FastQCFastQC Report
Sat 31 Dec 2022
SRR5849824.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849824.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73283
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGCCGTCT6470.8828787031098618RNA PCR Primer, Index 36 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGC6240.8514935251013196No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCTTATAC1210.16511332778406998No Hit
AATAAGGGGTGTTTTCAGTCAAAAACTGGAAACAATCCAATGTTCGTCAGCA1000.13645729568931403No Hit
ACATTTGGGAGCCACAGTCTTATGGCCCTGATGAAAGGACAGAGCCAGTTAG870.1187178472497032No Hit
TAAGTGGGGGCTGTTGATGAATTAAGAGACAGAAAAATTCTGATACGATTCA850.11598870133591693No Hit
TACCAAGGGATACAAGGCACCAAACTACTAGATTTGGCATTAAAACAAATGT850.11598870133591693No Hit
TTGCCTCGGGATGGTGGTACCAGGAGACGATAAAGTAGAAAGTTGGAGTCAA830.11325955542213063No Hit
CTTTCTGGGCGCCCCAGCTCGAGAAGGAAGACAGAAGTAGGAGCGAGGCATC800.10916583655145122No Hit
GCGCTAGGGAGGGTGAGCATCAAACTCAAACTACGCCCTGATCGGCGCACTG790.10780126359455809No Hit
CCTATAGGGAAAGAGCTTCTGCACAGCAAAAGAAACTACCAGCAGAGTAAAT770.1050721176807718No Hit
TTCAAAGGGATGCCTTTGTCAGCAGCATGGAAACAGACTAATACATCAACAG750.10234297176698551No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)