FastQCFastQC Report
Sat 31 Dec 2022
SRR5849823.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849823.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72456
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGCCGTCT6450.8901954289499834RNA PCR Primer, Index 36 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGC6290.8681130617202165No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCTTATAC1310.18079938169371756No Hit
CCGTGAGGGAAATGCGGGGAAGAAAGATTGCAAATAAATACATGGGCCAAAG880.12145301976371867No Hit
AATAAGGGGTGTTTTCAGTCAAAAACTGGAAACAATCCAATGTTCGTCAGCA860.11869272385999778No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCT840.11593242795627692No Hit
GATGTAGGGACTAGACAGATCATCAAGACAGAAAGTCAACAAAGAAACAATG830.11455228000441647No Hit
TTCTAGGGGAGACACTAGGCCCAAAGGCTAAGTGAAGTATGTTTTTCAAGAC830.11455228000441647No Hit
GCTTAGGGGAAGCATCTATTGGTCATATACCACATACCAGGCCATGAGTTTA820.11317213205255604No Hit
TAAGTGGGGGCTGTTGATGAATTAAGAGACAGAAAAATTCTGATACGATTCA800.11041183614883515No Hit
ATATTTGGGACCATTTGATTTGAAGACAGTCTTGACAAAGGAATCACAGGAA790.10903168819697473No Hit
GACAATGGGAATTAAAATAATGGCAAGGGCTGCAATTATTTTGCATCAACCT790.10903168819697473No Hit
TTTAGTGGGTAAAAAAGCTCATCATCACTGGTCATTAGAGAAATGTAAATCA760.1048912443413934No Hit
CCTATAGGGAAAGAGCTTCTGCACAGCAAAAGAAACTACCAGCAGAGTAAAT750.10351109638953296No Hit
TTCACTGGGAGGCTTAACTTCTACAGAAAATAAACAGGGTGATCATAATTAC750.10351109638953296No Hit
TACCAAGGGATACAAGGCACCAAACTACTAGATTTGGCATTAAAACAAATGT750.10351109638953296No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)