FastQCFastQC Report
Sat 31 Dec 2022
SRR5849798.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849798.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110274
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGCCGTCT10910.9893537914648965RNA PCR Primer, Index 36 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGC8400.7617389411828718No Hit
ATGTGGGGGGGCTTCTCAGCAGTAGCATTTATTGAGCACCTGTTAGGATCTA1870.16957759762047264No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCTTATAC1560.14146580336253334No Hit
CATGTAGGGAAATAAGGCAAACACTGAGCTGCTTCCGTACCATGCTCCCATT1530.13874530714402308No Hit
CACAACGGGATGGCTGAATAATACTGAGTCCTTCAACTCTGTTCGTCTTTTT1400.1269564901971453No Hit
CAAAAAGGGGCAAACACAGCACTTTCTCAGACTCACTTATAAGAGTGCGTTT1320.11970183361445129No Hit
TGCGTTGGGGAAAAAATAAAAACAGAATGAGATATAAATTCAAAATATACAA1220.11063351288608375No Hit
AACTTGGGGTGGATTGAAGCTGAACAAATAGAAATTGCATTTGGCAATTATT1210.10972668081324699No Hit
ACGGCCGGTATTGAAATAATTAAAAAATTAAACAGAAGTCTTAGAACTGATA1150.1042856883762265No Hit
CTTGTGGGAGGGATGATTAATAATTTTTCTATTTTGAGCCTTCCAGTATATG1150.1042856883762265No Hit
GCTGCAGGGACGTCAACCACCGCAAACACAACATTCCGCACTTCTGGCCCAA1140.10337885630338974No Hit
TTGACCGGAACACAAAAAAGATTCCATGGCAGGTATTTCTTGGCATGAGACT1140.10337885630338974No Hit
TTGACTGGGTCTGCTTTTTGGGGGCTAACTTTGGTAGCACACTGTTTTGATT1140.10337885630338974No Hit
AGCGCTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1120.10156519215771623No Hit
CAGCTTGGGCGATCTAGCAGCGGGAGACTCTGGCTGTGCCGGTTACCATGTG1120.10156519215771623No Hit
GCTGCAATGGGAAATGATCTGTGACAGATAGTTGGAATGTTGTTATTCTGAG1110.1006583600848795No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)