCACTGGCTGGCAAGCATCAACAG1110.11626810797222134No Hit
CAACAAGGGGCAAAGATTACTACTATTAACATTATAGTGTATCACTCAGAAA1090.114173187107857No Hit
TATTAAGGGACATTTTCTTGACTTTTGTTCATGGAAACACTACCCAATTTAC1080.11312572667567483No Hit
AGATCCGGGATTCCCTCTTGCTTTTTCTTTGTCTCTCACTCTCTCTTTGAAT1080.11312572667567483No Hit
TACGCAGGGGCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGC1070.11207826624349264No Hit
TTAGCTGGGACTTTTAAAAAGGTTTAGGCATGTTCATTCACTTACTCCTTAG1060.11103080581131047No Hit
CTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGCCCA1060.11103080581131047No Hit
TGGATGGGGATAAAAAGAAAGAATCCAAGTAACCTATGAATATAGTATTTGA1020.10684096408258177No Hit
TCGCTTGGGGAGATTACAATGTTTATAAATTTAAGGAAATACAGACCATATT1020.10684096408258177No Hit
TAACTGGGGATTTGTATTGGGATATAATTCACATACCATAAACCCCACCATT1020.10684096408258177No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTC1010.1057935036503996No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTC1000.10474604321821743No Hit
ATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCG990.10369858278603526No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATGCATCTCTGTCTCTTATA970.1016036619216709No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)