FastQCFastQC Report
Sat 31 Dec 2022
SRR5849805.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849805.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48517
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGCCGTCT6371.3129418554321166RNA PCR Primer, Index 36 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGC4881.0058330069872417No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCTTATAC1140.23496918605849498No Hit
TCGACTGGGAGGCACTCCTGAGGCATAAGAAATAAAAGATATATAAATAAGC810.1669517900941938No Hit
TATCCCGGGATGACAAACATACGAAAAACACACCCATTATTTAAAATTATTA740.15252385761691778No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCT710.1463404579837995No Hit
TTCGTTGGGTATTCTGGAAGACAAGAAGTTAGAAAACGAGGGAAACACAGAA640.13191252550652346No Hit
GCATCAGGGAGCAGGCCTCGCACATCCAGGTGCTCTCGGACGACGTGTGCGC630.12985139229548406No Hit
GCTTTTGGGTTTCTAATAACGTCATGTAATTGGACCAAGGAAGAGTTGCTAA630.12985139229548406No Hit
TCGCATGGGGATAACACCATGACTCAACATGTTTGGGGTCTAAGTAATTAAA620.12779025908444464No Hit
GGCCTGGTACGAAAGGATTGAAAAAGTGAAAGCAATAAATAAACATTAAAAT610.1257291258734052No Hit
TTAGTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA610.1257291258734052No Hit
TACTGTGGGGTAGCATACAGCTTACCTGCTAGAATGTTCTTACCCTCTTACC600.12366799266236576No Hit
CTCTCAGGGATTTAAATTAGATTGGCAGAACAGATTTGAAAATTCAGTCCCT600.12366799266236576No Hit
AATTTTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA580.11954572624028689No Hit
GCTCTGGGGGTCTTTAAAGGATGAATTAGCATGGTCTTAAAATACATTTCTG580.11954572624028689No Hit
GCGTTGGGGGTGGTTAAGAATCTTACATACTTATCATTTTTTGTTGTTGTGA580.11954572624028689No Hit
TAGTGTGGGAACTACAAGAAATATTTGTGATGTTCTCAACACAAAAGAGATA580.11954572624028689No Hit
GTAGGCGGGATCATCGGAGTCAGCCAGGAGCTGTCACCAAGGAAACGCTACC580.11954572624028689No Hit
CGTTTAGGGCAGCTGAGAAGGCTACAAGTGATGAGAAAGATTCTGTTTCAAA570.11748459302924749No Hit
AAGTATGGGGCTCTTCCGATCTTTTGCAGGGATAACAACCAAACTCTACTGG570.11748459302924749No Hit
TAGGAGGGCAAATCAGTTCTCTAACCACAACGGTAAAGTATTAGAAATCAAT570.11748459302924749No Hit
ATCACTGGGATTCTTTCCTGTCACCATTATATGTCTTTATGAAGCAAGGGAG560.11542345981820805No Hit
ACCTGGGGACAGATTAAATCTAAGTATTACAATAAAATATTAAATATGTACA560.11542345981820805No Hit
TGATGAGGGGAGTATGAGCAAGGCACGGGCTGCTGGCAGGGCCCCAACCGCT550.11336232660716862No Hit
GCTGCGGGGGACATTTGCGCAGAATTTTTAGACATAAAGAAATGCTTGCATA550.11336232660716862No Hit
TGCACAGGGGTCATAACACCAGAAAAAAATCACCTTTACTAAGAGGAAGACA550.11336232660716862No Hit
TCAGCCGGGAAGGAAAATGTGCAGTAAAAATCAATAATAGCAAACATTTGTT550.11336232660716862No Hit
ATACATGGGCAGCCGGCGACTTCATCGAGCATGCCGAGTTCTCGGGGAACCT540.11130119339612919No Hit
CTGCGGGACTTGTTGACTCTTTCAAACTTAAGTTAATGATACAGTCAGGACT540.11130119339612919No Hit
ATTTATGGGGAAGGAAAAAAATAATGAAAAAAATATATGGGTTGAAAACATT540.11130119339612919No Hit
GGGTGCAGGGATCACATTTTTTGAATGTTCATATTTTCTGGCATCTCAAGAA540.11130119339612919No Hit
GAAGTTGGGCCTTCAAAAAATCAGTGAATCCAGGAGTTGATTTTTTGAAAAG540.11130119339612919No Hit
TAACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG540.11130119339612919No Hit
GCGTACGGGAAGTCTTTCTCCATTAAGAATGACGTTAGTTATAGGTTTTTGT530.10924006018508975No Hit
AATTGAGGGCAGACAAAGTAAAATAAAAATTTAAGTAACTAAAACACAATTC520.10717892697405032No Hit
ATTCCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC520.10717892697405032No Hit
AAAGTGGGTGTAAGTGAAAGTGCAAGGGTTAGATTTGACTTTAAGCACATAC520.10717892697405032No Hit
GTCCTGGGGGGTTCTGATTCTAGCCTTGTGCTGCTTTTCAATTAGCCGAGTT520.10717892697405032No Hit
ACGAGTGGGGGAATCCCAATGGTTAAGAAAAAGGGAGTGAGTAATAGTCAAG520.10717892697405032No Hit
CATAACGGGACAACTAAGGTAAAGGCAAACTCAGTAAAGCAGGAGTTTGAAA520.10717892697405032No Hit
CAACTGGGGAGGAAACACTGTATAGCTTAGTAGCCTCTTAGAGATGAAATTA520.10717892697405032No Hit
TATATGGGGAGAACTAAATATCCTGGTTGAGCTTTGAAAGAGCCCTGTCGGC520.10717892697405032No Hit
CATCAGGGAGGTTGCATGCTCCTTATAAGAAACTAACTAATGCCTGATGATC510.1051177937630109No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAAACTCGTATGC510.1051177937630109No Hit
TTGTTCCGGGGGACCCCAAACCTCAGCATCACATAATATACTCATGTAACAA500.10305666055197148No Hit
CGGGACGGGTATTCTCTGAAAAAAAGTTTCATGAAGAGAGTTCAGTTCACAG500.10305666055197148No Hit
CTCCACAGGGGATCCACGTTTTTGTTTTTTTAATGTTAAATGTGTAACTCAG500.10305666055197148No Hit
CTTGCAGTGGTACACTTTGGGGGATCCAAAAGGAGCTGCAATTTTAAAGTCT500.10305666055197148No Hit
TACCTTGGGCTTCCGATCTTTTGGACGGGACTATTTGAAACCCAGCAATCAC500.10305666055197148No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACA500.10305666055197148No Hit
GAATATGGGGGCTTGTGGGTCACTTGTTGTGCAAAAGTGAATTAATTTCAAA500.10305666055197148No Hit
TGTAGGGCTTGTCTGTGTCTGGGAAGGGATGGCCAAGGCCGCTAGGGTCCTT500.10305666055197148No Hit
TGACCTGGGAATTTCCCTGCCCACAAAGGCATAAACTTGAAAACACATGAGA490.10099552734093205No Hit
ATTTTTGGGCTGTTACATTATTTTCTCAAAAGGAACACATGTTTACTACAAA490.10099552734093205No Hit
ATCTAAGGGAGCTTTAAATTCTGTGGCTTATGAAAGGAGTGCAATGCAGAAT490.10099552734093205No Hit
CTTAATGGGTGGTGTCAACCCATTCTCAAGATGGGACAATAACTCAAGATAA490.10099552734093205No Hit
AATAGTGGGGTATTTTTATAGCTTGTTAAAGACATGGCTAGTTGCATTTGTA490.10099552734093205No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)