FastQCFastQC Report
Sat 31 Dec 2022
SRR5849800.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849800.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110172
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGCCGTCT11301.0256689539992012RNA PCR Primer, Index 36 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGC8230.7470137603020731No Hit
ATGTGGGGGGGCTTCTCAGCAGTAGCATTTATTGAGCACCTGTTAGGATCTA1800.16338089532730637No Hit
CATGTAGGGAAATAAGGCAAACACTGAGCTGCTTCCGTACCATGCTCCCATT1660.15067349235740476No Hit
AACTTGGGGTGGATTGAAGCTGAACAAATAGAAATTGCATTTGGCAATTATT1440.13070471626184513No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCTTATAC1370.12435101477689431No Hit
GATACTGGAAGCTACCAGACTTGTGAGTCAGACTGCCTGGGTTACAATATTT1320.11981265657335803No Hit
TGCGTTGGGGAAAAAATAAAAACAGAATGAGATATAAATTCAAAATATACAA1290.11708964165123624No Hit
CACAACGGGATGGCTGAATAATACTGAGTCCTTCAACTCTGTTCGTCTTTTT1260.11436662672911448No Hit
TGCAGTGGGAGAAACATGGCAAACTGCTCTGTGCTTTCAAACCAAAGTGTTC1250.11345895508840721No Hit
ACAGCTGGGGGGCCTTCCCAACATGGCGCAGTCTATTAACATCACGGAGCTG1200.10892059688487093No Hit
CAGTGAGGGAAGGGGCCAATTCTAAGTATTCTTCACGGCAGCCAGAAGTTAA1170.10619758196274914No Hit
GAAAATGGGTAATTCACAGATGACAGAATTAAGATTATAAAAGATTTTTTTT1170.10619758196274914No Hit
ACGGCCGGTATTGAAATAATTAAAAAATTAAACAGAAGTCTTAGAACTGATA1170.10619758196274914No Hit
TTGACCGGAACACAAAAAAGATTCCATGGCAGGTATTTCTTGGCATGAGACT1170.10619758196274914No Hit
TAGAATGGGTAGTTGGGAATACTTTTAAGTCTCACCTTCCCCTTTAAACTAA1110.10075155211850562No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)