FastQCFastQC Report
Sat 31 Dec 2022
SRR5849822.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849822.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72897
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGCCGTCT6540.8971562615745504RNA PCR Primer, Index 36 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGC5800.7956431677572465No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCTTATAC870.11934647516358698No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCT810.11111568377299477No Hit
TTTAGTGGGTAAAAAAGCTCATCATCACTGGTCATTAGAGAAATGTAAATCA800.10974388520789609No Hit
TTCTAGGGGAGACACTAGGCCCAAAGGCTAAGTGAAGTATGTTTTTCAAGAC780.10700028807769868No Hit
CGCTGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC780.10700028807769868No Hit
CCGTGAGGGAAATGCGGGGAAGAAAGATTGCAAATAAATACATGGGCCAAAG770.10562848951259998No Hit
GGTGCTGGGAAAGAAGAAATAGATCATTTAGGCTTCTTCAGTAATCCAGGCA750.10288489238240257No Hit
ACATTTGGGAGCCACAGTCTTATGGCCCTGATGAAAGGACAGAGCCAGTTAG750.10288489238240257No Hit
AGATTGGGACTCATATAACAGTATGGATGAATCTCAAAAACATTAAATGAAA730.10014129525220516No Hit
TGACATGGGGGTGTGGTTTTAGACGAATTGATTCCAGATTGCACACACAGGA730.10014129525220516No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)