FastQCFastQC Report
Sat 31 Dec 2022
SRR5849797.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849797.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71072
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGCCGTCT9111.2817987393066186RNA PCR Primer, Index 36 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGC6740.9483340837460604No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCTTATAC1560.2194957226474561No Hit
TTTGGCGGGATCTATGAGGATCTTAAATTTACAAGAAAACTACCCCATTAAA1010.14210941017559658No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCT990.13929536244934715No Hit
CTTTGCGGGCAGGCTTCTAATGTAGATGTGAAGATGACGTGGCTAAAAGAAA920.1294461954074741No Hit
TTGTTAGGGACCCGAGCGTGTTGCGGCAGCGGCTGGGCTTGCAAGGCGCGAT890.12522512381809994No Hit
AGTGATGGGATCTTAGTGGGGGAATGTTAGTATAAGTAACATGAAAACATTC880.12381809995497524No Hit
TATTGAGGGATATTTAAGGAAAACATTTAGGTGATTTCTGCAATAACCACTC860.1210040522287258No Hit
GCCCCGGGATAACATAAGAAAAAGAAGAGTACTTAAAATGTGAATGTAAAAC860.1210040522287258No Hit
TAAGCCGGGAATCAACATCAGAAAATTCATACTGGTGTGAAACCCTATGAAT830.11678298063935164No Hit
AATATAGGGGAGGCCTGTTGCACGGCCTCGCCGGGCCTGGCCAGACCCTGCA820.11537595677622693No Hit
GTCGCTGGGGTTTACATTTTGCTTTAGCCTGATGTTTAGTCCTAGCATTCTG810.1139689329131022No Hit
GACTTCGGGGGACGGTGGAACTGAAAAGGACAACTGTATGTATAGACAACTT790.11115488518685278No Hit
AGGCAAGGCAGCGGTGCTTAATCAATATTTCCTGTGCTCACCAGAGGCAAAA770.10834083746060334No Hit
ATTCTGGGGAGAGCGGACCGATCTGGTGCCCTCCGTGGCCCGAGGCCTGCGG760.10693381359747861No Hit
GAGCTAGGGATTTTAGGCTGGACAGTTTTTTGTTGTGGGGTCTGTCCCGTGT750.1055267897343539No Hit
CTTGCAGGGATGTGATAAAATAATAGACGTTAAGGAACTGCAATATTTTCAT740.10411976587122918No Hit
CATATAGGGATTCACGAGAGCTTCACCCTTAAGTCTCAATCACCTCCCAAAG730.10271274200810447No Hit
ATTTATGGGAGATAACACCTTACCACACAGTCTCAACTTAAACACTTCAAAT730.10271274200810447No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)