FastQCFastQC Report
Sat 31 Dec 2022
SRR5849812.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849812.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68921
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGCCGTCT7171.040321527546031RNA PCR Primer, Index 36 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGC6670.9677746985679256No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCTTATAC970.14074084821752442No Hit
ACATTAGGGAAGATGTCCTCTGTGAAGTTGCGGCTGCTCTGCAGCCAGGTAC870.12623148242190332No Hit
GTCTTGGGGGGCTGTGAGAACCTGCCTCACATGTAGCTGACCCCTTCCTCAC860.12478054584234123No Hit
TAGCTGAGGGAAGACACATAATCCTCAGATTTTCCAAGGTTGAAATGAAGGA820.1189767995240928No Hit
CTTACCGGGCTGTCATCTCTGATTTCTTTGAGCAGTGGTTTCTAGCTCTCCT780.11317305320584438No Hit
GAAACAGGGGCAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAGG760.11027118004672015No Hit
TCGCTTGGGAGGCTCTTCTTTGGTTCCATATGAACTTTAAAGTAGTTTTTTC760.11027118004672015No Hit
AGACTCGGGACAATGTGGTAGGCTGAAACGATGGTGTTAATTCTTAGGACTA740.10736930688759595No Hit
GTAGAAGGGAGTTTCCCATCGCCTCTGTGGAATGTGTCTGTGTTTCTCTCCG730.10591837030803383No Hit
TTTCCGGGGAGCCAGGACAAATTGGGAAGAGTATATGCAGTAATATGTGACC730.10591837030803383No Hit
GGTGCTGGGGAGCTGCTCTAGCAAATTAATCAAATTCAAGGAGGGGGTCATG710.10301649714890963No Hit
GCTATTGGGCAGTTGGGATAGGCTTGTTTTGGCTGTTGAAAATGTTTCTAGT700.1015655605693475No Hit
TGGATTCGGGGAGGGCCTTGGCACCTCTTGCATCCAGGCAGTCTTGTGAGAT690.1001146239897854No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)