FastQCFastQC Report
Sat 31 Dec 2022
SRR5849795.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849795.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71284
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGCCGTCT9371.314460467987206RNA PCR Primer, Index 36 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGC6880.965153470624544No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCTTATAC1310.183771954435778No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCT890.12485270186858202No Hit
GGCCATGGGCGACCTGAGGTTTCAGAGCGCAGCCATCGGTGCGCTGCAGGAG850.11924134448123001No Hit
AGTGATGGGATCTTAGTGGGGGAATGTTAGTATAAGTAACATGAAAACATTC850.11924134448123001No Hit
TTACTTGGGACAAACTGGCAATGCACAATAAACTAAAATAACCTACTAATAA820.11503282644071602No Hit
TATTGGGACTTTCTTTTCTTTGTCTCTTGCTGCTTTCTTCTGACTTTTGAGC810.113629987093878No Hit
CTTGCAGGGATGTGATAAAATAATAGACGTTAAGGAACTGCAATATTTTCAT790.110824308400202No Hit
AAAATGGGATGGTGGTTTGTGAAGATTCGAGTTTTAGAATTTGGGTTTCTCT770.10801862970652601No Hit
TCCGCTGGGAGATAAAGGGAAACATCTTGAGCTAAATTAGACGAGAATAAAT770.10801862970652601No Hit
TAAGCCGGGAATCAACATCAGAAAATTCATACTGGTGTGAAACCCTATGAAT770.10801862970652601No Hit
TGACCATCGGGGAGATAATTCTAGTACAAAGTATAATAAAACTAGATGTATA760.10661579035968802No Hit
TTTGGCGGGATCTATGAGGATCTTAAATTTACAAGAAAACTACCCCATTAAA760.10661579035968802No Hit
GTGCTTGGGACAGCAGGCAAAGATGATGAAGAAGAAGATTATTATTTTAAGG750.10521295101285001No Hit
TATACAGGGGGTGCACACTTGTAGACCCAGGTACTCTGGAGGCTGAGGTGGA740.10381011166601202No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAAACTCGTATGC740.10381011166601202No Hit
CTATGCCGGGTGTCAAGCAAGGCAAACCAAGAAAAATCAAAGTCTAAACATC730.102407272319174No Hit
GTTGGTGGGACTCCAGTATAACTTTTTGTTGATAGTAGTTCAGTGGGATAGA730.102407272319174No Hit
TTGTCAGGGACAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTATTAATG720.10100443297233601No Hit
GAGCTAGGGATTTTAGGCTGGACAGTTTTTTGTTGTGGGGTCTGTCCCGTGT720.10100443297233601No Hit
CTGCCATTGGGACTGAAATATAATGAGACAGTTTTACCCATTAAATATAATA720.10100443297233601No Hit
CTCTTAGGGACATCTGGCAGCAGACTTCTCAGTGGAAAGCTTCCAGGCCAGG720.10100443297233601No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)