FastQCFastQC Report
Sat 31 Dec 2022
SRR5849806.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849806.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48904
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGCCGTCT6081.2432520857189595RNA PCR Primer, Index 36 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGC5321.0878455750040896No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCTTATAC1080.2208408310158678No Hit
TAGTGTGGGAACTACAAGAAATATTTGTGATGTTCTCAACACAAAAGAGATA750.15336168820546378No Hit
TATCCCGGGATGACAAACATACGAAAAACACACCCATTATTTAAAATTATTA700.14313757565843285No Hit
TAGTCAGGGGGAAAATTCTCAGCCATTATTACTTAAATATTTTCTTTTTCTT700.14313757565843285No Hit
AAGTATGGGGCTCTTCCGATCTTTTGCAGGGATAACAACCAAACTCTACTGG680.13904793063962048No Hit
GGCCTGGTACGAAAGGATTGAAAAAGTGAAAGCAATAAATAAACATTAAAAT660.1349582856208081No Hit
GTATAGGGGATGTGGGAAAAGTTTTTCCCAGAAGTTATCCCTTACTGTACAT630.12882381809258958No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCT630.12882381809258958No Hit
TCGACTGGGAGGCACTCCTGAGGCATAAGAAATAAAAGATATATAAATAAGC630.12882381809258958No Hit
TCAGCCGGGAAGGAAAATGTGCAGTAAAAATCAATAATAGCAAACATTTGTT620.1267789955831834No Hit
GCTGCGGGGGACATTTGCGCAGAATTTTTAGACATAAAGAAATGCTTGCATA610.12473417307377721No Hit
AAAGTGGGTGTAAGTGAAAGTGCAAGGGTTAGATTTGACTTTAAGCACATAC590.12064452805496481No Hit
CGGGACGGGTATTCTCTGAAAAAAAGTTTCATGAAGAGAGTTCAGTTCACAG570.11655488303615245No Hit
AATTTTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA570.11655488303615245No Hit
ATTTATGGGGAAGGAAAAAAATAATGAAAAAAATATATGGGTTGAAAACATT570.11655488303615245No Hit
GTACGCGGGATTGCCTCATGTTACCTACCTGCTATTTTCACTGTAATTTCAT560.11451006052674627No Hit
GCTTTGGGGATTCTCTTTCCTTCAGGAAATGAACCCTTCTTACATCATTCAA560.11451006052674627No Hit
TGCACAGGGGTCATAACACCAGAAAAAAATCACCTTTACTAAGAGGAAGACA560.11451006052674627No Hit
GTCCTGGGGGGTTCTGATTCTAGCCTTGTGCTGCTTTTCAATTAGCCGAGTT550.11246523801734008No Hit
TTCGTTGGGTATTCTGGAAGACAAGAAGTTAGAAAACGAGGGAAACACAGAA550.11246523801734008No Hit
TAGGAGGGCAAATCAGTTCTCTAACCACAACGGTAAAGTATTAGAAATCAAT550.11246523801734008No Hit
GTAGGCGGGATCATCGGAGTCAGCCAGGAGCTGTCACCAAGGAAACGCTACC550.11246523801734008No Hit
CTCCACAGGGGATCCACGTTTTTGTTTTTTTAATGTTAAATGTGTAACTCAG540.1104204155079339No Hit
GCGTTGGGGGTGGTTAAGAATCTTACATACTTATCATTTTTTGTTGTTGTGA540.1104204155079339No Hit
GCATCAGGGAGCAGGCCTCGCACATCCAGGTGCTCTCGGACGACGTGTGCGC540.1104204155079339No Hit
TCGCATGGGGATAACACCATGACTCAACATGTTTGGGGTCTAAGTAATTAAA540.1104204155079339No Hit
ATACATGGGCAGCCGGCGACTTCATCGAGCATGCCGAGTTCTCGGGGAACCT530.10837559299852773No Hit
GAGTGCGGGGATGACCAGTTAAGCAATGTTATAGTTCCATGCTCCGATACTA530.10837559299852773No Hit
GATGGTGGGAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGG530.10837559299852773No Hit
TTAATAGGCACTCACGCTGATTGTTGAACAGATAAATACGAAACTGCCATCA520.10633077048912154No Hit
ATGAATGGGAGCTGTTAAAACTTCTAGATTGAAATTTGACAGCCAGGGTTAC520.10633077048912154No Hit
GAAGTTGGGCCTTCAAAAAATCAGTGAATCCAGGAGTTGATTTTTTGAAAAG520.10633077048912154No Hit
TGGTGGGGGGCAAAGGGAGACCTAGAACCTCAGAAATGACAACATCCACCTC520.10633077048912154No Hit
TACATCGGGTCGACAGTAAAGTTGAAATGGTGACGTCCACTGCTGGCTTTAT520.10633077048912154No Hit
GTTCCAGGCATTTATTACCATAACCATATTTAAATCAATGTGAATCTTACAT510.10428594797971535No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAAACTCGTATGC510.10428594797971535No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACA500.10224112547030917No Hit
AAGCACGGGAGAGATTAATACAACTACTTAAAAAATATAGTCAATAGGTTAC500.10224112547030917No Hit
GTTACGGGCAGGGTTCCGTCCTCAATCCACATGATCTTGCAAGAGTCAAGAT490.10019630296090298No Hit
ATCACTGGGATTCTTTCCTGTCACCATTATATGTCTTTATGAAGCAAGGGAG490.10019630296090298No Hit
TGTGCAGGGGTCTTCAGTCAAAGCTTTCACTCCTGTCCCTGTTCCACTCCTG490.10019630296090298No Hit
AGATGTGGGTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCA490.10019630296090298No Hit
ATTCAAGGGAAACAATGATAATTTTACTTACTCCTTTCCAATTTAAATGCCC490.10019630296090298No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)