FastQCFastQC Report
Sat 31 Dec 2022
SRR5849802.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849802.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59390
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGCCGTCT12052.028961104563058RNA PCR Primer, Index 36 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGC9111.5339282707526518No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCTTATAC1740.2929786159286075No Hit
TGGTTCGGGACTCTTTTGGTGGCTTTATCAGATAATTATATCATACCATTGA1140.19195150698770835No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCT1040.17511365549755853No Hit
CTTGGCGGGAGGGATTACAACCAGCATCTTAATTGATAACAATCCAGTCTGG980.1650109446034686No Hit
CGACAGGGACATGATGTTCGAAGTGATGGAACCGACCTGGCACATCCTGGAG900.15154066341134872No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAAACTCGTATGC850.14312173766627379No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGT760.12796767132513892No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACA750.12628388617612393No Hit
ACTATTGGGACCATTTACAGTTGCTCCAGAGAACATGAAATACTTAGGTATA660.11112981983498904No Hit
ACAACGGGTAAAGTGATTAGTTGAACTGGCATCCTGCTTTAGCCTGAGACTT660.11112981983498904No Hit
AGCTGAGGGATTTGGGACTTGAGATTTGCAGTGATACCAACTTATAGCAATG660.11112981983498904No Hit
GCGAACGGGAACGACAGCCTGGACCAGGCCTACGCAGAGCTGAAGGAGGCGC630.1060784643879441No Hit
CTTTAAGGGGGACAATGATGGAGACGGCGAATGTGACTTCCAGGAATTCATG630.1060784643879441No Hit
AAAATAGGGAGGATTATATAAAAAAAGCACAGGTGCTTAAAAACAACAACAA610.10271089408991413No Hit
GCATGGGGGAATCAACAAACATGAATATAAATGCTTTAGTATGAAGAAGCCA600.10102710894089914No Hit
GAACTCGGGATACTTGTTCTCACAAAATTGTTATGGTCAGTTTATATCATTG600.10102710894089914No Hit
GATCTTGGGACCACCAGGACTCAGAGGCCGCCATCAACCGCCAGATCAACCT600.10102710894089914No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)