FastQCFastQC Report
Sat 31 Dec 2022
SRR5849837.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849837.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58372
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGCCGTCT5770.9884876310559858RNA PCR Primer, Index 36 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGC4540.7777701637771534No Hit
GGCATTGGGTAATGCCAGCACTGTGGGTGGCCAAGGTGGGCAGATCACTTGA1260.21585691770026724No Hit
AGCACAGGATACTAAAAATGTGACCATAAAAAGATGTAAAGAAAAACAAATC1220.209004317138354No Hit
GATCTTGGGGGAATTAGAATCTGGAAAAATGAATTATATACAATATGGAAGG1200.20557801685739738No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCTTATAC1140.1952991160145275No Hit
TGTCATGGGAGGAAAACCACAGCTTTTGGACCCTAAAAGGTCTGGATTGATC1140.1952991160145275No Hit
TATCATGGGGTCCGTGGCCAGTGTGTCAAGGCCGGCTGTGACCATGTGGTGG1060.181593914890701No Hit
ATCTCGGGGAGAAAGACCTGAGAAAACGAGCCTGTTTCAGAGGAACATCGTC1050.17988076475022272No Hit
TCATTAGGGGAAAAAAATACATCGTTCCAAGATCTGAGTCCTCTTTCATAGC1040.1781676146097444No Hit
CCAAGGGGCAAACTCTGAACTTTTTACAAAGAAAAATGGAAAAACTTTGTAT1020.17474131432878778No Hit
GTTTGCGGGATGACACCAAAAGCTCAGGCAACAAAAGAAAAACAAATGAGAC1010.17302816418830946No Hit
GTGTAAGGGAAGATCATCTCTACAACTAAGATGCTGTAGTTCTATCTTTGCA950.16274926334543957No Hit
TACTGAGGGATAACCGCCAACCAAACAAGTACCCCTCTTAATGTTTCAAAAT930.159322963064483No Hit
GAGCCTGGGGGGATTTATGTGCATGTCAAATGGCAATTATTATTTTGGAATA920.15760981292400467No Hit
AATGAGGGGAGAATGAGAAAGTTCACATTCCTCACTTCCATCTACCCATGCC900.15418351264304803No Hit
CACCAAGGGTTGCTGTTGCTGTTTTTCACAGTACCTGTGCATATTTAGGGGG890.15247036250256973No Hit
CGTACAGGGAGAAGGATTAAATGTTCCCAAAGTAATATATGAAAGGACGTGC890.15247036250256973No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCT890.15247036250256973No Hit
TGAAGTGGGATCATAAGGTTAGTGAGCAAATTCTGAAATATTTGAACAATCA880.15075721236209141No Hit
CGTACAGGGAGTTCCTACTGGAAAAAGAGTGGAAATTTATTAAAATCAGAAA880.15075721236209141No Hit
CTATTAGGGATTTATCATATGACTGAGTGAAAAAATGCAGTGAGTGAAAGTG880.15075721236209141No Hit
TCTAAATGCGGGACTTCAAAAAGATCTTTGCTTTTAAAAAAAGTGTTTATCA870.1490440622216131No Hit
GCTCGAGGGACTCTCAGAAGAGAAATGGGGCTTAATTAAGTTGTTTTTGTGG860.14733091208113477No Hit
TATCGCGGGATAGTACCTCCAGTTGTCAGTAACTGCATTCACTTCTCTGTAT860.14733091208113477No Hit
GTGTTGGGGATAAGCAGTTGCTTCAAAATGTGAATTAACTTGTTGAAACTGT860.14733091208113477No Hit
TCGACTGGGACTTAAACTGTTGTGTGTGTGTCATTTTTATTACATTTGCCAT850.14561776194065648No Hit
CTTTATTGGGGCTTAGAATGATTTTTTCTTTCTTCCATTTTATCCCTTCTTT850.14561776194065648No Hit
CTCCATGGGTAGCCGCGGGCAATTTCATAACTAGAGTATAAAGTAATTGTAT840.1439046118001782No Hit
TCGACCGGGGATTGACCAGCAAATGGGTCAGAGATGATAAGATTAAGCCATA830.14219146165969984No Hit
TTCATGCGGGCATCTAAATTAGAATTGTAAGTATGCAGAAGACTTTGCTCAG830.14219146165969984No Hit
AACACTGGGGATAAAAAGTGGTGTGTGTGCGTGCATATGTGCCTGTGTACTT820.14047831151922155No Hit
GACGCAAGGGCACCCTCTGCAATGACCTTGTCAACTCAGCCCAGGGATTTGT820.14047831151922155No Hit
GTGAGTGGGGAAAGATATCTGCTTCCTGCCAGGCTGGATAACTGTTGAATGG820.14047831151922155No Hit
GCTCTAGGGACCTAAATGCAGTGTGGGGTCATTGCCTTGCTTTGCGATGACA820.14047831151922155No Hit
TACAACGGGATAAAAATTAAATTAAGTTAATATCTAAAAGAAATAATGAAGT810.13876516137874323No Hit
ATATGGGGGGCAATAACATATTTTAAAGACTTCTGGATAAAAATGCTAATTT810.13876516137874323No Hit
TTTACCGGCAGAACCATTTGGAAAAATGAAATTTAGTAGTTCCAAGTTTCAA810.13876516137874323No Hit
TGAGTTGGGGCAGAGATGGTTTTTTAATGAAATCCCACCATCTATCTGAGTG810.13876516137874323No Hit
TCTTCAGGGACAGCATGAAAAAGCATATTTGTGTTTGAAAATATACAAGAAT800.13705201123826494No Hit
TTCGCTGGGACATTCCTTCTGCAGACCGGCACAAGAGCAAGCTGATTGCAGG800.13705201123826494No Hit
ACAAATGGGGGATGAACCTCAACACTATCGTGCTAAGTGGAAGAGAAAAAAA790.1353388610977866No Hit
TTTTGTGGGGTATGACCTTGTGAAATACATTACTTATTCATTTTATATCAGT790.1353388610977866No Hit
CATTACGGGAACGCATGTTTCAAAGGCACGTCTTTCCGTTCGTTGGTTTTCT790.1353388610977866No Hit
CACAAAGGGGTAACACCTAAAGTTGCCTGAAGAGCCAGTCATGTACCAGAGT780.1336257109573083No Hit
GGTGCAGGGACCTTTAACTTTTGGAAACTATTAGTCTTCATAAACTACCCCA780.1336257109573083No Hit
TTAGCTGGGCGGCCCTGGAGCTGCCCCTCCCCAGCGTGCCCGCCCCTGCCCC780.1336257109573083No Hit
CACCTGGGGACATCCGCTGACAAATGCATATATAGTGCCAATGTCATACTTG780.1336257109573083No Hit
CAAATAGGGGTTCCCTCCAGGCGAGCACGAAGACCTTCTCTTAGAGTCCCAC770.13191256081682998No Hit
TGGGAGGGGACATTTCATATTTATAATGTGCTGAAGGTACCATATTTTAAAT770.13191256081682998No Hit
GTAGAGGGGAGACTATGGGGTTTGTTCCTTTAACCGATACTGTAGGAAGGAC770.13191256081682998No Hit
CTACGAGGGATGCTAATTTACACCAGCACTTCCAATAACAACTAACTATTCT760.13019941067635168No Hit
TTAGCATGGGATCATTTACAATTTTGATTTCATTTCAACCATTTAACTGCAT760.13019941067635168No Hit
ATTTATGGGGAAACAGAATAGAGAGCTCCCAAGCAAATCTCTACAAATTTAA760.13019941067635168No Hit
GAGCTGGGGGTGTGGTTCCAACATTGTATGTGACAAAATAATTTGCCAAGTT750.12848626053587336No Hit
TTTATGGGGGATAATTAGAGCTAATTTGCAAGTACTGATGATAGGCTCATTC750.12848626053587336No Hit
TTGTTAGGGGATAATTTGTAAATGCTTTAGAGTTTTTTTAATTAACACTTGT750.12848626053587336No Hit
AGATGTGGGAGACACTAAAAAACATTATTTCACCTCCGTGGGATTTCAGGGA740.12677311039539504No Hit
ACATCAGGGATCTATGGCTTTGTTCTTTGTCAGTATCTTTTTGTTCTCAAAC730.12505996025491675No Hit
CTCGTTGGGGTTTTTTTAAAAGGGTCAGAATGGTTAACACTCAACCCTTTTT730.12505996025491675No Hit
TGTCACCGGGTCTTTAGACGGGATCTACAGAGGACTGGTTCCAGGACTCCCA730.12505996025491675No Hit
AGTATGGGGGTTGGCATATTAATGGATCCACACAATCAAATAGATTAGAAAT730.12505996025491675No Hit
AAACATGGGGATCTTGAACAGGGGCTGAAAATACAGAGAGAAAGCAAAATCA730.12505996025491675No Hit
CTGACCGGGATCCCACAATTTAGGTTTTTACCATATGTTGGTATGGTCACCA730.12505996025491675No Hit
AAAACCGGGGTGAGAAGATAGAGGCAGAACTGCAGGACATCTGCAATGATGT730.12505996025491675No Hit
TTTGGTAGGGAGTCTCAAAATACCTTCTCACAAAATGCTTATCAACTAAAGT730.12505996025491675No Hit
TCATGTGGGATTTTCATTTTTGCTCAATTTTAGAACTAATGGGAACTTTAAA720.12334681011443843No Hit
CTAACAGGGGACTGTTTCTTTTTAAGTTACATGTATATTTACCTGACCAATT720.12334681011443843No Hit
GTGTCAGGGCAATTTAAAAATACTCTTTTAAAACTTAGATCCTTTTATAGTT710.12163365997396013No Hit
CTCGTAGGGTCTGTCTCAAAAAATAAATAAAATAAAAAAGAGAGAAAGCTAG710.12163365997396013No Hit
GCCGGCGGGGGGCCAGACCACGGGAAGGACGGTGTTTGTGTCTGAGGGAGCT700.1199205098334818No Hit
TATCCTGGGATAGGAAGCTCTTCTAGGGGGTAATGGAAAAAGGGTTTTGAGG700.1199205098334818No Hit
GCTACCGGGGAGAACCTGTGAATGTGACCTATTTGGAAAATTCTATCTTTGC700.1199205098334818No Hit
GTCAGCGGGGACCTTGAGCACTTCGTTAAGGTGATGTCTGTCAGGTGTCTTC700.1199205098334818No Hit
GCAAATGGGAGTCATAGGCTGCAAGTCTATAAGATAATTCCATTGGGGAGGG700.1199205098334818No Hit
TTCACTGGGACCCCTGAACTTAAAAGTTATTTTAAAAAAGGCCTCCTTTGGT700.1199205098334818No Hit
GTTTACGGGGAGTTGCAACTATGCTGGCAACTGGAGGCAACAGAAAGAAATG700.1199205098334818No Hit
TGTTGCTGGGACCTTGACTCCGTGGTGACTTCATCTTTTTCATGAGTGTCAT690.1182073596930035No Hit
GCTAAGGGGGAAGTCTGTGTCTGTGCACTTCCCACAGACTGGAGTTTTTGGT690.1182073596930035No Hit
ACATACGGGTAGCCATAAACTGATTTCGATGGTAAGCCATGAGAAAGCATCA690.1182073596930035No Hit
TATAAGGGAGATGGTTGGGGGAAGAAGATCAAAAGAATGTTTTGTGACCCGT680.11649420955252518No Hit
CGCGGGGGGATTAGAGGTTTGGGCATATAACCAGATTAAAGTGAAGGAACTT680.11649420955252518No Hit
AATACAGGGGACGCTCTTCCCATCTGACAACGGGAATCTGCTTGGTGTTCTA680.11649420955252518No Hit
CACAGGGGAATGAGTAAATACGTGAGTATTTATTTTTATTTTTACTATTATT670.11478105941204687No Hit
TAATAAGGGGAAAACAGACTTTATTGTTAAAAAGGGAATTTGAGGCTTGGTA670.11478105941204687No Hit
GTTGCGGGGGTTTAAAGTGCTGAAGGAAATTTATCCTAGAATAGCAAATCTG670.11478105941204687No Hit
TATTGAGGGTCACACAGATTTTGAAAAGGGATTCATTATGGCTGAAGTAATG670.11478105941204687No Hit
GATGTTGGGAGACTCATGAGGGATCCCAATACCAGTTCCTATGTTGTCAAAA670.11478105941204687No Hit
AAACTGGGTCCACTTACTTACATGATCATTTCGAGAGATGTAATGTTGATTG670.11478105941204687No Hit
GGCCAAGGGACTCTTACCTTTCTGTCCTTGATAAAATAGTAAAGAAAACCAA670.11478105941204687No Hit
GTTTAGGGGATTTTCACATATTTTGAGCATCAAGATACTGGTCATTTTAAAA670.11478105941204687No Hit
GCTGATGGGGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAGATAGAGA660.11306790927156857No Hit
TAAATAGGGATGGAGAGGTTAAACAAGGAAAAACATTGCAAAACAATGAAAA660.11306790927156857No Hit
GATCATGGGCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAA650.11135475913109025No Hit
ACATTCGGGAAATGTCTTCTTTTGAGAAGTGTATGTTCATATCCTTCGCCCA650.11135475913109025No Hit
TCGCCAGGGAGCACCCATTTTTGAGAAAGCTGGTTCTACATGGGGGGATAGC650.11135475913109025No Hit
GGCAAAGGGGTCCTCTCAAAGTGCTGTACGCGAGCTCGGCGCCACCTCCGCC650.11135475913109025No Hit
ATTTCAGGGATACAAATCTCAGTTATTTGGGAGCTAACCTCAAATGCAACTA650.11135475913109025No Hit
GTTAGAGGCCGATCTTTGAGTGGGAGGAGAATAAACTAATTAAAGCAGGAAT640.10964160899061194No Hit
CTCATGGGAGATAAACTAATCAAATAAATTAGAATAATGTGACAACATTACA640.10964160899061194No Hit
TTGCGGGATACAAGAAGAGGCCGGGCACGGTGGCACACGCCTGTAATCCCAG640.10964160899061194No Hit
CTGGCTGGGAGTAAGGAGCATTTAGCAATGATGGAAAGGATTCTTGGACCTC640.10964160899061194No Hit
AGCGATGGGAGCTGCAATTCTCATTGCAAGATCATCGCCTCCCTGGTTTATA640.10964160899061194No Hit
TTTATAGGGTCTGCCTTGTCTGTGTGTGAAGTTCATACCCGGGGTAGATATG640.10964160899061194No Hit
CTGGATGGGGCATCAAAATGGTTTCCAGGACAACACCTTATGAACCAAAGAG640.10964160899061194No Hit
CTACATCCGGGGGGATGAAAGGTGCCCTAAGCAGGAGAAACTGAACAAAAGG640.10964160899061194No Hit
GAATAGGGGGTGTGTTTTCTGGGAGCATCGAAGCTCTGTTTCGGAAGAGCTG640.10964160899061194No Hit
TCACATGGGATTGTGTGCTTTATTTCTATGATTATTACATTGTAATATATAA640.10964160899061194No Hit
CCGAATGGGAGGAGACGACAGCTCTGTGCCTGTTATTCTAGAACAAGCCATC640.10964160899061194No Hit
CCACCAGGGATTACAGTTTTTGGTGAAATTGATGAAGATGGATTTTATTATG640.10964160899061194No Hit
TGCATGGGATAGGTACCTTGTACCTAATTTTATATTCATAATTTTGTATATT640.10964160899061194No Hit
CTCAACGGGTGGCTGCATTGAATGATATACGGTATCAGAGACTGGTGGTGGA630.10792845885013362No Hit
CATCGCGGAGTGTTCAGATTTAATTGAAGTCTCATTTTAGGGTCATGCTGCA630.10792845885013362No Hit
ATTAAAGGGCAACTTCCTTTCATGTTCCCTATATATCATCTAACCCAAACTT630.10792845885013362No Hit
CAAGCCGGGCACCTTCTATCAGAATAGCAAAAGAAGCTGCTTTCATAAACTG630.10792845885013362No Hit
TCTAAGGGAGTAAGGAGACAGTGAGAATGAAGAATGAAAGAAACCCATGGAT630.10792845885013362No Hit
TACAGAGGGACCAAAAGGTAACTCTTTCTAGAATCTGGCACACCATTTAATA630.10792845885013362No Hit
GTAGACGGGGTGCTGTCCTCTACTTGCCCTGTCACCTCCAGCATCTTGAACA630.10792845885013362No Hit
CGTACGGGGGCCGCAAGCCCTTCCCTGGGGAGTACCTGATGGCTGCCCTTTG630.10792845885013362No Hit
GTTACAGGGAAGAAAAGAAACACTGGTAGTAGAAAGCTGAGTCCTAAAATAT620.10621530870965532No Hit
CATTAGGGAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGATT620.10621530870965532No Hit
GCTTAAGGGACATATCCTCTGTGACTGAGCATTGCAGTTAGTTAACTTAAAG620.10621530870965532No Hit
TCATCCTGGGATGAATTAAAGAATCCAGATAGGTAGCACCTTATCCTTATTC620.10621530870965532No Hit
AACACAGGGTGAAGTTACAATTATATGTATGTATATGTATGTGTGATTTATA620.10621530870965532No Hit
TTCGAAGACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG620.10621530870965532No Hit
CAAAGAGGGGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAAGCCTAGT620.10621530870965532No Hit
ATATATGGGAGTCAGCAGTGGGCTATTGGTGGTTAAGTTTTGGGGGAGTCAA620.10621530870965532No Hit
CGTTTAGGGGTTTCAGGTTTTTCTGAGGAATTTTAAATACCCTGTGTCCTCT620.10621530870965532No Hit
ATACAGGGGTGAGTATAAATAGGGTTTTGTTTTGTTTTTTTAACCTAAAAAC620.10621530870965532No Hit
TATCTGGGGGAGCCAAAAGTCCATGAACCAGTTCGAATTGCCTATGACAGGC620.10621530870965532No Hit
CATGTCGGGATAAGTTTATATGTTTAATTTAAAAATAATGGCTGTATTCAAC610.104502158569177No Hit
TTTAAAGGGTTTTCTAAGTGGTTTTTCCCAATACTCTGAAACTAGGATTTAT610.104502158569177No Hit
GAAGATGGGATCAAGAAACTTTCCCCCTATGTTTCTTACAGAAATTTTATGG610.104502158569177No Hit
TTCTGTGGGATTATTGATGGACTGATAATTCCCAGGTCTCTTCTGCCAGCTC610.104502158569177No Hit
CAAAATGGGAACAGCATTCATAATAGCTGAGTGGTGGAAACAATCCTGCTGT610.104502158569177No Hit
ACCAAAGGGAGATCTACCATTTGATCCAGCAATCCCACTACTGGATCTACCA610.104502158569177No Hit
GAGCGGGGGGGCATATCAGATATGCAAGTCTGTGAATTTTTTGCACAGTAAT610.104502158569177No Hit
TTGCAGGGGCCAGGCCTTGTTCATCAAAACAGGTGACAGTGAGTAACCAAAT610.104502158569177No Hit
GCAACAGGGCTGTGTATCTATCTAGAGGAAAATAAGTCATTATACGAAAAAG610.104502158569177No Hit
TTCTGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA610.104502158569177No Hit
CGATACGGGAGCTTTTATCAGGCCAAGTTAAAGGCTGACTACATTTTTTCAT610.104502158569177No Hit
CTAGTTACGGGATGTACAGTTGGCCCTATGTATCCATGGGTACACATCTGTG610.104502158569177No Hit
GCACCAGGGAACTTATCAAAACATAGAATTAAGAAAAATAGCTGTATTGAGG610.104502158569177No Hit
AGGCATGGGATTCTAACTGGTGTGAGATGGTATCTCATTGTGGTTTTGATTT610.104502158569177No Hit
CTCAGCCGGGCCTCTTCACTTGTGTGTGTGTGTGTAGCGGAAAAGGACAAGA600.10278900842869869No Hit
CTAGAAGGGGTAGGGTTTTCATAGTGGACAAAGAACTTGTGGTCTTTTAAAA600.10278900842869869No Hit
AAAGTTGGGGAGTTTTAATTTTAAGGTATACTTGTAGGTGGATGATGAAGAC600.10278900842869869No Hit
CCCGGTGGGAGTCAAATTTAGATAACAGGTTAATATGACATGTAATTCTGCA600.10278900842869869No Hit
TGTACGGGGATTCTTGAATCACTGCATTCATGTAGAGTAGTTGTTGGATGTG600.10278900842869869No Hit
ACACTAGGGATTCCAGAGCTGATGTGATGGGCTGTGCAGAAGGGGGCTGTAT600.10278900842869869No Hit
GTTTTGGGGTCCTCTCACATGGTGGCTTTAGGAAGATCCTTGGCCAGGAGGG600.10278900842869869No Hit
GTAATAGGGAGTTGCATTCTGGCCACTTTGACCTTATAGCTCCCAAATGATG600.10278900842869869No Hit
TATACGGGAGCCACTGTGCCTGGCTGTGTTTTTAATAAATAGCAAAAGAAGC600.10278900842869869No Hit
AACGCTGGGGGAGATAAAATGATGAGATGAAATGATGAGAAGAAATGAGATG590.10107585828822037No Hit
CGTCAGGGGTGACTTGTTCACTTTGTGTTTGTCCAGCTTCCATGGCATGTGG590.10107585828822037No Hit
CGCTATGGGGACCATTGTGAAATCCTTTCTCAGTGTAACTGCATTTCTAATA590.10107585828822037No Hit
GCTCATGGGATAAGTTTCTCTTTAAATAAGCACATCATTCTGTCTTCCTTAA590.10107585828822037No Hit
CTAGCAGGGACTAACAAAAGTGCCTCATCATAAAAATACAGTTGGTTTTTAG590.10107585828822037No Hit
GGGACAGGGAGGCTGTTCCTTGTACTCTTAGGATATTTGAGTCACTTGTGTC590.10107585828822037No Hit
GTAAAGGCAGGGCTATAGGATATGAACTTCACAATTCATTTGGGTACATTCT590.10107585828822037No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)