FastQCFastQC Report
Sat 31 Dec 2022
SRR5849839.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849839.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57655
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGCCGTCT5941.0302662388344463RNA PCR Primer, Index 36 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGC4520.7839736362847975No Hit
GGCATTGGGTAATGCCAGCACTGTGGGTGGCCAAGGTGGGCAGATCACTTGA1440.24976151244471426No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCTTATAC1200.20813459370392853No Hit
GATCTTGGGGGAATTAGAATCTGGAAAAATGAATTATATACAATATGGAAGG1120.19425895412366664No Hit
TGTCATGGGAGGAAAACCACAGCTTTTGGACCCTAAAAGGTCTGGATTGATC1110.1925244991761339No Hit
AGCACAGGATACTAAAAATGTGACCATAAAAAGATGTAAAGAAAAACAAATC1060.1838522244384702No Hit
GTAATGGGGACCAACTTCTGTCCAAAGTCAAATACAGCCCATATCTACCATA1000.17344549475327378No Hit
TGGGAGGGGACATTTCATATTTATAATGTGCTGAAGGTACCATATTTTAAAT980.1699765848582083No Hit
TCGACTGGGACTTAAACTGTTGTGTGTGTGTCATTTTTATTACATTTGCCAT960.16650767496314284No Hit
TATCGCGGGATAGTACCTCCAGTTGTCAGTAACTGCATTCACTTCTCTGTAT910.15783540022547915No Hit
TTTACCGGCAGAACCATTTGGAAAAATGAAATTTAGTAGTTCCAAGTTTCAA910.15783540022547915No Hit
TGAGTTGGGGCAGAGATGGTTTTTTAATGAAATCCCACCATCTATCTGAGTG900.1561009452779464No Hit
GCTCATGGGATAAGTTTCTCTTTAAATAAGCACATCATTCTGTCTTCCTTAA890.15436649033041366No Hit
CTATTAGGGATTTATCATATGACTGAGTGAAAAAATGCAGTGAGTGAAAGTG890.15436649033041366No Hit
GCTCGAGGGACTCTCAGAAGAGAAATGGGGCTTAATTAAGTTGTTTTTGTGG880.15263203538288092No Hit
TCATTAGGGGAAAAAAATACATCGTTCCAAGATCTGAGTCCTCTTTCATAGC880.15263203538288092No Hit
CCAAGGGGCAAACTCTGAACTTTTTACAAAGAAAAATGGAAAAACTTTGTAT860.14916312548781546No Hit
CTTTATTGGGGCTTAGAATGATTTTTTCTTTCTTCCATTTTATCCCTTCTTT850.14742867054028272No Hit
TTCGCTGGGACATTCCTTCTGCAGACCGGCACAAGAGCAAGCTGATTGCAGG850.14742867054028272No Hit
TTCACTGGGACCCCTGAACTTAAAAGTTATTTTAAAAAAGGCCTCCTTTGGT840.14569421559274998No Hit
AACACTGGGGATAAAAAGTGGTGTGTGTGCGTGCATATGTGCCTGTGTACTT840.14569421559274998No Hit
GAGCCTGGGGGGATTTATGTGCATGTCAAATGGCAATTATTATTTTGGAATA830.14395976064521726No Hit
TACAACGGGATAAAAATTAAATTAAGTTAATATCTAAAAGAAATAATGAAGT830.14395976064521726No Hit
TTTTGTGGGGTATGACCTTGTGAAATACATTACTTATTCATTTTATATCAGT830.14395976064521726No Hit
ATTTATGGGGAAACAGAATAGAGAGCTCCCAAGCAAATCTCTACAAATTTAA830.14395976064521726No Hit
CAAGCCGGGCACCTTCTATCAGAATAGCAAAAGAAGCTGCTTTCATAAACTG820.1422253056976845No Hit
CTGACCGGGATCCCACAATTTAGGTTTTTACCATATGTTGGTATGGTCACCA810.14049085075015177No Hit
GTAGAGGGGAGACTATGGGGTTTGTTCCTTTAACCGATACTGTAGGAAGGAC810.14049085075015177No Hit
TTCTGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA800.13875639580261903No Hit
TTCATGCGGGCATCTAAATTAGAATTGTAAGTATGCAGAAGACTTTGCTCAG800.13875639580261903No Hit
TAAATAGGGATGGAGAGGTTAAACAAGGAAAAACATTGCAAAACAATGAAAA800.13875639580261903No Hit
GCACCAGGGAACTTATCAAAACATAGAATTAAGAAAAATAGCTGTATTGAGG800.13875639580261903No Hit
TATCATGGGGTCCGTGGCCAGTGTGTCAAGGCCGGCTGTGACCATGTGGTGG780.13528748590755355No Hit
CTCATGGGAGATAAACTAATCAAATAAATTAGAATAATGTGACAACATTACA780.13528748590755355No Hit
AAAACCGGGGTGAGAAGATAGAGGCAGAACTGCAGGACATCTGCAATGATGT780.13528748590755355No Hit
TGCATGGGATAGGTACCTTGTACCTAATTTTATATTCATAATTTTGTATATT780.13528748590755355No Hit
CTACGAGGGATGCTAATTTACACCAGCACTTCCAATAACAACTAACTATTCT770.13355303096002083No Hit
GCAACAGGGCTGTGTATCTATCTAGAGGAAAATAAGTCATTATACGAAAAAG770.13355303096002083No Hit
CTCGTAGGGTCTGTCTCAAAAAATAAATAAAATAAAAAAGAGAGAAAGCTAG770.13355303096002083No Hit
GACGCAAGGGCACCCTCTGCAATGACCTTGTCAACTCAGCCCAGGGATTTGT770.13355303096002083No Hit
TCTAAATGCGGGACTTCAAAAAGATCTTTGCTTTTAAAAAAAGTGTTTATCA760.13181857601248806No Hit
CACAGGGGAATGAGTAAATACGTGAGTATTTATTTTTATTTTTACTATTATT760.13181857601248806No Hit
TAATAAGGGGAAAACAGACTTTATTGTTAAAAAGGGAATTTGAGGCTTGGTA760.13181857601248806No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCT760.13181857601248806No Hit
GTTTGCGGGATGACACCAAAAGCTCAGGCAACAAAAGAAAAACAAATGAGAC750.13008412106495534No Hit
TTAGCTGGGCGGCCCTGGAGCTGCCCCTCCCCAGCGTGCCCGCCCCTGCCCC750.13008412106495534No Hit
GCTCTAGGGACCTAAATGCAGTGTGGGGTCATTGCCTTGCTTTGCGATGACA750.13008412106495534No Hit
AATGAGGGGAGAATGAGAAAGTTCACATTCCTCACTTCCATCTACCCATGCC750.13008412106495534No Hit
GCCGGCGGGGGGCCAGACCACGGGAAGGACGGTGTTTGTGTCTGAGGGAGCT740.1283496661174226No Hit
CAAACTGGGTAAGAACTATCTCACTTTCATATGTTTGCTTCATAAAAGGATA740.1283496661174226No Hit
GCAAATGGGAGTCATAGGCTGCAAGTCTATAAGATAATTCCATTGGGGAGGG740.1283496661174226No Hit
CACCAAGGGTTGCTGTTGCTGTTTTTCACAGTACCTGTGCATATTTAGGGGG740.1283496661174226No Hit
TATGTAGGGATATTTGTCTAATAAAACAGAGGCAAGATAATTTAATCAAAAG740.1283496661174226No Hit
CATTACGGGAACGCATGTTTCAAAGGCACGTCTTTCCGTTCGTTGGTTTTCT740.1283496661174226No Hit
ATATGGGGGGCAATAACATATTTTAAAGACTTCTGGATAAAAATGCTAATTT730.12661521116988986No Hit
TTTATGGGGGATAATTAGAGCTAATTTGCAAGTACTGATGATAGGCTCATTC730.12661521116988986No Hit
CACAAAGGGGTAACACCTAAAGTTGCCTGAAGAGCCAGTCATGTACCAGAGT720.12488075622235713No Hit
AAACTGGGTCCACTTACTTACATGATCATTTCGAGAGATGTAATGTTGATTG720.12488075622235713No Hit
CGTACAGGGAGAAGGATTAAATGTTCCCAAAGTAATATATGAAAGGACGTGC720.12488075622235713No Hit
GTTTTGGGGTCCTCTCACATGGTGGCTTTAGGAAGATCCTTGGCCAGGAGGG720.12488075622235713No Hit
ATTTCAGGGATACAAATCTCAGTTATTTGGGAGCTAACCTCAAATGCAACTA720.12488075622235713No Hit
ACATCAGGGATCTATGGCTTTGTTCTTTGTCAGTATCTTTTTGTTCTCAAAC710.12314630127482437No Hit
TACTGAGGGATAACCGCCAACCAAACAAGTACCCCTCTTAATGTTTCAAAAT710.12314630127482437No Hit
TTGCGGGATACAAGAAGAGGCCGGGCACGGTGGCACACGCCTGTAATCCCAG710.12314630127482437No Hit
GTTGCGGGGGTTTAAAGTGCTGAAGGAAATTTATCCTAGAATAGCAAATCTG710.12314630127482437No Hit
TCGACCGGGGATTGACCAGCAAATGGGTCAGAGATGATAAGATTAAGCCATA710.12314630127482437No Hit
ATCTCGGGGAGAAAGACCTGAGAAAACGAGCCTGTTTCAGAGGAACATCGTC710.12314630127482437No Hit
CGTACAGGGAGTTCCTACTGGAAAAAGAGTGGAAATTTATTAAAATCAGAAA710.12314630127482437No Hit
TTAGCATGGGATCATTTACAATTTTGATTTCATTTCAACCATTTAACTGCAT710.12314630127482437No Hit
TGCTAGGGGTCTTGCGACGGGACAGGGACCAATTCACAGACCAGCAGAAACT700.12141184632729164No Hit
TATAAGGGGGCTTAAAATTTGTTCCGTAGGACACAAGTTAATTTACATTTTT700.12141184632729164No Hit
TACTAAGGGGAAGTAGTGAGATATATATATGAATACAGATAACAACACATGT690.11967739137975891No Hit
TGTTGCTGGGACCTTGACTCCGTGGTGACTTCATCTTTTTCATGAGTGTCAT690.11967739137975891No Hit
ACCAAAGGGACTTGGGAGGCTGGGGCAGGAGAATCGCTTGAATCCAGGAGGC690.11967739137975891No Hit
AATACAGGGGACGCTCTTCCCATCTGACAACGGGAATCTGCTTGGTGTTCTA690.11967739137975891No Hit
TCACATGGGATTGTGTGCTTTATTTCTATGATTATTACATTGTAATATATAA690.11967739137975891No Hit
TTACACGGGGTAGGAACCGATCTGCTATTTTTAATTTCCTGTGAGGATAGAG680.11794293643222617No Hit
TATTATGGGATCTGCAGATTTCATATGGCAGGAGAGGAAGCAAGGGGTGGGA680.11794293643222617No Hit
CTCGTTGGGGTTTTTTTAAAAGGGTCAGAATGGTTAACACTCAACCCTTTTT680.11794293643222617No Hit
GTGTCAGGGCAATTTAAAAATACTCTTTTAAAACTTAGATCCTTTTATAGTT680.11794293643222617No Hit
GTGTAAGGGAAGATCATCTCTACAACTAAGATGCTGTAGTTCTATCTTTGCA680.11794293643222617No Hit
GTGATGGGGAGGAGATCTCCAGGTGGAAAGAGTACTTTACAGCACTGGAGGT680.11794293643222617No Hit
ATTAGTGGGCTGCCAGAACTGTAATAAATTTGTGTTGTTCTAAACCATTAGG680.11794293643222617No Hit
TAAAGCTGGGGACCCCAGAGTGGCCCAGCTCATCCCCTACTCTATTCTGTTA680.11794293643222617No Hit
CAAATAGGGGTTCCCTCCAGGCGAGCACGAAGACCTTCTCTTAGAGTCCCAC670.11620848148469344No Hit
ACACTAGGGATTCCAGAGCTGATGTGATGGGCTGTGCAGAAGGGGGCTGTAT670.11620848148469344No Hit
TTAGCTGGGAACCCAGCTGCCCTGAACGCACATAAGGCAGCCCCATGAAGAG670.11620848148469344No Hit
TATTGGGGGACCTCCCTGAATATGTATGGCATAGTTTACTATGTTATGCATA670.11620848148469344No Hit
CAACTGGGGTCCGCCTCCCTTCAGATTCCTTTTGGCACATTCTCCTCTTGAG670.11620848148469344No Hit
GATTAAGGGAAGGAAAATTCTGGATAGTAAAGCAATAGACCTTATGAATGCA660.11447402653716071No Hit
TTGAAGGGGCTTTAATATTGTGTTGGCTATTCTGCGTGTTTTGCCTCTCCAT660.11447402653716071No Hit
GGTGCAGGGACCTTTAACTTTTGGAAACTATTAGTCTTCATAAACTACCCCA660.11447402653716071No Hit
ATATATGGGAGTCAGCAGTGGGCTATTGGTGGTTAAGTTTTGGGGGAGTCAA660.11447402653716071No Hit
TACAGAGGGACCAAAAGGTAACTCTTTCTAGAATCTGGCACACCATTTAATA660.11447402653716071No Hit
GTTTACGGGGAGTTGCAACTATGCTGGCAACTGGAGGCAACAGAAAGAAATG660.11447402653716071No Hit
CCGAATGGGAGGAGACGACAGCTCTGTGCCTGTTATTCTAGAACAAGCCATC660.11447402653716071No Hit
TTCTGGGGGAGCCTTTCAGTGAAATAGGTGACTATTAAAAAAGTAATAGTAT660.11447402653716071No Hit
CTCAGCCGGGCCTCTTCACTTGTGTGTGTGTGTGTAGCGGAAAAGGACAAGA650.11273957158962795No Hit
TGAAGTGGGATCATAAGGTTAGTGAGCAAATTCTGAAATATTTGAACAATCA650.11273957158962795No Hit
TTGCAGGGGCCAGGCCTTGTTCATCAAAACAGGTGACAGTGAGTAACCAAAT650.11273957158962795No Hit
TCGCCAGGGAGGATAATTCCCACTCTATATTAGCATTCTGCTAAAATGTAAT650.11273957158962795No Hit
TATTGAGGGTCACACAGATTTTGAAAAGGGATTCATTATGGCTGAAGTAATG650.11273957158962795No Hit
GAAACAGGGAAGAGTGGGATCTTAAAATCAGAATGTTTCCCTGATGCTGCAG650.11273957158962795No Hit
CACCTGGGGACATCCGCTGACAAATGCATATATAGTGCCAATGTCATACTTG650.11273957158962795No Hit
CCACCAGGGATTACAGTTTTTGGTGAAATTGATGAAGATGGATTTTATTATG650.11273957158962795No Hit
TATCCTGGGATAGGAAGCTCTTCTAGGGGGTAATGGAAAAAGGGTTTTGAGG640.11100511664209523No Hit
TATCTTGGGAAGAAATGAGTCAAGATTTGCCTGTGAGAAAGGACTGAGTATT640.11100511664209523No Hit
AGTATGGGGGTTGGCATATTAATGGATCCACACAATCAAATAGATTAGAAAT640.11100511664209523No Hit
GTGAGTGGGGAAAGATATCTGCTTCCTGCCAGGCTGGATAACTGTTGAATGG640.11100511664209523No Hit
CTAACAGGGGACTGTTTCTTTTTAAGTTACATGTATATTTACCTGACCAATT640.11100511664209523No Hit
GAAGATGGGATCAAGAAACTTTCCCCCTATGTTTCTTACAGAAATTTTATGG630.1092706616945625No Hit
TCATGTGGGATTTTCATTTTTGCTCAATTTTAGAACTAATGGGAACTTTAAA630.1092706616945625No Hit
ACAAATGGGGGATGAACCTCAACACTATCGTGCTAAGTGGAAGAGAAAAAAA630.1092706616945625No Hit
TTCGAAGACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG630.1092706616945625No Hit
ACATACGGGTAGCCATAAACTGATTTCGATGGTAAGCCATGAGAAAGCATCA630.1092706616945625No Hit
CTGGATGGGGCATCAAAATGGTTTCCAGGACAACACCTTATGAACCAAAGAG630.1092706616945625No Hit
TAAATAGGGAGGTTCAAGCAATTCTCATGCCTCAGCTTCCCAAGTAGCTGGG630.1092706616945625No Hit
TAGCTAGGGATAAAAAGAAATACTGTCCCTACCAAAAACAAATACTTTTATG630.1092706616945625No Hit
GGCACCGGGTACATATTGGATGTCACCCCAAAAGCCCCTGACCAGGGGGTTT630.1092706616945625No Hit
GCTGATGGGGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAGATAGAGA620.10753620674702974No Hit
TTCTGTGGGATTATTGATGGACTGATAATTCCCAGGTCTCTTCTGCCAGCTC620.10753620674702974No Hit
TATAAGGGAGATGGTTGGGGGAAGAAGATCAAAAGAATGTTTTGTGACCCGT620.10753620674702974No Hit
CTCAACGGGTGGCTGCATTGAATGATATACGGTATCAGAGACTGGTGGTGGA620.10753620674702974No Hit
TCCGCTGGGGATAGGAGAGTCGGCTATGTCAAATAGGGACTCAAGGCACAAA620.10753620674702974No Hit
AGTTTTGGGGATACAAGAGATGCTTATAGATTCCTAGTTTTTATGTGTTTGG620.10753620674702974No Hit
GTTTAGGGGATTTTCACATATTTTGAGCATCAAGATACTGGTCATTTTAAAA620.10753620674702974No Hit
AATCTGGGGAAGACCACCTAGAGGCTTTTAGCCTTGTCAGCCTGCCCTCTTC620.10753620674702974No Hit
CATTAGGGAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGATT610.10580175179949701No Hit
GAGCTGGGGGTGTGGTTCCAACATTGTATGTGACAAAATAATTTGCCAAGTT610.10580175179949701No Hit
GCTAAGGGGGAAGTCTGTGTCTGTGCACTTCCCACAGACTGGAGTTTTTGGT610.10580175179949701No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACA610.10580175179949701No Hit
CTACATCCGGGGGGATGAAAGGTGCCCTAAGCAGGAGAAACTGAACAAAAGG610.10580175179949701No Hit
GTACTTGGGTCCTATCCAAGAAAGGAGGTGCTGGCCACCAGCAGGTGAAGGG610.10580175179949701No Hit
ATATCGGGGAAGTTAAATAATTGACTGCAATGAAAAATGCTAGGGAGAGTTT610.10580175179949701No Hit
ACAGTAGGGCAAGAGATCCTCCTGTCTCAGTCTCTCAAGTAGCTGAGACTAC610.10580175179949701No Hit
GTTACAGGGAAGAAAAGAAACACTGGTAGTAGAAAGCTGAGTCCTAAAATAT600.10406729685196427No Hit
TTTAAAGGGTTTTCTAAGTGGTTTTTCCCAATACTCTGAAACTAGGATTTAT600.10406729685196427No Hit
ATCACAGGGTATTTATGAAAATTATTCTTTCTAGTTTGTTTTTAAGTGCAAA600.10406729685196427No Hit
GATCATGGGCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAA600.10406729685196427No Hit
TGTCACCGGGTCTTTAGACGGGATCTACAGAGGACTGGTTCCAGGACTCCCA600.10406729685196427No Hit
GAGCGGGGGGGCATATCAGATATGCAAGTCTGTGAATTTTTTGCACAGTAAT600.10406729685196427No Hit
TTATAGGGGACTCCCCTTTGACCTATAAATTTCACTTACAGGAATATATTCA600.10406729685196427No Hit
TCATCCTGGGATGAATTAAAGAATCCAGATAGGTAGCACCTTATCCTTATTC600.10406729685196427No Hit
CGCGGGGGGATTAGAGGTTTGGGCATATAACCAGATTAAAGTGAAGGAACTT600.10406729685196427No Hit
CAGTTGGGGACTCAGGAGGCTGACATGGGAGGATCACTCAAGCCCCGGGAGT600.10406729685196427No Hit
GTGTTGGGGATAAGCAGTTGCTTCAAAATGTGAATTAACTTGTTGAAACTGT600.10406729685196427No Hit
GGTCATGGGAATTTCAGGGGAAAAAATGCCTGATTTGCTATTTTTGACATTT600.10406729685196427No Hit
ACCAAAGGGAGATCTACCATTTGATCCAGCAATCCCACTACTGGATCTACCA590.10233284190443154No Hit
CAAAGAGGGGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAAGCCTAGT590.10233284190443154No Hit
TTTATAGGGTCTGCCTTGTCTGTGTGTGAAGTTCATACCCGGGGTAGATATG590.10233284190443154No Hit
TTGAGTGGGGTATTCATTATAGATGTAGCCCTTATTTAAAAAAGTGAATTCC590.10233284190443154No Hit
TCGCCGGGGATCAGAGATTTTGACTCAGTTTGTTTTGGGAAGGATCTGAATA590.10233284190443154No Hit
AGTCTCGGGACTTGTTACTGATGAAGCAGCAGATGTCACTGAACTATTGTGG580.1005983869568988No Hit
GGTACTGGGATCTGGGTCTGGATCAAAAACGCAGAAAATATGTGGTTGGAGA580.1005983869568988No Hit
TCTTCAGGGACAGCATGAAAAAGCATATTTGTGTTTGAAAATATACAAGAAT580.1005983869568988No Hit
CGTACTGGGAAAGAGAGGGGTTAAACATGCCCTCTAAAAGTAGGTGGTTTTG580.1005983869568988No Hit
GAATCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA580.1005983869568988No Hit
GATGTTGGGAGACTCATGAGGGATCCCAATACCAGTTCCTATGTTGTCAAAA580.1005983869568988No Hit
TATCTGGGGGAGCCAAAAGTCCATGAACCAGTTCGAATTGCCTATGACAGGC580.1005983869568988No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)