FastQCFastQC Report
Sat 31 Dec 2022
SRR5849799.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849799.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110718
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGCCGTCT11391.0287396809913474RNA PCR Primer, Index 36 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGC8940.8074567820950522No Hit
CATGTAGGGAAATAAGGCAAACACTGAGCTGCTTCCGTACCATGCTCCCATT2020.18244549215123107No Hit
ATGTGGGGGGGCTTCTCAGCAGTAGCATTTATTGAGCACCTGTTAGGATCTA2000.18063910113983273No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCTTATAC1670.1508336494517603No Hit
CAAAAAGGGGCAAACACAGCACTTTCTCAGACTCACTTATAAGAGTGCGTTT1400.1264473707978829No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCT1270.11470582922379378No Hit
GCTGCAATGGGAAATGATCTGTGACAGATAGTTGGAATGTTGTTATTCTGAG1230.11109304720099712No Hit
GCATTTGGGAGAATATCAAAATAATTTGTGGCTGTGGATTTTTTTAACTGCT1230.11109304720099712No Hit
AACTTGGGGTGGATTGAAGCTGAACAAATAGAAATTGCATTTGGCAATTATT1230.11109304720099712No Hit
CACAACGGGATGGCTGAATAATACTGAGTCCTTCAACTCTGTTCGTCTTTTT1220.11018985169529796No Hit
TTGACCGGAACACAAAAAAGATTCCATGGCAGGTATTTCTTGGCATGAGACT1210.10928665618959879No Hit
CAGTGAGGGAAGGGGCCAATTCTAAGTATTCTTCACGGCAGCCAGAAGTTAA1190.10748026517820047No Hit
GAACTGGGGACTGCTTAATGCAGAGACTCACCTGAGGCACACTGATGCACCC1190.10748026517820047No Hit
TGCGTTGGGGAAAAAATAAAAACAGAATGAGATATAAATTCAAAATATACAA1190.10748026517820047No Hit
GAAAATGGGTAATTCACAGATGACAGAATTAAGATTATAAAAGATTTTTTTT1180.10657706967250132No Hit
TAGCTTGGGACTCAATTTGTCTTATGAAAACTCACGGATATGGTCTGTGGGA1160.10477067866110298No Hit
TTTGCAGGGGATTTTATATGCTAAATCATACATCTGTGTTTTGCTGATGAGG1160.10477067866110298No Hit
GATACTGGAAGCTACCAGACTTGTGAGTCAGACTGCCTGGGTTACAATATTT1150.10386748315540381No Hit
ACGGCCGGTATTGAAATAATTAAAAAATTAAACAGAAGTCTTAGAACTGATA1130.10206109214400548No Hit
TTGACTGGGTCTGCTTTTTGGGGGCTAACTTTGGTAGCACACTGTTTTGATT1110.10025470113260718No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)