FastQCFastQC Report
Sat 31 Dec 2022
SRR5849794.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849794.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67878
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGCCGTCT7031.0356816641621733RNA PCR Primer, Index 36 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGC5330.7852323285895283No Hit
GCCTCTGGGAGATGGTGGTAATTTTTATTCCCTTCTCTGCAATTTCAGCATA1420.20919885677244468No Hit
TCTTTGGGGGGATTTTGTCTTTTTAAAAGATTATCTATGAATGCCAGTTTTG1220.17973422905801587No Hit
GATATGGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGT1200.17678776628657297No Hit
TGCTAGGGGAAGATGGAGAAATTTAGCAGAGAACTAGAAGCTAAAAAAAGAA1180.17384130351513008No Hit
ATTGATGGGATCGGTCCTGTTAGGAGGGGAGAAAAAGTTCTTCCAAAGGCTG1160.17089484074368722No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCTTATAC1090.1605822210436371No Hit
GAAAAAGGGCTTTATTTCCATCTATGGTGTTGCAAATGACTGGATTTTATTT1060.15616252688647278No Hit
AACCGAGGGGGAAAATAAAGAAATATCCCTTGGCCAGGTGACAAAAATATCT1040.15321606411502992No Hit
AGTTGTGGGATATTTTTATCTTTGTATAATTTGGTTTGGCTTTTCCTTGCCA1030.15174283272930847No Hit
AACACCGGGAACCATCTTCTCCTTACACCTAGCAGGTGTCTCCTCTATCTTA1030.15174283272930847No Hit
AGCTATGGGATCACTTGCTGAGATGGGAAAGAGTTTAGGGAAGGAGGAAATT990.1458499071864227No Hit
TGCAACTGGGCTGTAAACTGGAGTTAGCTCTAAATGCTTGATTATTTGGGTC990.1458499071864227No Hit
ATCTGTGGGGAATTCATATTTTTTCTTGGACAATATGCTAATATTTATTGAT970.14290344441497982No Hit
GTCTACGGGAACTTTATAAAACTAAGAACAAGCTTATAATTCATTTTTAAGC960.14143021302925837No Hit
AGAAGTGGGATGCCACCACAATACATTTTAATCTCACGTTTCAAAATACAAA960.14143021302925837No Hit
TCTCGTGGGAGAGACATAATTATCAGAATTTAAAAATCTGAGTAACCAGATT950.13995698164353695No Hit
GCACCAGGGACTGCCCGCAGCCCTGGTTCCCACCCACGCCTCTCCCTCCACA950.13995698164353695No Hit
CAGTATGGGAGAGCAGGAGGCCGACGTTTTCTCCGCATTGGAAGAGCCCGCC940.1384837502578155No Hit
GTGAGGGGTAAATTTTACTTTAGTAAAGTAAAACAAATTTTCTCTTATTTTT940.1384837502578155No Hit
ATTGCGGGGAGGAGATCTGCAGAAGAGGGGCCTGACTGTTAGAAGGAAGACT940.1384837502578155No Hit
GTACAGGGGATTCTACTCCCCAAAGGAAGTCTCACCATAGGCCTGTGTAGAC930.13701051887209406No Hit
GCAATCGGGAGAATGGGAGGTTAGAGAAAGATTAATGCGACGGACTTAAAAT930.13701051887209406No Hit
GCTCGAGGGATCTGCTCCTGCGTTTCACGCATCTGCATACCTTTGGTTACTC930.13701051887209406No Hit
CTTACAGGGAATTTGAAGGCGATCTTTTAAAAAGAGATTAAACCGAAGGTGA910.13406405610065117No Hit
CAAAATGGGAGGGGAGATAGTCACCACCATTCCTACCATTGGTTTTAATGTG910.13406405610065117No Hit
TACTACGGGGGGTTCAGGAGATGGCTCTGAGGAGCAGTTCAGGTTGGGAGGG910.13406405610065117No Hit
GACGTTGGGGTAACAGATCAATAGTCCAAAAAAGTGTTTAGTAAATTAGGTT910.13406405610065117No Hit
TACAAAGGGTGAGATGGTCAGCCGGGTCATGAAAGCACTGGTAAACCGCAAG900.13259082471492972No Hit
CTAATGCGGGATGCTAGTTTAACCCTACTATTAAAGCCTTACCTTTTGGTGT890.13111759332920828No Hit
AGCGATGGGGTTTCACTCCTTTCCATTTCTTTCTAAAATTACAGTAAAATAT890.13111759332920828No Hit
AACACTGGGCTTTGCTCACTCTGAGCAACAATGCCAATTATTCATGCAATCA890.13111759332920828No Hit
ATAGTGGGGATGCAGAAAAAATTGAATCTTTATTGCTGATGGGAATATAAAA890.13111759332920828No Hit
AACGTTGGGAGGTGGTAAGTTTTCTTCATACAGTTCTTTTGTGTGACAAACT880.12964436194348683No Hit
AAACTAGGGGACTGATTAAAAATGCTAAACTCCTATATAGTAAAAAATAACA880.12964436194348683No Hit
GCATTGGGAACTTATTGAAATTAACAGAAAATTTCTATTAGTAAAATAGAAT870.12817113055776538No Hit
TGAGTAGGGGGATGGACAGCTTCGGCGGTGGGAAGGGAAGGGACATGCTGCA870.12817113055776538No Hit
TTGACGGGGGTGTAAGGAGGCATTTTGTGTGTCTTTGCAATGTATACAATTG870.12817113055776538No Hit
TGATGAGGGTATCATCCATCTTTTAGAATCCCAGGGAGCTAATTTCTGGTCC870.12817113055776538No Hit
TGGTGGCGGACTCCATTTCTCTCAAAAGCCCTCAAGTCACAAAGTAAATGGT860.12669789917204397No Hit
TATATGGGGGACACGGGAAGGCCAGCTACAGGTGGACTCCTGGAATTTGAGG860.12669789917204397No Hit
ACTCCAGGGATTTTTACTAAGTTTAGTCTTAGAGATTTGACCATCCACCAAA860.12669789917204397No Hit
AAAACTGGGAGTGCTAAGTATGCCACTAAGTTTCAGATATATGGAATATTTT860.12669789917204397No Hit
CCGTCAGGGGTGATACATTTACCAAAAAACCTTATTAAGGCAATAACTAAAA850.12522466778632252No Hit
TAAAATGGGACAGGCTGTTATTTTTCTCAATACGAGGCGCAAGGTGGACTGG850.12522466778632252No Hit
CTCAAGGGGACGCTCTTCCGATCTGACCAAGGGGTTTCTTTGTTTACTTCAT840.12375143640060107No Hit
AACAATTGGGGATATGGCAGGGCAGATATTCAAAGGGAGTTACAAAAGGACA840.12375143640060107No Hit
AACACCGGGATGCCGATCCAACTTAATTGCCAGGCAACTCTTCTGCACTTTA840.12375143640060107No Hit
GAGGGGGAGATCCGCTGCTGCTGAGGAGAGGAGCGTCAACAGCAGCACCATG840.12375143640060107No Hit
TTAGACCGGGGAGGGTGGGCTGCTGGAAGGCGCGACTGCAACTTGACTTTGC830.12227820501487963No Hit
TTGTTTGGGAACAGCTAATGGAACCTTACTGAAAACCCATTTGCATTAATGC830.12227820501487963No Hit
GCCCCAGGGATTTCCAAAAGTTGTAAACTTTGATGGCTGATTTTTCGTAAGT830.12227820501487963No Hit
CAGAAAGGGAGAGATGATTGAATTTTATTATTTTTCCCCAAATCTTTTCCAG830.12227820501487963No Hit
AAGTGAGGGAGAGGCAGGGTAGCTTGGATAGGAGACTCAGTGAGATTTTGTG830.12227820501487963No Hit
TGAACGGGGACAGGACCTGTGGGAGGGACTATCAGAGATGCAAAAATTACTT830.12227820501487963No Hit
GGTAAAGGGAAGGTAGATTGTTTGAAGCAGAAGTCAGTTCTCCTGGAGCTCA820.12080497362915821No Hit
ATACTTGGGAGTGTTTAGAGGGAAATTTATAGCACTAAATGCCCATAGGAGA820.12080497362915821No Hit
AAGAAAGGGAATTCATGTGATAGCTAATATATTTGGTTTTAAATGCATCTTA820.12080497362915821No Hit
CGCACAGGCATCTTCCTAAAATAAGTGAGTGGGGCCATGTGATCTCAAAAAT820.12080497362915821No Hit
ATGCTAGGGATAGCAACTAACAGGGAGAGCGTGGGTTGAGAGGGAAATCAAT820.12080497362915821No Hit
ATTTTCGGGGTTTTGCATATGACTGCTGCTACAAATTAAAACCCAAATAGAC810.11933174224343676No Hit
GTAAACGGGGGAGCAGAAAGGATCACAGATGTGGCTTTGGATTTCTGGAGGA810.11933174224343676No Hit
ACTATAGGGATGGGCGGGATGGCCTATTTCATAGAAATGAAAATTTATGTGT810.11933174224343676No Hit
TGCGAGGGATCCCCAAGCCAACATACAATGTGAAATGAAAGCCAGTGCGTGG810.11933174224343676No Hit
AGCTAAGGGAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATA810.11933174224343676No Hit
CCAGCCTGGGGGACAGAGCGGGACTGTCTCAAAAACAAACAAACAAACAAAC800.11785851085771532No Hit
CATCCAGGGATCGTGAAAATGGCCATACTGCCCAAGGTAATTTATAGCTTCA800.11785851085771532No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCT800.11785851085771532No Hit
TCTATTGGGATCTTAGCGCGGGGCCTCAAACGATGAATTGTAGAATGGAGCA800.11785851085771532No Hit
TTTATGGGGTAGTACAGAATATCTTATTCAGATTACTTTTGAATAACCAGAA790.11638527947199387No Hit
TGGCAGGGATTTCTGCAGGTAGAATAAATATCCTATTTAAAGCTTTCAAATC790.11638527947199387No Hit
GCGTGAGGGAGGGTCAGAGGCAGGGACAGATGTGAGTATGCTATGCTGCTGG780.11491204808627242No Hit
GCAAATGGGTGGATTCTAAAATTTTACAATGAATCATCCACAGTGCCACTAT780.11491204808627242No Hit
GGCATTGGGTCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAAT780.11491204808627242No Hit
TGAAATGGGGGTGCGCGCCGGCCGCGGCTTCAGCCTGGAGGAGCTCAGGGTG780.11491204808627242No Hit
GGCATTGGGATTTTCTCATCATTTAATGTGATGTAGCTGTGGACTTTTGGAA780.11491204808627242No Hit
TGCTGAGGGAAGTGTTAGAAAAATCTATATCTTAAAACACAAAACTGTAACA770.11343881670055099No Hit
AAGTATGGGAATCCTAAGGAACATCCAGTAAAACAGGAATTGGAAAGAATCA770.11343881670055099No Hit
AGGGCTGGGATGTACAATTATTAAATGTCAACTAAAAATAAAAGGAAAAAAA770.11343881670055099No Hit
TAGGTCCGGGACTATGAAATTATCTAAAAAATGCTCATAGGGTTCCTAATGT770.11343881670055099No Hit
GTTTGAGGGATACTGTGTGTACCATGACTGCAGACTAGAGTTATGCCAACCT770.11343881670055099No Hit
ACAGATGGGATTGTTACTTCTTTTATATATAATTACTTTTGCACATAAGCAT770.11343881670055099No Hit
CATTTAGGGGCCTTTCTCTGGTATCCTCTTGGCATGTGTAACATACATGTAG770.11343881670055099No Hit
TGTCATGGGCGATCTAAAGACGGGGTGTATTATTGAATTTTAAGCAATATTT770.11343881670055099No Hit
GAACCTGGGACTGCCTGGATAGCCTGGACCAGGACAGCTTCGACACCATCCA770.11343881670055099No Hit
GCTTAGCGGGATAGAGAGACTTCATCTCTACCCAAAATCAACAAAAAGCCAA770.11343881670055099No Hit
TAACTCGGGAAGTTGCAAAAGTTGGGGCTGCAATTAATGCTAACATAAGAGC760.11196558531482954No Hit
CAGCTTGGGATACATGCTCTTTACAGAGCGTTTTTAGCTTTATAAATGACTT760.11196558531482954No Hit
GTGCAAGGGGTATTTATCCCTGAACATGTTTTGTACCTTTTTTTTTTTTTTT760.11196558531482954No Hit
GTAACTGGGATGGAAGGAGGAATTCAAAGGAATCATGTCTTCAGTGTGTTTC760.11196558531482954No Hit
ACGCTTGGGACTCCAATTACTTTTCCATTCTGCACAATGATTTCTTCAATGG760.11196558531482954No Hit
TACTTAGGGGAGTACAGAGTAGTTATTTGAGAGCATGAATTCTGGAGCTGGA760.11196558531482954No Hit
ACTCACGGGACTCTTAGGGGCCATGGTCAAGTTCAATAAAATAAAAGATTCC750.1104923539291081No Hit
TCGTCAGGGATCATCCTGATACCAAAACCTGGCAGAGACTCAACAAAAAACG750.1104923539291081No Hit
GTAGGAGGGATTGAAGGTGTAACTTGATAAAACTGTACCTGCGTTGCAGTTT750.1104923539291081No Hit
CCGTAAGGGGGGAAATGACAGATGAGAGAGAATACAAATGTGGACGTAAATA750.1104923539291081No Hit
GACTGACGGGATGTGGATTATGAACAAATGAAAGTTTGCTGTGTGATTGCAG750.1104923539291081No Hit
TTGCAAGGGCAGTCACAGGAAAAGAGTGGAAAAGTAGTAGTATTTGAAATGG740.10901912254338665No Hit
CTTATATGGGCTAAGACCTCTGATCATTAAAACCTATAAAGTGTTGAGCTGT740.10901912254338665No Hit
GCATCTGGGGACCCTTGACTTCTGATCTTTGTCTACTTCATTTAGCTGAGCA740.10901912254338665No Hit
AATGAAGGGCTTCCGATCTTGAGATGGGGGAAGAGGCCGGGCATTGGTGGTC740.10901912254338665No Hit
CGTGCCGGGATGATGGGGCCTGCTGTTCCCTGAGCATTCCTTTCATTCCTCA740.10901912254338665No Hit
AAGACTGGGACTTGGACAGAGGGATTTGTGTGGATTGCAAATTAAGTCTTCA740.10901912254338665No Hit
TCGAACGGGGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGGAG740.10901912254338665No Hit
AGGTCCGGGACAATAATACTTAACTCAGAATATTATTGGATTCTGCTTTTCT730.10754589115766523No Hit
AGACTTGGGATAAAAGAAAACAAGAAGAAGAATTAACAAAAAGAATGCTGGA730.10754589115766523No Hit
TGATGATGGGACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCA730.10754589115766523No Hit
TCGCACGGGTCTCTGAATCGAGGCACTACCTCAATTCTCGGCTTAGTTATTT730.10754589115766523No Hit
ATTTCTGGGAATGCTACTTAATTGCAGTTTTTTCCCCAACAAGTTAATAGTT730.10754589115766523No Hit
GTAACTGGGTCTGAGCAAATTAATGGGTACCTTTTTTTTCTTATTTAAAGCA730.10754589115766523No Hit
GTGCTGGGGATGTACAGTTGAGCCTTGAACAAAGTGTGTTTGAACTGCCTGA730.10754589115766523No Hit
GCAGCCGGGAAGGAATATATTGACATCTGGGAAGAGTTAGATCCACCCTTGT730.10754589115766523No Hit
GGCGCAGGGCTTGACATCTACGTTTAAGAAGGGAACGTGGAAGAGAAGGTGA720.10607265977194379No Hit
GGTTGTGGGAATTTATACCATGACCAAGGAACTGATTTGTAATCAGAGCTCA720.10607265977194379No Hit
CACCGAGGGTCTGTGCGAGGGAGGACCCCAGAAGAACGGGCCGCCGCCGCCA720.10607265977194379No Hit
AAAATGGGGATGGCTTAACTTTCTCTTATGTTTTCTTAGAATATGTGTAAAT720.10607265977194379No Hit
CGCTGGGGGATGCCAACAAGGACACTGACCACTCCAACTCTATCTGACAGGA710.10459942838622234No Hit
TACATAGGGGAGAACACCGAGCACTGAACTTTGCAAAGACCTTCGTCTTTGA710.10459942838622234No Hit
GGTTGTGGGGAGAATCCACAATACAGTAGTGTGGATAACTTTCAAGAAGCCA710.10459942838622234No Hit
TGCTGTGGGGCTTTCTTTCCTTTGACTTCTTCCTGAGGCTTCTTTTCTTTAT710.10459942838622234No Hit
AGGCTTGGGTGCTCACAACATGCTCAGAGTTCATCAGTTAGCTGTGACCCAG710.10459942838622234No Hit
GAATTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG710.10459942838622234No Hit
CTCGATGGGGTATTGTGACTGGGTTGGATAAAGACATTGATTCTAACGGGTC710.10459942838622234No Hit
ATTGGTGGGAGTGCCAGCCTCTGTGGCAGTCCCAATGCCAATCCCGTGGAGC710.10459942838622234No Hit
GCTACTGAAGGGCAAAATGCTCTGTGATATATACATGATATGTTAATCATTA710.10459942838622234No Hit
CTCAAAACGGGATTAACCAAAATAACAATGATAATAGTTCATTTTAAAAGTT700.1031261970005009No Hit
AATTATGGGGTTTCTCTTCGGTTTGAATTCTCTCATGGTTACCAAAACTCTG700.1031261970005009No Hit
ATAGCAGGAGAAATGTGAACCCCTGCCACTGAGGAAGATGAAGGTAGACCCT700.1031261970005009No Hit
CTGTGTGGGGATCTTATATATACATATGCATGGATATATGATTTAATTGTAC700.1031261970005009No Hit
CAACATGGGAAGTCAGTAATATTGTGTTCCAATTCTTGTAGCTTTATGAATA700.1031261970005009No Hit
ATGACCGGGAGCCAGTTCCGTAAATAACTTTAAAATTCTAAAAGTGTTGGTA700.1031261970005009No Hit
GGTTTTGGGAGACTCTTGAGTTTTGTAACTTTTCTCCCTTTGCATCAATATC700.1031261970005009No Hit
TGGATAGGGAGGATACAAGTACATGCTTTGTTGTCTGTGCATGTACCAGATA700.1031261970005009No Hit
TATAAGGGGACTAGTCCAATGTTACAAAATAATTGGAAAAAAGATGACAGAC700.1031261970005009No Hit
TAACCAGGGATCTACTAACGGGATATAACAAGAATAAACATTTAATAAGAGG690.10165296561477945No Hit
ATAAGGGCTATCACCAGTGCCTATGGAATGAAAAGATTTTCTATTCCACTAA690.10165296561477945No Hit
TTTCAGGGGGTGATCTCATTGTTCAATTCCCACCTATGAGTGAGAATATGCG690.10165296561477945No Hit
CAATATGGGAGACCTAAGCCCTCCCCCACCCCTGGAAAGGTCCCTCCCCAAC690.10165296561477945No Hit
TGTACAGGGATCTCCTTTCTAGGCTTTCTACTTTCAGGCAAAAGCTGTTGAT690.10165296561477945No Hit
ACAACGGGGTGGCTTGAACTTGCCAGATGCAAATACCACAGACTCCAAGAAA690.10165296561477945No Hit
TTATCTGGGATGCGTATTTCCTCTACACACATCACCCCCCTTCTATAATCTT690.10165296561477945No Hit
TTGGACGGGGGGCCCTGGGGGAGGGGTCTCACGTCCCCTGGGGAAGCGCCGG680.10017973422905803No Hit
TGCTCGGGGATTCACCAAGTGGATATCAGTTTTCATTCTTAGGTTCAAAAAT680.10017973422905803No Hit
CTCATAGGGATCTCTGTCTATAAACTTTAGGAAAATATGTAAAATCTTGTTC680.10017973422905803No Hit
AACGAAGGGGGCCTGCGTAGCCGGCGGGCTGGCAGTGAGACTGACTGCGTCG680.10017973422905803No Hit
GCTATAGGGGGTGGCTGTTTTTGTAAATGTACAAACTTAGCAAAAAAATCAC680.10017973422905803No Hit
AGCTTTGGGATCTATAGAAATGATGCCAAATTTTTTACCCTAATTAATAAAA680.10017973422905803No Hit
AGTTGCGGGTACCAAGACGTCTGCTTTTGTGACAGTCAGAAAACACACCTGG680.10017973422905803No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)