FastQCFastQC Report
Sat 31 Dec 2022
SRR5849801.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849801.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59186
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGCCGTCT11561.953164599736424RNA PCR Primer, Index 36 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGC9081.5341465887203054No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCTTATAC1460.2466799580981989No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCT1330.22471530429493464No Hit
TGGTTCGGGACTCTTTTGGTGGCTTTATCAGATAATTATATCATACCATTGA1120.1892339404588923No Hit
CTTGGCGGGAGGGATTACAACCAGCATCTTAATTGATAACAATCCAGTCTGG980.16557969790153076No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAAACTCGTATGC910.15375257662285No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACA760.1284087453113912No Hit
AAAATAGGGAGGATTATATAAAAAAAGCACAGGTGCTTAAAAACAACAACAA750.12671915655729396No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGT680.11489203527861318No Hit
CGTCTGAGAGGGGAGTGGTCAGAAAAGAATTTTCTGAGGAATTGATGTTTTT650.10982326901632143No Hit
GAACTCGGGATACTTGTTCTCACAAAATTGTTATGGTCAGTTTATATCATTG620.10475450275402966No Hit
GTTCCTGGGATTAATTCTCAGATGTGGATATCACTTGGGTCCATATAATGGG620.10475450275402966No Hit
TCAAGAGGGGGACGCGCGCCCTGCGGCCCGGCCTCTAGTCATCGCCCTCGCA600.10137532524583516No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)