FastQCFastQC Report
Sat 31 Dec 2022
SRR5849811.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849811.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68597
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGCCGTCT7591.1064623817368107RNA PCR Primer, Index 36 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGC6961.0146216306835576No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCTTATAC1060.15452570812134642No Hit
TAATAGGGGGGGGGCCGGGGGAGAATGGGGACGGTGTCACGATGCTGCGACT830.12099654503841277No Hit
AGAGCAGGGACGAGATCAGGAGATCGAGACCATCCTGGCTAACACGGTGAAA800.1166231759406388No Hit
TCGTGATGGGATTCTTAAGGCAAATCCATGGGGGGAAGATACTGGACTAAAG790.11516538624138083No Hit
TAGCTGAGGGAAGACACATAATCCTCAGATTTTCCAAGGTTGAAATGAAGGA780.11370759654212284No Hit
TTTGCTGGGATATTAGCTATTTAAAATAATTCTTCAAGAGCTAAAGAAAACC770.11224980684286485No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCT770.11224980684286485No Hit
GTCTTGGGGGGCTGTGAGAACCTGCCTCACATGTAGCTGACCCCTTCCTCAC720.10496085834657493No Hit
TCGCGAGGGAGAGATTTCAGAGGGGAATGGAAAAACAGTTCTAATCAATAAG710.10350306864731695No Hit
GCATTTGGGCCAGTGCCAGTACCCCCTCAAGTTTTTATCAGTTAGCACTTGG710.10350306864731695No Hit
GCTATTGGGCAGTTGGGATAGGCTTGTTTTGGCTGTTGAAAATGTTTCTAGT700.10204527894805894No Hit
TATGCTGGGACTTCCATAGAAGAACATCACTTTTAACCTTGCTTTGGCGAAG690.10058748924880097No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)