FastQCFastQC Report
Sat 31 Dec 2022
SRR5849817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48449
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGCCGTCT3300.6811286094656237RNA PCR Primer, Index 36 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGC2560.5283906788581808No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCTTATAC820.16925013932176103No Hit
TCGTGCGGGATTTGATCTTGTTTCAGCTAAACTAAATGTGTCTGTTAAGATT640.1320976697145452No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGT630.13003364362525544No Hit
GCTCGGGAGGCCCTTCAAAGATATTAGTATTTTGGTTTAGAATGAACTCTTG610.12590559144667587No Hit
CTCGTTGGGGTGACCAAGATTTTATTTTACTTTACATCACAGTAAATTGTTT530.10939338273235773No Hit
TAGCGAGGGATCCTATAATGCATTATAAAAATAAATCATTTTTCCTAAATCA490.10113727837519866No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)