FastQCFastQC Report
Sat 31 Dec 2022
SRR5849803.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849803.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59968
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGCCGTCT11861.9777214514407684RNA PCR Primer, Index 36 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGC9081.5141408751334047No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCTTATAC1760.2934898612593383No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCT1090.18176360725720386No Hit
TGGTTCGGGACTCTTTTGGTGGCTTTATCAGATAATTATATCATACCATTGA980.1634204909284952No Hit
CTTGGCGGGAGGGATTACAACCAGCATCTTAATTGATAACAATCCAGTCTGG850.14174226254002134No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACA820.13673959445037354No Hit
AAAATAGGGAGGATTATATAAAAAAAGCACAGGTGCTTAAAAACAACAACAA690.11506136606189968No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGT660.11005869797225186No Hit
CTTTAAGGGGGACAATGATGGAGACGGCGAATGTGACTTCCAGGAATTCATG660.11005869797225186No Hit
CGACAGGGACATGATGTTCGAAGTGATGGAACCGACCTGGCACATCCTGGAG650.10839114194236928No Hit
AAGTGTGGGACCCAAATCTTGGTGCTTCCATATGTTGGCAAGGAACTGAATA650.10839114194236928No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAAACTCGTATGC640.10672358591248667No Hit
ACTATTGGGACCATTTACAGTTGCTCCAGAGAACATGAAATACTTAGGTATA630.10505602988260405No Hit
ACCTAGGGACTTGGTCAGATGTTTATTCTACATTGTTCCCCAGGAACAGAGT600.10005336179295624No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)