FastQCFastQC Report
Sat 31 Dec 2022
SRR5849816.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849816.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48142
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGCCGTCT3800.789331560799302RNA PCR Primer, Index 36 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGC2600.5400689626521541No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCTTATAC640.1329400523451456No Hit
TATAAAGGGCTATTCACAATGGCAAAGACTTGGAACCAACCCAAGTGTCCAA630.13086286402725272No Hit
TCGTGCGGGATTTGATCTTGTTTCAGCTAAACTAAATGTGTCTGTTAAGATT560.11632254580200242No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)