FastQCFastQC Report
Sat 31 Dec 2022
SRR5849810.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849810.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67833
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGCCGTCT7411.0923886603865376RNA PCR Primer, Index 36 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGC6370.9390709536656199No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCTTATAC870.12825615850692143No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCT820.12088511491456959No Hit
TCGCGAGGGAGAGATTTCAGAGGGGAATGGAAAAACAGTTCTAATCAATAAG730.10761723644833636No Hit
TAGCTGAGGGAAGACACATAATCCTCAGATTTTCCAAGGTTGAAATGAAGGA720.10614302772986599No Hit
TATGCTGGGACTTCCATAGAAGAACATCACTTTTAACCTTGCTTTGGCGAAG720.10614302772986599No Hit
TTGTATGGGGCAACCCCAGGTGCACACCACATCTAAGTATCAGAGACCAAGG710.10466881901139563No Hit
TGGATTCGGGGAGGGCCTTGGCACCTCTTGCATCCAGGCAGTCTTGTGAGAT690.10172040157445492No Hit
TCTAGGGGGAGGGGCTTATAATGTATGATGTTTGCCTCATTAGTGTGACATT690.10172040157445492No Hit
TCGCTTGGGAGGCTCTTCTTTGGTTCCATATGAACTTTAAAGTAGTTTTTTC690.10172040157445492No Hit
TACACCGGGAATAGAAGTGGAATTAGACAACATTTGTCATTTTTACATCTGG680.10024619285598454No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAAACTCGTATGC680.10024619285598454No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)