FastQCFastQC Report
Sat 31 Dec 2022
SRR5849804.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849804.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48010
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGCCGTCT5801.2080816496563216RNA PCR Primer, Index 36 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGC4951.0310352009997918No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCTTATAC900.18746094563632576No Hit
TAGTGTGGGAACTACAAGAAATATTTGTGATGTTCTCAACACAAAAGAGATA770.1603832534888565No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCT760.15830035409289733No Hit
TCGACTGGGAGGCACTCCTGAGGCATAAGAAATAAAAGATATATAAATAAGC750.15621745469693815No Hit
TTCGTTGGGTATTCTGGAAGACAAGAAGTTAGAAAACGAGGGAAACACAGAA710.14788585711310145No Hit
TATCCCGGGATGACAAACATACGAAAAACACACCCATTATTTAAAATTATTA640.1333055613413872No Hit
TGCACAGGGGTCATAACACCAGAAAAAAATCACCTTTACTAAGAGGAAGACA630.13122266194542803No Hit
TACTGTGGGGTAGCATACAGCTTACCTGCTAGAATGTTCTTACCCTCTTACC620.12913976254946888No Hit
GTCAAAGGGGTGTAAATGAACACATAGATCTTATAATTTATGTGAAATTGTA600.12497396375755052No Hit
GCGTACGGGAAGTCTTTCTCCATTAAGAATGACGTTAGTTATAGGTTTTTGT600.12497396375755052No Hit
TCAGCCGGGAAGGAAAATGTGCAGTAAAAATCAATAATAGCAAACATTTGTT590.12289106436159133No Hit
ATACATGGGCAGCCGGCGACTTCATCGAGCATGCCGAGTTCTCGGGGAACCT580.12080816496563215No Hit
GGCCTGGTACGAAAGGATTGAAAAAGTGAAAGCAATAAATAAACATTAAAAT580.12080816496563215No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACA580.12080816496563215No Hit
ATTTTTGGGCTGTTACATTATTTTCTCAAAAGGAACACATGTTTACTACAAA570.118725265569673No Hit
GCGTTGGGGGTGGTTAAGAATCTTACATACTTATCATTTTTTGTTGTTGTGA570.118725265569673No Hit
TCGCATGGGGATAACACCATGACTCAACATGTTTGGGGTCTAAGTAATTAAA570.118725265569673No Hit
CTGCGGGACTTGTTGACTCTTTCAAACTTAAGTTAATGATACAGTCAGGACT560.11664236617371382No Hit
ATATTTGGGATTCCTTAGCTGGGCTGGTAGAATTGAAAAGTCTTTCTCAACT550.11455946677775464No Hit
AAAGTGGGTGTAAGTGAAAGTGCAAGGGTTAGATTTGACTTTAAGCACATAC550.11455946677775464No Hit
TGGTGGGGGGCAAAGGGAGACCTAGAACCTCAGAAATGACAACATCCACCTC550.11455946677775464No Hit
CGTCATGGGCTCAAAAATGCATTTGAATTTCATAGAGGTTCTCATTAAAACT540.11247656738179546No Hit
CTTGCAGTGGTACACTTTGGGGGATCCAAAAGGAGCTGCAATTTTAAAGTCT540.11247656738179546No Hit
AATTTTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA540.11247656738179546No Hit
GTATAGGGGATGTGGGAAAAGTTTTTCCCAGAAGTTATCCCTTACTGTACAT540.11247656738179546No Hit
TAGGAGGGCAAATCAGTTCTCTAACCACAACGGTAAAGTATTAGAAATCAAT540.11247656738179546No Hit
GCTTTTGGGTTTCTAATAACGTCATGTAATTGGACCAAGGAAGAGTTGCTAA540.11247656738179546No Hit
TTAGTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA530.11039366798583627No Hit
CGACAGGGGTATTTAAAAGAAAATTGAGAGAAAGGACTACAGAGCCCCGAAT530.11039366798583627No Hit
GAGTGCGGGGATGACCAGTTAAGCAATGTTATAGTTCCATGCTCCGATACTA530.11039366798583627No Hit
GCTGCGGGGGACATTTGCGCAGAATTTTTAGACATAAAGAAATGCTTGCATA530.11039366798583627No Hit
GTACGCGGGATTGCCTCATGTTACCTACCTGCTATTTTCACTGTAATTTCAT530.11039366798583627No Hit
GATGGTGGGAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGG530.11039366798583627No Hit
ATAGTTGGGGAGCACACTTTAACCAGTTATTTACAGTTACCTTTTTCCTTTA530.11039366798583627No Hit
CATCAGGGAGGTTGCATGCTCCTTATAAGAAACTAACTAATGCCTGATGATC520.10831076858987712No Hit
CTTTAAGGGAAAAAGGGCTGCCTTCCTTCAAATTTTATTTTTGTTTTCTTAA510.10622786919391794No Hit
CGTTTAGGGCAGCTGAGAAGGCTACAAGTGATGAGAAAGATTCTGTTTCAAA500.10414496979795876No Hit
GTTCCAGGCATTTATTACCATAACCATATTTAAATCAATGTGAATCTTACAT500.10414496979795876No Hit
AAATGAGGGAGTTGCTAAAAAGCAAAAAATCTTCCAAATACATCAATATCTA490.10206207040199958No Hit
GCAGTGGGTGATGGACAGAGAGAGACAGAGATAGAAACGGAGACAGAGAGAG490.10206207040199958No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)