FastQCFastQC Report
Sat 31 Dec 2022
SRR5849796.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849796.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70526
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGCCGTCT9461.341349289623685RNA PCR Primer, Index 36 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGC6810.9656013385134561No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCTTATAC1610.22828460425942204No Hit
TCCTCAGGGAAAACAGTAATTTCCAAACTTCTGTTCACCATGAAAAGGCAAT950.13470209568102542No Hit
AATATAGGGGAGGCCTGTTGCACGGCCTCGCCGGGCCTGGCCAGACCCTGCA900.12761251169781357No Hit
CAAACAGGGAGCCAGCATCTGAAAAATGCAGAGCAATTCAATTAATCATTGC840.11910501091795933No Hit
CTTGCAGGGATGTGATAAAATAATAGACGTTAAGGAACTGCAATATTTTCAT840.11910501091795933No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCT840.11910501091795933No Hit
TATACAGGGGGTGCACACTTGTAGACCCAGGTACTCTGGAGGCTGAGGTGGA820.1162691773246746No Hit
GGCCATGGGCGACCTGAGGTTTCAGAGCGCAGCCATCGGTGCGCTGCAGGAG820.1162691773246746No Hit
CATATAGGGATTCACGAGAGCTTCACCCTTAAGTCTCAATCACCTCCCAAAG810.11485126052803221No Hit
TGAAATGGGATCTTTTAACTTAGAGATAGCATTTTTTGCCCTCTTTATTTTG780.1105975101381051No Hit
TACTTAGGGACCCCCGGCAGCCGAAACTCTGCCTACACGGAGCTGGGAGACT780.1105975101381051No Hit
GTAGCCGGGATATTAGCTCATTTTACACATAATATACTGAGATTTAGAGTGA780.1105975101381051No Hit
TTTGGCGGGATCTATGAGGATCTTAAATTTACAAGAAAACTACCCCATTAAA780.1105975101381051No Hit
ACACTGCGGGCTCTGCATTCGTTATTGTAAGGTTTTAGATTGGACAATTATC770.10917959334146272No Hit
GACTTCGGGGGACGGTGGAACTGAAAAGGACAACTGTATGTATAGACAACTT760.10776167654482036No Hit
GTCGCTGGGGTTTACATTTTGCTTTAGCCTGATGTTTAGTCCTAGCATTCTG760.10776167654482036No Hit
GTACACGGGACAAATTAATGATATAGCCTATCATTTATAAACAGTGATTGAA750.10634375974817797No Hit
AGTGATGGGATCTTAGTGGGGGAATGTTAGTATAAGTAACATGAAAACATTC750.10634375974817797No Hit
ACTCAGGGGAGCCAGCCCCAGACTCCAGTGCACCAGAGCACGCACAGGAGCC740.10492584295153559No Hit
GGCGCAGGGATGAGTGAGAACATGCAGTGTTTGGTTTTCTGTTCCTCTGTTA740.10492584295153559No Hit
TTGTTAGGGACCCGAGCGTGTTGCGGCAGCGGCTGGGCTTGCAAGGCGCGAT730.10350792615489324No Hit
CTCTTAGGGACATCTGGCAGCAGACTTCTCAGTGGAAAGCTTCCAGGCCAGG730.10350792615489324No Hit
AGGCAAGGCAGCGGTGCTTAATCAATATTTCCTGTGCTCACCAGAGGCAAAA720.10209000935825087No Hit
GCAATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA720.10209000935825087No Hit
ACAACAGGGGTGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC720.10209000935825087No Hit
TAGTCGGGAAGTCATTTGAAAAAAATACAGTATGTAAAATTTGTTCATTCGT710.10067209256160849No Hit
CTGCCATTGGGACTGAAATATAATGAGACAGTTTTACCCATTAAATATAATA710.10067209256160849No Hit
CACAACGGGATGGACCACGGTGTGGTCCTTCCTCACAGTTTTGCTCTTAAGG710.10067209256160849No Hit
ATCGCAGGGATGCCAAGGCCCACGAGGGCTATGTATGTCAAAAAGGATGTTC710.10067209256160849No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)