FastQCFastQC Report
Sat 31 Dec 2022
SRR5849838.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849838.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56716
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGCCGTCT5310.9362437407433529RNA PCR Primer, Index 36 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGC4570.8057690951407009No Hit
GGCATTGGGTAATGCCAGCACTGTGGGTGGCCAAGGTGGGCAGATCACTTGA1390.24508075322660275No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCTTATAC1260.22215953170181255No Hit
TGTCATGGGAGGAAAACCACAGCTTTTGGACCCTAAAAGGTCTGGATTGATC1160.20452782283658932No Hit
GACGCAAGGGCACCCTCTGCAATGACCTTGTCAACTCAGCCCAGGGATTTGT1080.19042245574441075No Hit
AGCACAGGATACTAAAAATGTGACCATAAAAAGATGTAAAGAAAAACAAATC1070.18865928485788844No Hit
GATCTTGGGGGAATTAGAATCTGGAAAAATGAATTATATACAATATGGAAGG1060.1868961139713661No Hit
TTCGCTGGGACATTCCTTCTGCAGACCGGCACAAGAGCAAGCTGATTGCAGG1030.18160660131179915No Hit
AAAACCGGGGTGAGAAGATAGAGGCAGAACTGCAGGACATCTGCAATGATGT980.17279074687918752No Hit
TTCATGCGGGCATCTAAATTAGAATTGTAAGTATGCAGAAGACTTTGCTCAG970.17102757599266522No Hit
CTTTATTGGGGCTTAGAATGATTTTTTCTTTCTTCCATTTTATCCCTTCTTT960.16926440510614288No Hit
TGAGTTGGGGCAGAGATGGTTTTTTAATGAAATCCCACCATCTATCTGAGTG960.16926440510614288No Hit
GAGCTGGGGGTGTGGTTCCAACATTGTATGTGACAAAATAATTTGCCAAGTT950.16750123421962057No Hit
TATCATGGGGTCCGTGGCCAGTGTGTCAAGGCCGGCTGTGACCATGTGGTGG920.16221172156005362No Hit
TGAAGTGGGATCATAAGGTTAGTGAGCAAATTCTGAAATATTTGAACAATCA920.16221172156005362No Hit
GCTCGAGGGACTCTCAGAAGAGAAATGGGGCTTAATTAAGTTGTTTTTGTGG910.16044855067353128No Hit
TCGACCGGGGATTGACCAGCAAATGGGTCAGAGATGATAAGATTAAGCCATA900.15868537978700895No Hit
TATCGCGGGATAGTACCTCCAGTTGTCAGTAACTGCATTCACTTCTCTGTAT900.15868537978700895No Hit
ATCTCGGGGAGAAAGACCTGAGAAAACGAGCCTGTTTCAGAGGAACATCGTC890.15692220890048664No Hit
AATGAGGGGAGAATGAGAAAGTTCACATTCCTCACTTCCATCTACCCATGCC880.1551590380139643No Hit
ATTTATGGGGAAACAGAATAGAGAGCTCCCAAGCAAATCTCTACAAATTTAA870.153395867127442No Hit
TTTTGTGGGGTATGACCTTGTGAAATACATTACTTATTCATTTTATATCAGT860.15163269624091968No Hit
CTGACCGGGATCCCACAATTTAGGTTTTTACCATATGTTGGTATGGTCACCA860.15163269624091968No Hit
GTGAGTGGGGAAAGATATCTGCTTCCTGCCAGGCTGGATAACTGTTGAATGG860.15163269624091968No Hit
GATCATGGGCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAA850.14986952535439735No Hit
GAGCCTGGGGGGATTTATGTGCATGTCAAATGGCAATTATTATTTTGGAATA850.14986952535439735No Hit
TCGACTGGGACTTAAACTGTTGTGTGTGTGTCATTTTTATTACATTTGCCAT840.14810635446787504No Hit
TACAACGGGATAAAAATTAAATTAAGTTAATATCTAAAAGAAATAATGAAGT840.14810635446787504No Hit
CACCTGGGGACATCCGCTGACAAATGCATATATAGTGCCAATGTCATACTTG840.14810635446787504No Hit
TAAATAGGGATGGAGAGGTTAAACAAGGAAAAACATTGCAAAACAATGAAAA830.1463431835813527No Hit
TACTGAGGGATAACCGCCAACCAAACAAGTACCCCTCTTAATGTTTCAAAAT820.14458001269483037No Hit
CTCGTTGGGGTTTTTTTAAAAGGGTCAGAATGGTTAACACTCAACCCTTTTT820.14458001269483037No Hit
TTAGCTGGGCGGCCCTGGAGCTGCCCCTCCCCAGCGTGCCCGCCCCTGCCCC820.14458001269483037No Hit
AATACAGGGGACGCTCTTCCCATCTGACAACGGGAATCTGCTTGGTGTTCTA820.14458001269483037No Hit
CCGAATGGGAGGAGACGACAGCTCTGTGCCTGTTATTCTAGAACAAGCCATC820.14458001269483037No Hit
CTACGAGGGATGCTAATTTACACCAGCACTTCCAATAACAACTAACTATTCT800.14105367092178575No Hit
AACACTGGGGATAAAAAGTGGTGTGTGTGCGTGCATATGTGCCTGTGTACTT800.14105367092178575No Hit
AAACATGGGGATCTTGAACAGGGGCTGAAAATACAGAGAGAAAGCAAAATCA800.14105367092178575No Hit
CGTACTGGGAAAGAGAGGGGTTAAACATGCCCTCTAAAAGTAGGTGGTTTTG790.13929050003526341No Hit
ACACTAGGGATTCCAGAGCTGATGTGATGGGCTGTGCAGAAGGGGGCTGTAT780.1375273291487411No Hit
GTAATGGGGACCAACTTCTGTCCAAAGTCAAATACAGCCCATATCTACCATA780.1375273291487411No Hit
GCACCAGGGAACTTATCAAAACATAGAATTAAGAAAAATAGCTGTATTGAGG780.1375273291487411No Hit
TATCCTGGGATAGGAAGCTCTTCTAGGGGGTAATGGAAAAAGGGTTTTGAGG770.13576415826221877No Hit
CTCAACGGGTGGCTGCATTGAATGATATACGGTATCAGAGACTGGTGGTGGA770.13576415826221877No Hit
GTGTCAGGGCAATTTAAAAATACTCTTTTAAAACTTAGATCCTTTTATAGTT770.13576415826221877No Hit
TCATTAGGGGAAAAAAATACATCGTTCCAAGATCTGAGTCCTCTTTCATAGC770.13576415826221877No Hit
TCGCCAGGGAGGATAATTCCCACTCTATATTAGCATTCTGCTAAAATGTAAT770.13576415826221877No Hit
CGCGGGGGGATTAGAGGTTTGGGCATATAACCAGATTAAAGTGAAGGAACTT770.13576415826221877No Hit
GTAGAGGGGAGACTATGGGGTTTGTTCCTTTAACCGATACTGTAGGAAGGAC770.13576415826221877No Hit
TTTAGGGGGAATAAGGGAAAGATGATCTTACATAATTAACTTCAGTAATTTG760.13400098737569643No Hit
TATTGGGGGACCTCCCTGAATATGTATGGCATAGTTTACTATGTTATGCATA760.13400098737569643No Hit
CTCATGGGAGATAAACTAATCAAATAAATTAGAATAATGTGACAACATTACA750.13223781648917413No Hit
TTTATAGGGTCTGCCTTGTCTGTGTGTGAAGTTCATACCCGGGGTAGATATG750.13223781648917413No Hit
CAAGCCGGGCACCTTCTATCAGAATAGCAAAAGAAGCTGCTTTCATAAACTG750.13223781648917413No Hit
ACCAAAGGGAGATCTACCATTTGATCCAGCAATCCCACTACTGGATCTACCA740.13047464560265182No Hit
CACCAAGGGTTGCTGTTGCTGTTTTTCACAGTACCTGTGCATATTTAGGGGG740.13047464560265182No Hit
GTAAAGGCAGGGCTATAGGATATGAACTTCACAATTCATTTGGGTACATTCT740.13047464560265182No Hit
TTCTGTGGGATTATTGATGGACTGATAATTCCCAGGTCTCTTCTGCCAGCTC730.12871147471612948No Hit
GTGTAAGGGAAGATCATCTCTACAACTAAGATGCTGTAGTTCTATCTTTGCA730.12871147471612948No Hit
CTACATCCGGGGGGATGAAAGGTGCCCTAAGCAGGAGAAACTGAACAAAAGG730.12871147471612948No Hit
CTCGTAGGGTCTGTCTCAAAAAATAAATAAAATAAAAAAGAGAGAAAGCTAG720.12694830382960717No Hit
TATAAGGGAGATGGTTGGGGGAAGAAGATCAAAAGAATGTTTTGTGACCCGT710.12518513294308484No Hit
GCTCATGGGATAAGTTTCTCTTTAAATAAGCACATCATTCTGTCTTCCTTAA710.12518513294308484No Hit
GTTTGCGGGATGACACCAAAAGCTCAGGCAACAAAAGAAAAACAAATGAGAC710.12518513294308484No Hit
CTCATTACGGGTGGTGATAAGAGCAGTAAAGAAGAAAGTGCTGAAAGAACGA710.12518513294308484No Hit
AGGCATGGGATTCTAACTGGTGTGAGATGGTATCTCATTGTGGTTTTGATTT710.12518513294308484No Hit
GCTAAGGGGGAAGTCTGTGTCTGTGCACTTCCCACAGACTGGAGTTTTTGGT700.12342196205656253No Hit
CCAAGGGGCAAACTCTGAACTTTTTACAAAGAAAAATGGAAAAACTTTGTAT700.12342196205656253No Hit
CGCTATGGGGACCATTGTGAAATCCTTTCTCAGTGTAACTGCATTTCTAATA700.12342196205656253No Hit
GTTGCGGGGGTTTAAAGTGCTGAAGGAAATTTATCCTAGAATAGCAAATCTG700.12342196205656253No Hit
GGTGCAGGGACCTTTAACTTTTGGAAACTATTAGTCTTCATAAACTACCCCA700.12342196205656253No Hit
CGTACAGGGAGAAGGATTAAATGTTCCCAAAGTAATATATGAAAGGACGTGC700.12342196205656253No Hit
GCTCTAGGGACCTAAATGCAGTGTGGGGTCATTGCCTTGCTTTGCGATGACA700.12342196205656253No Hit
TAGCTAGGGATAAAAAGAAATACTGTCCCTACCAAAAACAAATACTTTTATG700.12342196205656253No Hit
TCTAAATGCGGGACTTCAAAAAGATCTTTGCTTTTAAAAAAAGTGTTTATCA690.12165879117004019No Hit
TCATGTGGGATTTTCATTTTTGCTCAATTTTAGAACTAATGGGAACTTTAAA690.12165879117004019No Hit
ACATACGGGTAGCCATAAACTGATTTCGATGGTAAGCCATGAGAAAGCATCA690.12165879117004019No Hit
TTTACCGGCAGAACCATTTGGAAAAATGAAATTTAGTAGTTCCAAGTTTCAA690.12165879117004019No Hit
CTAACAGGGGACTGTTTCTTTTTAAGTTACATGTATATTTACCTGACCAATT690.12165879117004019No Hit
TTGGTCGGGGATTTACAGGTTTAATTGTAACCATAGGATGCTATTGATCTGT690.12165879117004019No Hit
TGTTGCTGGGACCTTGACTCCGTGGTGACTTCATCTTTTTCATGAGTGTCAT680.11989562028351787No Hit
TTGTTAGGGGGTTTACTTTCTGCTGCTAAAAACCACGTCAGTGACAACTGCT680.11989562028351787No Hit
TAAATAGGGAGGTTCAAGCAATTCTCATGCCTCAGCTTCCCAAGTAGCTGGG680.11989562028351787No Hit
TGGGAGGGGACATTTCATATTTATAATGTGCTGAAGGTACCATATTTTAAAT680.11989562028351787No Hit
GTGTTGGGGATAAGCAGTTGCTTCAAAATGTGAATTAACTTGTTGAAACTGT680.11989562028351787No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCT680.11989562028351787No Hit
ACATCAGGGATCTATGGCTTTGTTCTTTGTCAGTATCTTTTTGTTCTCAAAC670.11813244939699555No Hit
CTAGAAGGGGTAGGGTTTTCATAGTGGACAAAGAACTTGTGGTCTTTTAAAA670.11813244939699555No Hit
CGCGTTGGGATTCAAAGTGATACAGTAATAAACATCAAATATGTTTGAGAAG670.11813244939699555No Hit
CTATTAGGGATTTATCATATGACTGAGTGAAAAAATGCAGTGAGTGAAAGTG670.11813244939699555No Hit
CTAGTTACGGGATGTACAGTTGGCCCTATGTATCCATGGGTACACATCTGTG670.11813244939699555No Hit
TCTAAGGGAGTAAGGAGACAGTGAGAATGAAGAATGAAAGAAACCCATGGAT670.11813244939699555No Hit
CATTACGGGAACGCATGTTTCAAAGGCACGTCTTTCCGTTCGTTGGTTTTCT670.11813244939699555No Hit
CACAAAGGGGTAACACCTAAAGTTGCCTGAAGAGCCAGTCATGTACCAGAGT660.11636927851047324No Hit
CAAATAGGGGTTCCCTCCAGGCGAGCACGAAGACCTTCTCTTAGAGTCCCAC660.11636927851047324No Hit
AAAGTTGGGGAGTTTTAATTTTAAGGTATACTTGTAGGTGGATGATGAAGAC660.11636927851047324No Hit
ACAAATGGGGGATGAACCTCAACACTATCGTGCTAAGTGGAAGAGAAAAAAA660.11636927851047324No Hit
GCTGATGGGGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAGATAGAGA650.11460610762395092No Hit
GAGCGGGGGGGCATATCAGATATGCAAGTCTGTGAATTTTTTGCACAGTAAT650.11460610762395092No Hit
GTGATGGGGAGGAGATCTCCAGGTGGAAAGAGTACTTTACAGCACTGGAGGT650.11460610762395092No Hit
TATACGGGAGCCACTGTGCCTGGCTGTGTTTTTAATAAATAGCAAAAGAAGC650.11460610762395092No Hit
GCTAACGGGATTCTGACTTTGCTTTACAGTCTAGCACTTAGTTTCTTAGCTT640.1128429367374286No Hit
GCAAATGGGAGTCATAGGCTGCAAGTCTATAAGATAATTCCATTGGGGAGGG640.1128429367374286No Hit
AGTATGGGGGTTGGCATATTAATGGATCCACACAATCAAATAGATTAGAAAT640.1128429367374286No Hit
TTAGCATGGGATCATTTACAATTTTGATTTCATTTCAACCATTTAACTGCAT640.1128429367374286No Hit
CACTCAGGGGTGTAATCATAAAATAGCTAATTTTGTCGTGCTTAATGCCAAG640.1128429367374286No Hit
AACTGAGGGACAGAAGGAAGGGAGAGAAAGAACTCATTTATGGAACACCTAC630.11107976585090627No Hit
CGTCAGGGGTGACTTGTTCACTTTGTGTTTGTCCAGCTTCCATGGCATGTGG630.11107976585090627No Hit
TTGCGGGATACAAGAAGAGGCCGGGCACGGTGGCACACGCCTGTAATCCCAG630.11107976585090627No Hit
TGTCGGGGGGCATCTTCTGAACCAGAGGCATTTCTGATTAGCCCTTCCCTAC630.11107976585090627No Hit
TTCTGGGGGAGCCTTTCAGTGAAATAGGTGACTATTAAAAAAGTAATAGTAT630.11107976585090627No Hit
CTCCATGGGTAGCCGCGGGCAATTTCATAACTAGAGTATAAAGTAATTGTAT630.11107976585090627No Hit
GTTACAGGGAAGAAAAGAAACACTGGTAGTAGAAAGCTGAGTCCTAAAATAT620.10931659496438396No Hit
CACAATGGGCGATCTGCGATAGGGACCTCACAAATATATAAAGCATTTAAAT620.10931659496438396No Hit
CAAACTGGGTAAGAACTATCTCACTTTCATATGTTTGCTTCATAAAAGGATA620.10931659496438396No Hit
TTGCAGGGGCCAGGCCTTGTTCATCAAAACAGGTGACAGTGAGTAACCAAAT620.10931659496438396No Hit
GAAACAGGGAAGAGTGGGATCTTAAAATCAGAATGTTTCCCTGATGCTGCAG620.10931659496438396No Hit
GATGTTGGGAGACTCATGAGGGATCCCAATACCAGTTCCTATGTTGTCAAAA620.10931659496438396No Hit
TATGTAGGGATATTTGTCTAATAAAACAGAGGCAAGATAATTTAATCAAAAG620.10931659496438396No Hit
TGGCTTGGGGATCTAGAGATTCAATGCAATCCCTATCAAAATGCCAACTGCC620.10931659496438396No Hit
TTTAAAGGGTTTTCTAAGTGGTTTTTCCCAATACTCTGAAACTAGGATTTAT610.10755342407786161No Hit
GCCGGCGGGGGGCCAGACCACGGGAAGGACGGTGTTTGTGTCTGAGGGAGCT610.10755342407786161No Hit
GTCAGCGGGGACCTTGAGCACTTCGTTAAGGTGATGTCTGTCAGGTGTCTTC610.10755342407786161No Hit
AGATGTGGGAGACACTAAAAAACATTATTTCACCTCCGTGGGATTTCAGGGA610.10755342407786161No Hit
ACGAATGGGATTTTAAGGCAGGTATGATAGCAACATATTGTTCCAGCTTTGG610.10755342407786161No Hit
AGCGATGGGAGCTGCAATTCTCATTGCAAGATCATCGCCTCCCTGGTTTATA610.10755342407786161No Hit
CATCGCGGAGTGTTCAGATTTAATTGAAGTCTCATTTTAGGGTCATGCTGCA610.10755342407786161No Hit
TCGCCAGGGAGCACCCATTTTTGAGAAAGCTGGTTCTACATGGGGGGATAGC610.10755342407786161No Hit
GTTTACGGGGAGTTGCAACTATGCTGGCAACTGGAGGCAACAGAAAGAAATG610.10755342407786161No Hit
TTTGGTAGGGAGTCTCAAAATACCTTCTCACAAAATGCTTATCAACTAAAGT610.10755342407786161No Hit
CAGTGGGGATGAATTTGATGCAATTCTAATCAAAATCTAATTAACATTTTTG610.10755342407786161No Hit
TATGCCGGGATGTGTATTTGTGTTCTATTTAGGTTCCAGATGTGGAATCTCG600.1057902531913393No Hit
AAATAAGGGAATGTGGTTATTAAAAGAAGGCTGATTTTTACCATACACCATT600.1057902531913393No Hit
CAAAGAGGGGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAAGCCTAGT600.1057902531913393No Hit
ACTTAGGGGTTGGGTGGTGGTGGAGTGGATGTGGGCATTTTGAGGATCCAGC600.1057902531913393No Hit
TCACATGGGATTGTGTGCTTTATTTCTATGATTATTACATTGTAATATATAA600.1057902531913393No Hit
GTTTTGGGGTCCTCTCACATGGTGGCTTTAGGAAGATCCTTGGCCAGGAGGG600.1057902531913393No Hit
TAAAGCTGGGGACCCCAGAGTGGCCCAGCTCATCCCCTACTCTATTCTGTTA600.1057902531913393No Hit
TATAAGGGGGCTTAAAATTTGTTCCGTAGGACACAAGTTAATTTACATTTTT600.1057902531913393No Hit
CCACCAGGGATTACAGTTTTTGGTGAAATTGATGAAGATGGATTTTATTATG600.1057902531913393No Hit
ATACTTGGGCACGAAGATATCATATGAGGATGACAATGATCTTTAACAGATT590.10402708230481698No Hit
TATCTTGGGAAGAAATGAGTCAAGATTTGCCTGTGAGAAAGGACTGAGTATT590.10402708230481698No Hit
TTCGAAGACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG590.10402708230481698No Hit
CGTACAGGGAGTTCCTACTGGAAAAAGAGTGGAAATTTATTAAAATCAGAAA590.10402708230481698No Hit
ACTTCAGGGAGAAATTACCAGTGTAACTAAACATAAATATTCCAGTTTAGAG590.10402708230481698No Hit
ATATCGGGGAAGTTAAATAATTGACTGCAATGAAAAATGCTAGGGAGAGTTT590.10402708230481698No Hit
TCTTCAGGGACAGCATGAAAAAGCATATTTGTGTTTGAAAATATACAAGAAT580.10226391141829466No Hit
GATTAAGGGAAGGAAAATTCTGGATAGTAAAGCAATAGACCTTATGAATGCA580.10226391141829466No Hit
ACCAAAGGGACTTGGGAGGCTGGGGCAGGAGAATCGCTTGAATCCAGGAGGC580.10226391141829466No Hit
TCCGCTGGGGATAGGAGAGTCGGCTATGTCAAATAGGGACTCAAGGCACAAA580.10226391141829466No Hit
GGGACAGGGAGGCTGTTCCTTGTACTCTTAGGATATTTGAGTCACTTGTGTC580.10226391141829466No Hit
TGTGCTGGGAGTAGTGTAAAGTCCCTGGCAGCCTTGGAGCAGAGCGATAACA580.10226391141829466No Hit
TTGAGTGGGGTATTCATTATAGATGTAGCCCTTATTTAAAAAAGTGAATTCC580.10226391141829466No Hit
GTTTAGGGGATTTTCACATATTTTGAGCATCAAGATACTGGTCATTTTAAAA580.10226391141829466No Hit
TGCATGGGATAGGTACCTTGTACCTAATTTTATATTCATAATTTTGTATATT580.10226391141829466No Hit
TACTAAGGGGAAGTAGTGAGATATATATATGAATACAGATAACAACACATGT570.10050074053177234No Hit
TGCTAGGGGTCTTGCGACGGGACAGGGACCAATTCACAGACCAGCAGAAACT570.10050074053177234No Hit
TAATAAGGGGAAAACAGACTTTATTGTTAAAAAGGGAATTTGAGGCTTGGTA570.10050074053177234No Hit
ACGCTTTGGGACATATGTTATGCCTTTATTTAAAAGAATATGAAGAAGTCAT570.10050074053177234No Hit
TATTGAGGGTCACACAGATTTTGAAAAGGGATTCATTATGGCTGAAGTAATG570.10050074053177234No Hit
GTACTTGGGTCCTATCCAAGAAAGGAGGTGCTGGCCACCAGCAGGTGAAGGG570.10050074053177234No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)